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Microbial amylases: A review

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Microbial amylases are enzymes produced by microorganisms to hydrolyze starch. There are three types of microbial amylases: alpha-amylase, beta-amylase and glucoamylase. Each of these amylases has a unique way of acting on starch to yield simple glucose monomers. Microorganisms, plants, and animals are sources of amylases, but much attention is given to microorganisms since the amylases produced by them have greater thermal stability and give rise to different sugar profiles, thus meeting industrial demands. Two major groups of microorganisms play pivotal role in amylase production: bacteria and fungi. Starch is the substrate used in amylase production. Between the two fermentation processes used in amylase production (i.e. submerged fermentation and solid state fermentation), the latter is more advantageous as it saves cost, generates little effluent, and has high volumetric productivity. Microbial amylases are greatly applied in pharmaceutical, food, chemical, paper and distilling industries.
EN
Pleurotus pulmonarius (Fr.) Quel. is a mushroom species that occurs widely in nature on all continents except Antarctica. It is most common in North America. Its fruiting bodies are characterised by a mild taste and a slight anise aroma. These mushrooms are valued as a source of nutrients and substances with a healing effect. The anticancer, anti-inflammatory and antioxidant properties of P. pulmonarius have been scientifically proven, as well as its strong antihyperglycemic activity. P. pulmonarius is easy to grow because it has a very aggressive mycelium towards cellulosecontaining materials. In Poland, it can be grown on substrates based on cereal straw and various types of organic waste, including agricultural, horticultural, textile and forestry. In intensive crops, the substrates are also enriched with protein and carbohydrates. On an industrial scale, P. pulmonarius is grown primarily in Asia and North America on locally available organic materials.
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The major aim of the newly adopted Mercury Convention is to reduce global mercury (Hg) emissions to the environment. In high temperature industrial processes, including coal combustion, Hg compounds present as impurities in solid materials are decomposed and evaporated leading to the emission of Hg to the atmosphere. The behaviour of different Hg compounds and their mixtures during heating have been the subject of numerous studies, and is the topic of the present work. Controlled heating can be used to fractionate Hg compounds in solid substrates, offering the possibility of identification and quantification of Hg compounds. In the attempt to develop a method for temperature fractionation of Hg, experiments were conducted with pure Hg compounds, and the compounds mixed with different substrates (SiO2 and CaSO4 • 2H2O), for calibration purposes. Detection was performed by two methods, namely Cold Vapour Atomic Absorption Spectrometry (CV AAS) with Zeeman background correction, and Nier-type Mass Spectrometry with a Knudsen cell (MS). Further investigation is in process.
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