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1

Characterization of ATPase activity of the AAA ARC from Bifidobacterium longum subsp. infantis

100%

Guzmán-Rodríguez M., de la Rosa A., Santos L.

Acta Biochimica Polonica

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2015

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vol. 62

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issue 2

221-227

EN

Bifidobacteria are considered to be probiotics that exist in the large intestine and are helpful to maintain human health. Oral administration of bifidobacteria may be effective in improving the intestinal flora and environment, stimulating the immune response and possibly preventing cancer. However, for consistent and positive results, further well-controlled studies are urgently needed to describe the basic mechanisms of this microorganism. Analysis of the proteasome-lacking Bifidobacterium longum genome reveals that it possesses a gene, IPR003593 AAA ATPase core, which codes a 56 kDa protein containing one AAA ATPase domain. Phylogenetic classification made by CLANS, positioned this sequence into the ARC divergent branch of the AAA ATPase family of proteins. N-terminal analysis of the sequence indicates this protein is closely related to other ATPases such as the Rhodococcus erythropolis ARC, Archaeoglobus fulgidus PAN, Mycobacterium tuberculosis Mpa and the human proteasomal Rpt1 subunit. This gene was cloned, the full-length recombinant protein was overexpressed in Escherichia coli, purified as a high-molecular size complex and named Bl-ARC. Enzymatic characterization showed that Bl-ARC ATPase is active, Mg+2-dependent and sensitive to N-ethylmaleimide. Gene organization positions bl-arc in a region flanked by a cluster of genes that includes pup, dop and pafA genes. These findings point to a possible function as a chaperone in the degradation pathway via pupylation.

2

The effect of probiotic supplementation as alternative therapy for NAFLD: a literature review

100%

Adnan M. L.

European Journal of Translational and Clinical Medicine

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2021

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vol. 4

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issue 2

75-85

EN

Background: Non-alcoholic fatty liver disease (NAFLD) is one of the most common liver diseases, influenced by various risk factors associated with metabolic disorders. Currently there is no specific effective treatment for NAFLD. Probiotics have been extensively researched for their health benefit Probiotic Supplementation for NAFLD. Methods: A simple literature review was performed based on searches via PubMed, ScienceDirect and Google Scholar, using the keywords "probiotic," "microbiota," "non-alcoholic fatty liver disease," "metabolic disorder," and "therapy." Results: Research on the use of probiotics for NAFLD demonstrated improvement in liver function and histology. However, the literature is inconsistent regarding the probiotics’ influence on the NAFLD risk factors. Probiotics can be an alternative therapy for NAFLD through the ability to modulate the microbiota of the gastrointestinal tract. Conclusion: Probiotics can be an alternative therapy in NAFLD patients, however more extensive studies are needed to ensure the safety of this approach.

3

The impact of polyphenols on Bifidobacterium growth

100%

Gwiazdowska D., Juś K., Jasnowska-Małecka J., Kluczyńska K.

Acta Biochimica Polonica

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2015

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vol. 62

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issue 4

895-901

EN

Polyphenols are a common group of plant based bioactive compounds, that can affect human health because of their antioxidant and antimicrobial properties as well as free-radical scavenging activity. An increasing interest is observed in the interaction between polyphenols and microbiota occurring in food and the human gut. The aim of the work presented here, was to evaluate the effect of some polyphenolic compounds on the growth of two strains of Bifidobacterium: B. adolescentis and B. bifidum. The influence of some flavonoids: naringinin, hesperidin, rutin, quercetin as well as phenolic acids: gallic, caffeic, p-coumaric, ferulic, chlorogenic, vanillic and sinapic was determined by a 96-well microtiter plate assay. In the experiments the effect of three different concentrations of polyphenols: 2, 20 and 100 µg/ml on the growth of Bifidobacterium strains was investigated. All tested compounds influenced the growth of the examined bacteria. Both stimulatory and inhibitory effects were observed in comparison to the positive control. The strongest impact on the growth of bifidobacteria was observed during the first hours of incubation. The constant inhibitory effect was observed for hesperidin and quercetin addition and was dose-dependent. B. bifidum showed a stronger dependence on phenolic acids content in the medium than B. adolescentis during the first hours of incubation.

4

Evaluation of Lactobacillus spp. and yeast based probiotic (Lavipan) supplementation for the reduction of Salmonella Enteritidis after infection of broiler chickens

88%

Smialek M., Kaczorek E., Szczucińska E., Burchardt S., Kowalczyk J., Tykałowski B., Koncicki A., Smialek M., Kaczorek E., Szczucińska E., Burchardt S., Kowalczyk J., Tykałowski B., Koncicki A.

Polish Journal of Veterinary Sciences

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2019

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issue 1

5

Impact of DNA microarray data transformation on gene expression analysis - comparison of two normalization methods

75%

Schmidt M. T., Handschuh L., Zyprych J., Szabelska A., Olejnik-Schmidt A. K., Siatkowski I., Figlerowicz M.

Acta Biochimica Polonica

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2011

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vol. 58

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issue 4

573-580

EN

Two-color DNA microarrays are commonly used for the analysis of global gene expression. They provide information on relative abundance of thousands of mRNAs. However, the generated data need to be normalized to minimize systematic variations so that biologically significant differences can be more easily identified. A large number of normalization procedures have been proposed and many softwares for microarray data analysis are available. Here, we have applied two normalization methods (median and loess) from two packages of microarray data analysis softwares. They were examined using a sample data set. We found that the number of genes identified as differentially expressed varied significantly depending on the method applied. The obtained results, i.e. lists of differentially expressed genes, were consistent only when we used median normalization methods. Loess normalization implemented in the two software packages provided less coherent and for some probes even contradictory results. In general, our results provide an additional piece of evidence that the normalization method can profoundly influence final results of DNA microarray-based analysis. The impact of the normalization method depends greatly on the algorithm employed. Consequently, the normalization procedure must be carefully considered and optimized for each individual data set.

6

Influence of probiotic and yeast culture supplementation on selected biochemical and immunological parameters of growing lambs

75%

Mahmoud M. M., Youssef I. M. I., Abd El-Tawab M. M., Bakr H. A., Eissa N. A., Hassan M. S., Giadinis N. D., Milewski S., Baumgartner W., Sobiech P., Mahmoud M. M., