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ISOLATION OF CHITIN FROM APLYSINA AEROPHOBA USING A MICROWAVE APPROACH

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Klinger C., Żółtowska-Aksamitowska S., Jesionowski T.

Progress on Chemistry and Application of Chitin and its Derivatives

2019

vol. 24

61 - 74

Chitin of poriferan origin represents a unique renewable source of three-dimensional (3D) microtubular centimetre-sized scaffolds, which have recently been recognized as having applications in biomedicine, tissue engineering, and extreme biomimetics. The standard method of chitin isolation from sponges requires concentrated solutions of acids and bases and remains a time-consuming process (lasting up to seven days). Here, for the first time, we propose a new microwave-based express method for the isolation of chitinous scaffolds from the marine demosponge Aplysina aerophoba cultivated under marine farming conditions. Our method requires only 41% of the time of the classical process and does not lead to the deacetylation of chitin to chitosan. Alterations in microstructure and chemical composition due to the microwave treatment were investigated using various analytical approaches, including Calcofluor White staining, chitinase digestion, scattering electron microscopy, and Raman and ATR-FTIR spectroscopy. It was demonstrated that microwave irradiation has no impact on the chemical composition of the isolated chitin.

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1 Progress on Chemistry and Application of Chitin and its Derivatives

1 2019

1 Jesionowski T.

1 Klinger C.

1 Żółtowska-Aksamitowska S.

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ISOLATION OF CHITIN FROM APLYSINA AEROPHOBA USING A MICROWAVE APPROACH