The influence of two animal and plant proteases was investigated in vitro on 3rd stage Anisakis simplex B larvae.The larvae were placed in 3 series of 5 sterile test-tubes containing 0.1% solu-tions of tyrosine, pepsin and papain. Physiological saline solution was used as control. It was found that Anisakis simplex B larvae are the most resistant to pepsin treatment.
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