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1

QUERCETIN ATTENUATES OXIDATIVE STRESS, INFLAMMATION AND CARDIAC DYSFUNCTION IN ACRYLAMIDE- INDUCED CARDIOTOXICITY

100%

Kushwah A. S., KALIA T. S.

Acta Poloniae Pharmaceutica - Drug Research

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2020

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vol. 77

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issue 2

343-352

EN

Acrylamide (ACR) is a foodborne toxic agent, formed in food when processed at high temperature. This study aimed at evaluating the biochemical changes induced by ACR and the effect of Quercetin as a treatment against ACR induced cardiotoxicity in rats. Wistar rats of either sex (n=6) were divided into four groups as follows: normal control, an Acrylamide control group, Quercetin groups (25 and 50 mg/kg). Diagnostics characteristics were assessed daily, at the end of the study (4 weeks) evaluate hemodynamic parameters, the blood sample was collect for estimation of biochemical and rats were decapitated excised hearts, cleaned and weighed. Heart homogenate was used to determine antioxidants and oxidative levels, and histopathological evaluations were carried out to determine changes induced by Acrylamide. As compared control groups, ACR treated rats show altered significantly (P < 0.05) general characteristic and also elevated myocardial damage markers, altered hemodynamic, oxidative stress level, increased expression of inflammatory cytokines and induced histopathological changes. Treatment with Quercetin at 25 mg/kg and 50 mg/kg recouped the above changes significantly (P < 0.05), 50 mg/kg being more prominent. The present study has concluded that Quercetin protects against Acrylamide-induced cardiotoxicity.

2

Antidiabetic cataract effects of GbE, rutin and quercetin are mediated by the inhibition of oxidative stress and polyol pathway

100%

Lu Q., Hao M., Wu W., Zhang N., Isaac A., Yin J., Zhu X., Du L., Yin X.

Acta Biochimica Polonica

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2018

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vol. 65

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issue 1

35-41

EN

One of the earliest critical secondary complications of diabetes is the opacification of the eye lens - a condition strictly associated with diabetic cataract. The study presented here was designed to investigate the effect of Ginkgo biloba extract (GbE), rutin and quercetin on streptozotocin (STZ) induced diabetic cataract (DC) rats. Ten weeks after administration of GbE, rutin and quercetin, the opacity of diabetic rats' lenses was graded under a slit lamp. Then, the levels of malondialdehyde (MDA), reduced glutathione (GSH), advanced glycosylation end products (AGEs), and the activities of aldose reductase (AR) were estimated. The DC-induced rats produced less GSH, higher levels of MDA and AGEs as well as elevated AR activity when compared to the normal group. Administration of GbE, rutin and quercetin remarkably inhibited the AR activity, stimulated the production of glutathione, and decreased the levels of MDA and AGEs in the lenses of DC-induced rats, which eventually delayed the progression of lens opacification in diabetic rats to various degrees. Our results revealed that quercetin had the highest significant (P<0.05) potential to delay the progression of STZ-induced diabetic cataract when compared with rutin and GbE. The mechanism dictating this interesting prowess of quercetin might be attributed to its AR inhibitory strength, anti-lipid peroxidation potential and anti-AGEs activity.

3

The effect of quercetin on oxidative DNA damage and myelosuppression induced by etoposide in bone marrow cells of rats

88%

Papież M.

Acta Biochimica Polonica

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2014

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vol. 61

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issue 1

7-11

EN

There is increasing evidence for the existence of an association between the presence of etoposide phenoxyl radicals and the development of treatment-related acute myeloid leukemia (t-AML), which occurs in a few percent of patients treated with this chemotherapeutic agent. The most common side effect caused by etoposide is myelosuppression, which limits the use of this effective drug. The goal of the study was to investigate the influence of antioxidant querectin on myelosuppression and oxidative DNA damage caused by etoposide. The influence of quercetin and/or etoposide on oxidative DNA damage was investigated in LT-12 cell line and bone marrow cells of rats via comet assay. The effect of quercetin on myelosuppression induced by etoposide was invetsigated by cytological analysis of bone marrow smears stained with May-Grünwald-Giemsa stain. Etoposide caused a significant increase in oxidative DNA damage in bone marrow cells and LT-12 cell line in comparison to the appropriate controls. Quercetin significantly reduced the oxidative DNA damage caused by etoposide both in vitro and in vivo. Quercetin also significantly protected against a decrease in the percentage of myeloid precursors and erythroid nucleated cells caused by etoposide administration in comparison to the group treated with etoposide alone. The results of the study indicate that quercetin could be considered a protectively acting compound in bone marrow cells during etoposide therapy.

