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Differentiation by random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) of Candida albicans isolated from upper respiratory tract in patients with non-small cell lung cancer

100%
Biernasiuk A., Korona-Głowniak I., Grzegorczyk A., Malm A.
Acta Biochimica Polonica
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2014
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vol. 61
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issue 4
727-729
EN
Cancer patients are predisposed to fungal infections caused by Candida albicans, especially to oral or respiratory tract candidiasis. The aim of this study was to estimate genetic diversity by RAPD-PCR (random amplified polymorphic DNA-polymerase chain reaction) of C. albicans isolated from upper respiratory tract of 100 patients with non-small cell lung cancer. Among 52 strains, 34 genotypes were defined. 10 clusters comprising 28 (53.85%) isolates with similarity coefficient ≥ 80% were formed. The remaining 24 (46.15%) isolates represented individual genotypes. The RAPD-PCR technique revealed genomic variability within C. albicans isolated from upper respiratory tract of the cancer patients.
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Antimicrobial activity of petroleum ether and methanolic extracts from fruits of Seseli devenyense Simonk. and the herb of Peucedanum luxurians Tamam.

88%
Widelski J., Grzegorczyk A., Malm A., Chinou I., Głowniak K.
Current Issues in Pharmacy and Medical Sciences
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2015
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vol. 28
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issue 4
257-259
EN
Plants of the Apiaceae family usually contain coumarins. These are used worldwide in traditional medicine, as well as in modern therapeutics. The aim of our study was to determine the antimicrobial activity of four extracts (methanolic and petroleum ether extracts) obtained from two Apiaceae species: Seseli devenyense (fruits) and Peucedanum luxurians (herb). The activity of the investigated extracts was tested against 7 strains of Gram+ bacteria, and 7 strains of Gram-, as well as three of yeast. The results of this show that the best activity of such extracts (specifically, by way of petroleum ether) was seen as being against Staphylococcus aureus strains.
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