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Use of Mixed Feeds for Inducing Gonad Maturation of G4 Transgenic Mutiara Catfish Broodstock Indoor Hatchery

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Buwono I. D., Apriliani I. M., Herman R. G., Napsiah I. S.
World News of Natural Sciences
|
2023
|
vol. 51
1-9
EN
This research aims to determine the optimal dose of boiled mackerel tuna mixture on HI-PRO VITE 781 feed on gonadal maturity index and feed conversion ratio of transgenic mutiara catfish G4 in indoor hatchery maintenance at optimal temperature. This research was conducted at the Hatchery of the Faculty of Fisheries and Marine Sciences, Universitas Padjadjaran. The method used was an experimental method with a completely randomized design (CRD) method with four treatments, namely the difference in the dose of boiled mackerel tuna administration in comercial feed and three replications. The treatment in this study was the administration of boiled mackerel tuna doses of 3.5%, 2.5%, 1.5% in mixed feeds to G4 transgenic mutiara catfish and tested on non-transgenic mutiara catfish with a percentage of 3.5% female weight biomass and then added an 30 g HI-PRO-VITE 781 with two times feeding frequency. The research parameters included gonad maturity index and feed conversion ratio. The results showed that feeding a mixture of boiled mackerel tuna and HI-PRO-VITE 781 had a significant effect on the gonadal maturity index and feed conversion ratio. Feeding boiled mackerel tuna at 1.5% was the best treatment with a gonadal maturity index of 47.16% for females and 4.98% for males and a feed conversion value of 1.19 for 56 days of rearing.
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Animal cloning by nuclear transfer: state-of-the-art and future perspectives

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Dinnyes A., Szmolenszky A.
Acta Biochimica Polonica
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2005
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vol. 52
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issue 3
585-588
EN
Model organisms are essential to study the genetic basis of human diseases. Transgenic mammalian models, especially genetic knock-out mice have catalysed the progress in this area. To continue the advancement, further sophisticated and refined models are crucially needed to study the genetic basis and manifestations of numerous human diseases. Coinciding with the start of the new era of post-genomic research, new tools for establishment of transgenesis, such as nuclear transfer and gene targeting in somatic cells, have become available, offering a unique opportunity for the generation of transgenic animal models. The new technology provides important tools for comparative functional genomics to promote the interpretation and increase the practical value of the data generated in numerous mouse models. This paper discusses the state-of-the-art of the nuclear replacement technology and presents future perspectives.
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