4

Protective effects of quercetin on cadmium fluoride induced oxidative stress at different intervals of time in mouse liver

75%

Zargar S., Siddiqi N., Al Daihan S., Wani T.

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issue 2

207-213

EN

Quercetin, a member of the flavonoid family is a major antioxidant acquired in humans by food consumption, while Cadmium fluoride (CdF2) is one of the naturally occurring chemicals having adverse effects. The protective effect of quercetin on time dependent oxidative damage induced in mice liver by CdF2 was studied in the following groups of mice consisting of six mice each: (i) control group; (ii) mice treated with single i.p injection of 2 mg/kg bw CdF2 for 24 h; (iii) mice treated with single i.p injection of 2 mg/kg bw CdF2 for 48 h; (iv) mice treated with single i.p injection of quercetin (100 mg/kg bw); (v) mice treated with i.p injection of 100 mg/kg bw of quercetin followed by i.p injection of CdF2 (2 mg/kg bw) for 24 h; and (vi) mice treated with i.p injection of 100mg/kg bw of quercetin followed by CdF2 (2 mg/kg bw) for 48 h. Administration of quercetin two hours before CdF2 significantly reduced the biochemical alterations in reduced glutathione, ascorbic acid, lipid peroxidation, super oxide dismutase, catalase and total protein (p<0.05). Histopathology also showed the protective effect of quercetin. The livers treated with CdF2 were atrophic, markedly nodular, inflamed and necrotic. However, this effect was reduced to a minimum in the mice pre-treated for two hours with quercetin.

5

QUERCETIN AMELIORATES INSULIN RESISTANCE CONCOMITANT EARLY CARDIOVASCULAR CHANGES IN EXPERIMENTAL RATS

75%

KUSHWAH A. S., GUPTA G. D.

Acta Poloniae Pharmaceutica - Drug Research

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2018

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vol. 75

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issue 4

977-987

EN

Quercetin is a dietary flavonoid found in a wide range of fruits and vegetables; it has diverse biological activities, possesses beneficial effects in ameliorating diabetic complications, apart from the effect of quercetin on fructose feed induced insulin resistance (IR) linked cardiac dysfunction have not been entirely revealed. This study aspires to explore the effect of quercetin on metabolism, oxidative stress, cardiomyocytes damage and cardiac function in IR state. Wistar rats either sex weighing 220-250 g (n ꞊8), were divided into four groups, kept on either control diet and high fructose diet and supplement with a quercetin as a test drug and metformin as a standard, at the dose of 50 and 200 mg/ kg; p.o., respectively. Daily measured body weight, feed, and water intake for 35 days, Oral glucose tolerance test (OGTT) performed in animals on the 32nd day. At end of the study (36th day), measured hemodynamic parameters after that estimation of various biochemical parameters. Finally, the animals were sacrificed for isolation of tissues and measured heart weight, the oxidative stress level of heart and histopathological changes. Treatment of quercetin with fructose-fed ameliorated all the parameters revile by the contrast of IR rats. The outcome of quercetin associated improves insulin sensitivity, normalized lipid profile, abolish hemodynamic changes, oxidative stress and cardiac injury markers within fructose-fed, and lesser histopathological changes were observed contrast with IR rats. These beneficial effects of quercetin mediated by improving insulin sensitivity and metabolism; reduced oxidative stress could potentially be used to ameliorate the myocardial damage.

6

Antioxidant Activity and Mechanism in Flower of Hylocereus Undatus (Haw.) Britt. Et Rose

75%

Li X., Gao Y., Han W., Lin J., Hu Q., Chen D.

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vol. 55

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issue 1

7

VALIDATION OF HEPATOPROTECTIVE USE OF POLYGONUM PERFOLIATUM EXTRACT AGAINST PARACETAMOL INDUCED TOXICITY IN WISTAR RATS

75%

Saleem M., Mushtaq M. F., Akhtar M. F., Saleem A., Zahid S., Sharif A., Akhtar B., Dar E., Ullah M., Badshah M.

