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EN
Various actions have been taken in object to improve human diet in iron with high bioavailibility. One of the new possibility of food fortification is introduction phytoferritin into. In order to do this various strategies are used as increasing of native ferritin supply in human diets as far as application genetic engineering for receiving cultivated plants with high expression of this plant. The most important feature of ferritin introducing into food is its high bioavailability.
EN
The article presents the principles of liquid-liquid extraction in aqueous two-phase systems which offer a great potential for the recovery and purification of various biomolecules, cells and organelles from fermentation slurries. These systems consisting of a solution of two polymers (e.g. polyethylene glycol/dextran), polymer and salt (e.g. polyethylene glycol/phosphate) or a thermoseparating polymer (oxyethylene-oxypropylene copolymer) offer mild separation conditions due to the high concentration of water, which makes them suitable for biotechnology applications.
EN
This paper is focused on actual and potential applications of aqueous two-phase systems in the separation of biotechnology products. Applications of bioconversions, purification of enzymes and other biomolecules in these systems, proposed schemes of separation, and advantages and disadvantages are presented. The experimental and industrial results indicate high efficiency of the separations of biological materials in aqueous two-phase systems, which may result in the development of new technologies.
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