Search results

1

Molecular identification of Babesia spp isolated from Polish cattle with asymptomatic protozoa infections

100%

Staniec M., Adaszek Ł., Buczek K., Winiarczyk S.

Polish Journal of Veterinary Sciences

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2018

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vol. 21

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issue 2

2

Cloning of the lymphoid enhancer binding factor-1 (Lef-1) cDNA from rat kidney: Homology to the mouse sequence

100%

Kobielak K., Kobielak A., Trzeciak W. H.

Acta Biochimica Polonica

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1999

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vol. 46

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issue 4

885-888

EN

We have cloned and sequenced rat cDNA that encodes the Lef-1 protein. The cDNA, containing 1194 nt exhibits 94% similarity to the mouse Lef-1 cDNA. The deduced amino-acids sequence of rat Lef-1 protein, consisting of 397 amino acids, exhibited 98% homology with the known sequence of mouse Lef-1 protein.

3

Differentiation of Bacillus anthracis and other Bacillus cereus group bacterial strains using multilocus sequence typing method

75%

Graniak G., Olender A., Naylor K.

Acta Universitatis Lodziensis. Folia Biologica et Oecologica

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2020

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vol. 16

12-21

EN

The study describes the preparation of the phylogenetic differentiation of Bacillus cereus strains. The Bacillus cereus group of bacteria is very important for human and animal health. The multilocus sequence typing scheme has been used to present this group of bacteria’s phylogenetic relationship and structure. The MLST system was established using 60 isolates of B. anthracis, B. cereus sensu stricto, B. thuringiensis, and transitional environment strains of Bacillus spp. As a negative control, five strains of B. subtilis and B. megaterium were used. Primers for amplification and sequencing were designed to target highly conserved internal fragment of seven housekeeping genes: glpF, gmk, ilvD, pta, pur, pycA, and tpi. A total of 22 different sequence types (STs) were distinguished. Analysis of the sequence data showed that all of the Bacillus cereus strains are very closely related. The MLST scheme exhibited a high level of resolution that can be used as an excellent tool for studying the phylogenetic relationship, epidemiology, and population structure of the Bacillus cereus group strains. The MLST method additionally allows us to define the phylogenetic relationship between very closely related strains based on a combination of the sequences of all seven alleles fragments and each of them separately. Thus, this genetic investigation tool is very useful in epidemiological investigation of potential military/ bioterrorist use of B. anthracis.

4

Prevalence and molecular characterization of Salmonella species associated with piglet diarrhea in North East India

75%

Kylla H., Dutta T. K., Roychoudhury P., Subudhi P. K., Lalsiamthara J.

Polish Journal of Veterinary Sciences

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2019

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issue 4

5

Buffer management in solving a real sequencing problem in the automotive industry – Paint Shop 4.0 concept

75%

Bysko S., Krystek J., Bysko S., Lenort R., Bysko S., Krystek J., Bysko S., Lenort R.

Archives of Control Sciences

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2019

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vol. 29

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issue 3

6

Preliminary result of a genetic polymorphism of β-lactoglobulin gene and the phylogenetic study of ten balkan and central european indigenous sheep breeds

75%

Kusza S., Sziszkosz N., Nagy K., Masala A., Kukovics S., András J.

Acta Biochimica Polonica

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2015

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vol. 62

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issue 1

109-112

EN

Genetic polymorphism at the β-lactoglobulin (β-LG) loci in indigenous sheep breeds (Tsigai, Racka, Pramenka) was determined. Altogether 904 sheep were genotyped for the presence of the A, B and C alleles of β-lactoglobulin by PCR-RFLP. The AB genotype was the most common and the β-lactoglobulin A was the most frequent in the Cokanski Tsigai (54%), while the B allele was the most common in the Rusty and the Zomborski Tsigai (59%, 60%). The C allele was found only in one individual from Serbian Cokanski flock. These results differ from those that refer to other native sheep breeds. In the Cokanski Tsigai, deviation from the Hardy-Weinberg equilibrium was detected. Genetic relationship based on β-lactoglobulin polymorphism was the closest between the Rusty and the Cokanski Tsigai among the studied populations and between sheep and goat among the other ruminants. Part of the promoter region (254 bp) of β-LG in studied sheep breeds were sequenced in order to identify polymorphisms, analyze haplotypes, and phylogenetic relationship among them. Sequencing analysis and alignment of the obtained sequences showed one haplotype. Analysis of more samples and longer parts of the promoter region of β-LG are needed to reconstruct a phylogenetic tree.

7

The present state of herbicide resistance of weed populations in the Czech Republic

63%

Salava J., Chodová D.

Journal of Plant Protection Research

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2007

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vol. 47

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issue 4

8

Compound heterozygous LDLR variant in severely affected familial hypercholesterolemia patient

63%

Al-Allaf F., Alashwal A., Abduljaleel Z., Taher M., Bouazzaoui A., Abalkhail H., Al-Allaf A., Athar M.

Acta Biochimica Polonica

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2017

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vol. 64

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issue 1

75-79

EN

Familial hypercholesterolemia (FH) is most commonly caused by mutations in the LDL receptor (LDLR), which is responsible for hepatic clearance of LDL from the blood circulation. We described a severely affected FH proband and their first-degree blood relatives; the proband was resistant to statin therapy and was managed on an LDL apheresis program. In order to find the causative genetic variant in this family, direct exon sequencing of the LDLR, APOB and PCSK9 genes was performed. We identified a compound heterozygous mutation in the proband with missense p.(W577C) and frameshift p.(G676Afs33) variants at exons 12 and 14 of the LDLR gene respectively. DNA sequencing of LDLR gene from the parents demonstrated that the missense variant was inherited from the mother and frameshift variant was inherited from the father. The frameshift variant resulted in a stop signal 33 codons downstream of the deletion, which most likely led to a truncated protein that lacks important functional domains, including the trans-membrane domain and the cytoplasmic tail domain. The missense variant is also predicted to be likely pathogenic and affect EGF-precursor homology domain of the LDLR protein. The segregation pattern of the variants was consistent with the lipid profile, suggesting a more severe FH phenotype when the variants are in the compound heterozygous state. The finding of a compound heterozygous mutation causing severe FH phenotype is important for the genotype-phenotype correlation and also enlarges the spectrum of FH-causative LDLR variants in the Arab population, including the Saudi population.

Refine search results

Molecular identification of Babesia spp isolated from Polish cattle with asymptomatic protozoa infections

Cloning of the lymphoid enhancer binding factor-1 (Lef-1) cDNA from rat kidney: Homology to the mouse sequence

Differentiation of Bacillus anthracis and other Bacillus cereus group bacterial strains using multilocus sequence typing method

Prevalence and molecular characterization of Salmonella species associated with piglet diarrhea in North East India

Buffer management in solving a real sequencing problem in the automotive industry – Paint Shop 4.0 concept

Preliminary result of a genetic polymorphism of β-lactoglobulin gene and the phylogenetic study of ten balkan and central european indigenous sheep breeds

The present state of herbicide resistance of weed populations in the Czech Republic

Compound heterozygous LDLR variant in severely affected familial hypercholesterolemia patient