Full-text resources of PSJD and other databases are now available in the new Library of Science.
Visit https://bibliotekanauki.pl

Refine search results

Journals help

  1. 1 Biotechnologia

Years help

  1. 1 1999

Authors help

  1. 1 Grundland C.

Preferences help
Polish version English version Language
enabled [disable] Abstract
Number of results

Results found: 1

Number of results on page
first rewind previous Page / 1 next fast forward last

Search results

help Sort By:

help Limit search:
first rewind previous Page / 1 next fast forward last
1

Mutagenesis of mouse embryonic stem (ES) cells

100%
Grundland C.
Biotechnologia
|
1999
|
issue 1
94-105
EN
Embryonic stem (ES) cells derived from preimplantation mouse embryo provide a powerful tool for genome manipulation in mammals. The two principal genetic approaches are used to modify genomes of embryonic stem cells, which may be introduced into blastocyst to produce chimeras, and these animals transmit the genetic alteration into the next generation. One approach, targeted mutagenesis, is designed to disrupt the function of specific murine genes that are known by their homology to genes of other organisms. The other approach, gene trapping by randomly insertional mutagenesis, is designed to identify novel, developmentally regulated genes in mouse embryos. In vivo screens allow for the identification and studying of genes that are expressed either within specific tissue or in spatiotemporal patterns. As an alternative to in vivo gene study, gene expression within specific cell types may be monitored in different ES cell cultures.
first rewind previous Page / 1 next fast forward last
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.