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1

Long-term in vitro storage of shoot explants at temperatures above 0C

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Lisek A., Orlikowska O. T., Orlikowska T.
Biotechnologia
|
2001
|
issue 3
134-144
EN
Long-term storage of shoots or nodal buds in vitro, in conditions which retard their growth, may be an alternative or supplementary method in the protection of plant resources and in the production of planting material for elite mother stocks. In this paper we describe the most important factors which influence the survival and further behaviour of stored explants.
2

Analysis of plum (Prunus domestica L.) DNA polymorphism using RAPD - PCR technique

100%
Lisek A., Korbin M., Zurawicz E., Rozpara E.
Biotechnologia
|
2004
|
issue 2
76-72
EN
Precise identification of plum cultivars is desired in breeding programme of the species as well as in orchard practise. The aim of the presented studies was the determination of 19 plum cultivars Prunus domestica L. diversity. RAPD-PCR technique with 77 primers (Operon Technologies) from kits OPB, OPG, OPT and OPU was used for the analysis. Fifty-five polymorphic fragments DNA (600-2700 bp) were obtained in reactions with 33 primers. The highest number of polymorphic fragments (3-5) was observed in reactions with primers OPB 07, OPB 18, OPG 09, OPG 10 and OPT 14. The reactions with OPB 07, OPB 18 and OPG 09 allowed to diversify 15 cultivars, except 'Wegierka Zwykla' and clones 'Promis', 'Tolar', 'Nectavit'.
3

Effect of 6-benzylaminopurine, cold-hardening and time of transfer of cultures to the storage room on long-term storage of raspberry ?Malling Seedling? shoots in vitro

100%
Lisek A., Orlikowska O. T., Orlikowska T.
Biotechnologia
|
2001
|
issue 3
237-242
EN
Shoots of raspberry ?Mallig Seedling? originating from multiplication cultures were stored in vitro at 4C in the dark on a medium with 0,4 mg/l or without 6-benzylaminopurine (BAP), for 18 and 24 months. The cultures were transferred to the storage room immediately after the subculture or after one-week incubation in the growth chamber. A part of the shoots was cold-hardened for one week. Almost all the shoots survived 24 months of storage. The shoots stored on the medium containing BAP formed shoots during storage. Shoot multiplication was poor during the first passage, especially in the result of 24 months storage. Time of transfer and hardening did not influence multiplication capacity instantly.
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