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Initial Phases of DNA Rehydration by NMR and Sorption Isotherm

100%

Harańczyk H., Czak J., Nowak P., Nizioł J.

Acta Physica Polonica A

2010

vol. 117

issue 2

397-402

T*he initial stages of rehydration of salmon sperm deoxyribonucleic acid (DNA) lyophilizates were observed using hydration kinetics, sorption isotherm, and high power proton relaxometry (at 30 MHz). T*he hydration kinetics reveals (i) a very tightly bound water not removed by incubation over silica gel (A_0^{h} = 0.057 ± 0.010), (ii) a tightly bound water [saturating at A_1^{h} = 0.149 ± 0.007, hydration time t_1^{h} = (0.27 ± 0.08) h], a tightly bound water (iii) [saturating at A_2^{h} = 0.694 ± 0.039, with the hydration time t_2^{h} = (9.8 ± 3.2) h], and (iv) a loosely bound water fraction for the samples hydrated at p/p_{0} ≥ 76% [with the hydration time t_3^{h} = (44 ± 14) h, and the contribution progressively increasing with the air humidity]. For the hydration at p/p_{0} = 100%, after t_{0} = (244 ± 22) h of incubation the swelling process begins. T*he amount of additional water uptake at swelling depended on the macrostructure of the sample. Sorption isotherm is sigmoidal in form and is fitted well by the Dent model with the mass of water saturating primary binding sites Δ M/m_{0} = 0.114. Proton free induction decay is a superposition of the immobilized proton signal (Gaussian, with T*_{2S} ≈ 20 μs) and two liquid signal components coming from tightly bound (T*_{2L_1} ≈ 100 μs, with the mass saturating at Δ m/m_{0} = 0.111 ± 0.044) and loosely bound water fraction (with the amplitude proportional to the mass of water added).

Rehydration of CTMA Modified DNA Powders Observed by NMR

64%

Harańczyk H., Kobierski J., Zalitacz D., Nowak P., Romanowicz A., Marzec M., Nizioł J., Hebda E., Pielichowski J.

Acta Physica Polonica A

2012

vol. 121

issue 2

485-490

The rehydration of salmon sperm deoxyribonucleic acid (DNA) and cetyltrimethylammonium chloride (C_{19}H_{42}ClN) complexes was observed using hydration kinetics, sorption isotherm, and high power proton relaxometry (at 30 MHz). The hydration kinetics shows (i) a very tightly bound water not removed by incubation over silica gel (A_0^{h} = 0.061 ± 0.004), (ii) a tightly bound water saturating at A_1^{h} = 0.039 ± 0.011, with the hydration time t_1^{h} = (1.04 ± 0.21) h, a loosely bound water fraction (iii) with the hydration time t_2^{h} = (19.1 ± 3.2) h and the contribution progressively increasing with the air humidity. For the hydration at p/p_0 = 100%, after t_0 = (152.6 ± 2.5) h of incubation the swelling process begins. The swelling time was t_3^{h} = (12.5 ± 5.4) h, and the swelling amplitude A_3^{h} = 0.140 ± 0.016. The sorption isotherm is sigmoidal in form and is fitted by the Dent model with the mass of water saturating primary binding sites Δ M/m_0 = 0.102 ± 0.021. Proton free induction decay is a superposition of the immobilized proton signal (Gaussian, with T_{2S}* ≈ 30 μs) and two liquid signal components coming from tightly bound (T_{2 L_1}* ≈ 100 μs) and loosely bound water fraction with the amplitude proportional to the mass of water added (T_{2 L_2}* ≈ 1000 μs).

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2 Acta Physica Polonica A

1 2012

1 2010

2 Harańczyk H.

2 Nizioł J.

2 Nowak P.

1 Czak J.

1 Hebda E.

1 Kobierski J.

1 Marzec M.

1 Pielichowski J.

1 Romanowicz A.

1 Zalitacz D.

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Initial Phases of DNA Rehydration by NMR and Sorption Isotherm

Rehydration of CTMA Modified DNA Powders Observed by NMR