Search results

1

An improved method for RNA isolation from plants using commercial extraction kits

100%

Kalinowska E., Chodorska M., Paduch-Cichal E., Mroczkowska K.

Acta Biochimica Polonica

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2012

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vol. 59

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issue 3

391-393

EN

Isolation of RNA from plants rich in secondary metabolites using commercial kits often results in contaminated preparations which are not suitable for downstream applications. Although many specific protocols appropriate for plants with a high content of phenolics, anthocyanins and polysaccharides have been developed, these are often expensive, time consuming and not applicable to different types of tissues. This study presents a simple and efficient modification of RNA extraction from different types of tissues using two commercial reagent kits. By simple improvement, we routinely obtained high-quality RNA of the following plants: the blackcurrant bush, black chokeberry bush, pear tree, apricot tree, apple tree, hardy kiwi, tangerine tree, highbush blueberry and cranberry plant.

2

The evaluation of the in-vitro antimicrobial activity of Alangium salvifolium (Linn)

70%

Khan M. N.

World News of Natural Sciences

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2018

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vol. 20

176-181

EN

Finding healing power in plants is an ancient idea, and people of all continents and civilization have been using plants in one form or the other for poultices or as decoctions. Alangium salvifolium (Linn), family Alangeaceae, is a tree that grows in the wild throughout India. The plant has been used in the Indian traditional system of medicine for skin diseases (e.g. leucoderma), articular diseases, and anti-inflammation, anti-poisonous, anti-pyretic, and anti-emetic requirements. However, no scientific evidence is available regarding its antimicrobial activity. An investigation of Alangium salvifolium as an antimicrobial activity agent is the objective of our present study. The ATCC culture used in this study was collected from department of Microbiology, G.M.C., Bhopal (M.P.). Shade dried crude powder (200 grams) of seed of Alangium salvifolium was separately extracted with methanol in a soxhelt apparatus. The Antimicrobial activities were then studied by applying the Disc–Diffusion method. Our results indicate that the observed antimicrobial activity of the fraction appears to be due to unknown secondary metabolites in it. H.P.L.C. (high performance liquid chromatography) and chemical studies may, thus be useful in analyzing the presence of unknown secondary metabolites in the fractions.

3

Bioinformatic insight into Portulaca oleracea L. (Purslane) of Bulgarian and Greek origin

61%

Balabanova V., Hristov I., Zheleva-Dimitrova D., Sugareva P., Lozanov V., Gevrenova R., Balabanova V., Hristov I., Zheleva-Dimitrova D., Sugareva P., Lozanov V., Gevrenova R.

Acta Biologica Cracoviensia s. Botanica

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2020

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vol. 62

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issue 1

4

The effectivity of metabolite secondary extract of bacteria associated with sea grass (Cymodocea rotundata) for Vibriosis treatment in tiger shrimp (Penaeus monodon)

61%

Lili W., Maulana G., Astuty S., Rosidah R.

World News of Natural Sciences

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2019

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vol. 26

96-115

EN

The natural occurrence of the shrimp (Penaeus monodon Fabricius, 1798) and seagrass (Cymodocea rotundata Asch. & Schweinf.) is found in the area of Indian Ocean, Indonesia, for central Pacific. Vibriosis can cause death in larvae, juvenile and adult shrimp almost close to 100%. Antibacterial substances from secondary metabolites are produced by plants and animals to reduce the use of synthetic antibiotics. The purpose of this research was to determine the ability of secondary metabolites contained in the bacterial extract of the association of sea grass Cymodocea rotundata (isolate code BA.1) to treat vibriosis in tiger shrimp (Penaeus monodon). This research was conducted at the Laboratory of Integrated Biotechnology and Laboratory of Aquaculture, Fisheries and Marine Sciences Faculty, Universitas Padjadajaran, and at the Brackish and Southern Ocean Aquaculture Development Center Pangandaran, from June to September 2018. The research method used was experimental, with completely randomized design (CRD) consisting of five treatments with three replications including; control treatment (concentration of 0 mg·L–1), 150 mg.L–1, 300 mg·L–1, 450 mg·L–1 and 600 mg·L–1. Observations made include clinical symptoms (morphology, behavior) and survival rate. Clinical symptoms of infected tiger shrimp during the in vivo test were red spots on the abdomen and necrosis of some leg and tail segments. The behavior of tiger shrimp mostly shows less responsive movements to fish-feed on the first day, and showing responsive and active behavior on the fifth day. In vivo test results for 14 days showed that the highest survival rate of 51.67% was in case of the addition of antibacterial extracts of BA.1 with a concentration of 300 mg·L–1.

5

Isolation, identification and screening of Actinobacteria in volcanic soil of Deception Island (the Antarctic) for antimicrobial metabolites

61%

Yoke−Kqueen C., Learn−Han L., Catherine C. C. Y., Michael W. V. L. C.

Polish Polar Research

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2015

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issue 1

6

Recent developments in Neem (Azadirachta indica – A. Juss) derived antimicrobial constituents for control of human and plant diseases – a review

51%

Dohroo A., Karnwal A., Ghai M.

Annales Academiae Medicae Silesiensis

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2016

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vol. 70

220-223

EN

Neem has immense biocontrol potential against a large number of pathogens causing various diseases in human beings and plants. The plant has maximum useful value in its leaves, bark,, flowers and fruits than any other tree species. These values include antiallergic, antidermatic, antifeedent, antifungal and anti-inflammatory properties. Due to the vast activities of neem, it has become extremely valuable in making our ecosystem a green treasure. Neem leaves have been demonstrated to exhibit immuno-modulatory, anti-inflammatory, antiulcer, antimalarial properties. Neem tree plant parts contain a promising pest control substance which has found effective use against economically important agricultural pests. Neem plant products are easy to process by village level industries on the micro scale level and use by resource poor farmers in crop protection resulting thereby in income generation. This plant also has antioxidant properties and free radical scavenging potential in its different extracted solvents. The present review highlights the properties of neem against the pathogens of living organisms for environmental protection.

Refine search results

An improved method for RNA isolation from plants using commercial extraction kits

The evaluation of the in-vitro antimicrobial activity of Alangium salvifolium (Linn)

Bioinformatic insight into Portulaca oleracea L. (Purslane) of Bulgarian and Greek origin

The effectivity of metabolite secondary extract of bacteria associated with sea grass (Cymodocea rotundata) for Vibriosis treatment in tiger shrimp (Penaeus monodon)

Isolation, identification and screening of Actinobacteria in volcanic soil of Deception Island (the Antarctic) for antimicrobial metabolites

Recent developments in Neem (Azadirachta indica – A. Juss) derived antimicrobial constituents for control of human and plant diseases – a review