Acta Poloniae Pharmaceutica - Drug Research

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2019

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vol. 76

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issue 2

283-289

EN

The liver as a vital body organ is adversely affected by hazardous chemicals and drugs. Paracetamol widely used as analgesic and antipyretic drug produces severe hepatotoxicity at high doses. Present study was designed to investigate the hepatoprotective activity of Polygonum perfoliatum L. used on folklore basis. Aqueous methanolic extract of the plant was prepared. Preliminary phytochemical and HPLC analyses were carried out to identify and quantify chemical constituents respectively. For hepatoprotective activity, Wistar rats were divided into six groups as normal control, standard (silymarin) control, negative control and extract treated groups i.e., 125, 250 and 500 mg/kg/day per oral. Paracetamol was administered orally, following seven days of previously stated therapy. Biochemical parameters of hepatotoxicity such as serum glutamic pyruvate transaminase (SGPT), serum glutamic oxaloacetate transaminase (SGOT), alkaline phosphatase (ALP) and total bilirubin were measured in all groups. Histopathological evaluation of liver was also carried out. Benzoic acid, chlorogenic acid, gallic acid, m-coumaric acid, quercetin and vitamin E were detected in the plant extract through HPLC. The hepatoprotective effect of 500 mg/kg/day therapy was more pronounced than 125 and 250 mg/kg dose. However, the effect of plant extract was less pronounced than standard silymarin therapy. It can be concluded that the plant extract possessed significant hepatoprotective activity that may be attributed to quercetin, benzoic acid, gallic acid and vitamin E present in it.

8

The antioxidant quercetin protects HL-60 cells with high myeloperoxidase activity against pro-oxidative and apoptotic effects of etoposide

75%

Papież M., Krzyściak W.

Acta Biochimica Polonica

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2014

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vol. 61

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issue 4

795-799

EN

The protective action of quercetin against the pro-oxidant and apoptotic effect of etoposide was investigated in HL-60 cells with a high level of myeloperoxidase (MPO) activity and in cells treated with MPO inhibitor, 4-aminobenzoic acid hydrazide (ABAH). Quercetin significantly protected MPO-rich cells against the pro-oxidative (p<0.05) and apoptotic (p<0.05) effects of etoposide. Pre-treatment with ABAH abolished this protective influence of quercetin on apoptosis induced by etoposide but actually enhanced the action effect of quercetin against etoposide-generated reactive oxygen species (ROS) level by this cytostatic drug. Thus quercetin can protect HL-60 cells against the pro-oxidative activity of etoposide regardless of MPO activity.

9

Quercetin influences BSA alpha-helical structures of native, ACR- and NaNO2-modified BSAs

75%

Rorbach-Dolata A., Żurawska-Płaksej E., Piwowar A.

Acta Poloniae Pharmaceutica - Drug Research

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2018

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vol. 75

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issue 6

1339-1346

EN

Quercetin (QUE) is a plant flavonoid with a multifarious spectrum of properties. It is a prominent component of the human diet, considered to be safe and beneficial for human health. Acrylamide (ACR) and sodium nitrate III (NaNO2) are also present in the diet and may demonstrate adverse and toxic effects on the macromolecules and tissues of the human organism. Albumin, the most abundant blood protein, is the most susceptible to the action of various exogenous factors, which may lead to structural damage and functional disturbances. The aim of this study was to estimate ACR- and NaNO2-induced changes in the secondary structure of bovine serum albumin (BSA), using circular dichroism (CD), and to determine the impact of quercetin on these modifications. BSA was incubated with ACR and NaNO2 solutions in the absence and presence of QUE in two different concentrations (3 mM and 500 µM), and changes in albumin alpha-helical structure were determined by CD. BSA secondary structure was vulnerable to alterations upon treatment with acrylamide and NaNO2, as well as quercetin. QUE, depending on concentration and incubation time, caused a decrease of around 13-19% in the alpha-helix content of BSA molecules, but also prevented the changes in the protein alpha-helical structure initiated by ACR and NaNO2. The most spectacular inhibition was revealed for QUE in lower concentrations after 24h of incubation with NaNO2. Although QUE reveals protective effect towards albumin modifications, it is difficult to unambiguously define whether this effect is advantageous, because quercetin itself causes alterations in BSA structure.

10

Potencjalne wykorzystanie substancji pochodzenia roślinnego w leczeniu choroby Alzheimera

63%

Pypno D., Zdrojewicz Z.

