The influence of the rye genome on triticale pre-harvest sprouting (PHS) resistance was studied by using Presto substitution lines, where rye chromosomes were substituted by the D genome of wheat. The PHS resistance was evaluated on the third, sixth and ninth day of a mist chamber test as a percentage of germinated kernels. All the substitution lines, except 6D(6R), showed a higher PHS resistance than cv. Presto, which means that the rye component of triticale influences negatively the triticale PHS resistance. The 2D(2R) line was the most resistant (finally 16% of sprouted grains). In all the lines, except 5D(5R), the sprouting dynamics was nearly linear during the experiment. The lowest increase in number of sprouted kernels (up to 7%) was observed in lines 3D(3R), 2D(2R) and 6D(6R) within the first three days of the mist-chamber test, but at the end of the experiment line 6D(6R) showed the highest PHS susceptibility (56% of sprouted grains). The fastest grain germination in spikes was observed for the 5D(5R) line. Thus a simple and cheap modernization of the mist-chamber test, by additional evaluation of the lag phase and the initial germination in spikes during the first three days, is suggested for selection of genotypes with higher potential of PHS avoidance.
Allogeneic hematopoietic cell transplantation (alloHCT) is the treatment of choice for various pediatric malignancies and nonmalignant diseases. The most prominent complication of allotransplantation is graft vs host disease (GvHD). The treatment of GvHD influence negatively function of immune system and increase risk of bacterial, fungal and viral infections. Clinical symptoms of viral infection may mimic GvHD and lead to inappropriate treatment. Human ctomegalovirus (CMV, Herpesviridae) has been recognized as most important viral pathogen after alloHCT. Increasing number of procedures, especially from alternative donors, requiring more intensive immunosuppression, led to identification more viral pathogens causing transplant related mortality and morbidity. Among them are adenoviruses (ADV, Adenoviridae), BK and JC viruses (Papovaviridae) and human herpes virus 6 (HHV-6, Herpesviridae). Frequency of complications caused by those pathogens is higher in children then in adults.
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