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Qualitative and quantitative analysis of gallic acid in Alchemilla vulgaris, Allium ursinum, Acorus calamus and Solidago virga-aurea by chip-electrospray ionization mass spectrometry and high performance liquid chromatography

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Condrat D., Mosoarca C., Zamfir A., Crişan F., Szabo M., Lupea A.

Open Chemistry

2010

vol. 8

issue 3

530-535

This study presents the results obtained from qualitative and quantitative analysis of gallic acid from hydro-alcoholic extracts (methanol, ethanol) of plants from Plantae regnum. Plant qualitative analysis was performed using a novel mass spectrometric (MS) method based on fully automated chip-nanoelectrospray ionization (nanoESI) high capacity ion trap (HCT) while quantitative analysis was carried out by high performance liquid chromatography (HPLC). These methods were applied to Alchemilla vulgaris - common lady’s-mantle (aerial part), Allium ursinum - bear’s garlic (leaves), Acorus calamus - common sweet flag (roots), Solidago virga-aurea - goldenrod (aerial part). Obtained results indicated that methanol extracts (96%, 80%) have a gallic acid content ranging between 0.0011–0.0576 mg mL−1 extract while the ethanol extracts (96%, 60%) exhibit a gallic acid concentration that varies between 0.0010–0.0182 mg mL−1 extract. [...]

Fully automated chip-based nanoelectrospray combined with electron transfer dissociation for high throughput top-down proteomics

100%

Flangea C., Schiopu C., Capitan F., Mosoarca C., Manea M., Sisu E., Zamfir A.

Open Chemistry

2013

vol. 11

issue 1

25-34

The conventional protocol for protein identification by electrospray ionization mass spectrometry (MS) is based on enzymatic digestion which renders peptides to be analyzed by liquid chromatography-MS and collision-induced dissociation (CID) multistage MS, in the so-called bottom-up approach. Though this method has brought a significant progress to the field, many limitations, among which, the low throughput and impossibility to characterize in detail posttranslational modifications in terms of site(s) and structure, were reported. Therefore, the research is presently focused on the development of procedures for efficient top-down fragmentation of intact protein ions. In this context, we developed here an approach combining fully automated chip-based-nanoelectrospray ionisation (nanoESI), performed on a NanoMate robot, with electron transfer dissociation (ETD) for peptide and top-down protein sequencing and identification. This advanced analytical platform, integrating robotics, microfluidics technology, ETD and alternate ETD/CID, was tested and found ideally suitable for structural investigation of peptides and modified/functionalized peptides as well as for top-down analysis of medium size proteins by tandem MS experiments of significantly increased throughput and sensitivity. The obtained results indicate that NanoMate-ETD and ETD/CID may represent a viable alternative to the current MS strategies, with potential to develop into a method of routine use for high throughput top-down proteomics.

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2 Open Chemistry

1 2013

1 2010

2 Mosoarca C.

2 Zamfir A.

1 Capitan F.

1 Condrat D.

1 Crişan F.

1 Flangea C.

1 Lupea A.

1 Manea M.

1 Schiopu C.

1 Sisu E.

1 Szabo M.

Search results

Qualitative and quantitative analysis of gallic acid in Alchemilla vulgaris, Allium ursinum, Acorus calamus and Solidago virga-aurea by chip-electrospray ionization mass spectrometry and high performance liquid chromatography

Fully automated chip-based nanoelectrospray combined with electron transfer dissociation for high throughput top-down proteomics