Pediatria i Medycyna Rodzinna

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2015

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vol. 11

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issue 3

289-294

EN

Alzheimer’s disease is the most common neurodegenerative condition in elderly people which is characterised by progressing dementia. Patients present weaker episodic memory, attention and orientation disorders as well as cognitive and language dysfunctions. General atrophy of the brain, primarily the hippocampus and frontal cortex, is observed. Microscopically, senile/neuritic plaques composed of, inter alia, β-amyloid concretions, are found. The degeneration of the basal forebrain causes decreased activity of acetylcholine and choline acetyltransferase. Alzheimer’s disease is treated mostly by symptomatic measures since there are no drugs able to slow down or reverse the changes. The main medications include: donepezil, rivastigmine, galantamine and memantine. The prevalence increases from 3% at the age of 65 to 47% at the age of 85. The prognosis is poor; the disease progresses and causes death in a few years, mostly due to complications. Various tests on the effects and potential application of substances of plant origin in treating Alzheimer’s disease have been conducted. Quercetin found in apples, resveratrol in grapes and red wine as well as rosmarinic acid and tanshinones in salvia exhibit a protective action against the toxicity of β-amyloid concretions. Water extract from peach seeds might be a valuable inhibitor of acetylcholinesterase while vegetables possess only anti-butyrylcholinesterase activity. The purpose of this paper is the analysis whether the substances found in plants might be potentially useful in treating Alzheimer’s disease.

PL

Choroba Alzheimera to najczęściej występujące u osób w podeszłym wieku schorzenie neurodegeneracyjne, którego istotą jest postępujące otępienie. U pacjentów obserwuje się upośledzenie pamięci epizodycznej, zaburzenia uwagi, orientacji, funkcji językowych i poznawczych. Stwierdza się uogólniony zanik mózgu, głównie hipokampa i kory czołowej. Mikroskopowo widoczne są płytki starcze, zbudowane m.in. ze złogów β-amyloidu, i zwyrodnienie neurofibrylarne. W wyniku zwyrodnienia podstawnej części przodomózgowia zmniejszają się zawartość acetylocholiny i aktywność acetylotransferazy cholinowej. Postępowanie w przypadku choroby Alzheimera jest głównie objawowe, gdyż nie ma leków hamujących postęp choroby ani odwracających istniejące zmiany. Stosowane są głównie: donepezil, rywastygmina, galantamina i memantyna. Chorobowość zwiększa się z 3% u osób w wieku 65 lat do 47% u osób w wieku 85 lat. Rokowanie jest złe, choroba postępuje, a pacjenci umierają w ciągu kilku lat, głównie z powodu powikłań. Przeprowadzono liczne badania nad działaniem substancji zawartych w roślinach oraz ich potencjalnym wykorzystaniem w terapii choroby Alzheimera. Kwercetyna zawarta w jabłkach, resweratrol obecny w winogronach i czerwonym winie oraz kwas rozmarynowy i tanszinony w szałwii przez różne mechanizmy działają ochronnie wobec toksyczności złogów β-amyloidu. Ekstrakt wodny z pestek brzoskwini zwyczajnej może być użytecznym inhibitorem acetylocholinesterazy, zaś warzywa wykazują jedynie aktywność antybutyrylocholinesterazową. Celem pracy jest przegląd dostępnych badań w poszukiwaniu odpowiedzi na pytanie, czy substancje zawarte w roślinach mogą być stosowane w leczeniu choroby Alzheimera.

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QUERCETIN ATTENUATES OXIDATIVE STRESS, INFLAMMATION AND CARDIAC DYSFUNCTION IN ACRYLAMIDE- INDUCED CARDIOTOXICITY

Antidiabetic cataract effects of GbE, rutin and quercetin are mediated by the inhibition of oxidative stress and polyol pathway

The effect of quercetin on oxidative DNA damage and myelosuppression induced by etoposide in bone marrow cells of rats

Protective effects of quercetin on cadmium fluoride induced oxidative stress at different intervals of time in mouse liver

QUERCETIN AMELIORATES INSULIN RESISTANCE CONCOMITANT EARLY CARDIOVASCULAR CHANGES IN EXPERIMENTAL RATS

Antioxidant Activity and Mechanism in Flower of Hylocereus Undatus (Haw.) Britt. Et Rose

VALIDATION OF HEPATOPROTECTIVE USE OF POLYGONUM PERFOLIATUM EXTRACT AGAINST PARACETAMOL INDUCED TOXICITY IN WISTAR RATS

The antioxidant quercetin protects HL-60 cells with high myeloperoxidase activity against pro-oxidative and apoptotic effects of etoposide

Quercetin influences BSA alpha-helical structures of native, ACR- and NaNO2-modified BSAs

Potencjalne wykorzystanie substancji pochodzenia roślinnego w leczeniu choroby Alzheimera