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Sampling, metadata and DNA extraction - important steps in metagenomic studies

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Felczykowska A., Krajewska A., Zielińska S., Łoś J.
Acta Biochimica Polonica
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2015
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vol. 62
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issue 1
151-160
EN
Metagenomic studies have become increasingly popular. They allow for the estimation of biodiversity in complex populations. This diversity presents an enormous but largely unexpected genetic and biological pool and can be exploited for the recovery of novel genes, entire metabolic pathways and their products. Generally metagenomic study is a genomic analysis of organisms by direct extraction and cloning of DNA from their natural environment. The most common problems of modern metagenomics are as follows: majority of the microorganisms present in the environment cannot be cultivated by standard techniques, DNA extraction methods are not very effective, isolated DNA is contaminated with various compounds, a choice for a screening method is not obvious.
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Residues and transfer of triazine herbicides in ground waters of intensively exploited arable land in Wielkopolska province of Poland

84%
Drożdżyński D.
Journal of Plant Protection Research
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2006
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vol. 46
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issue 2
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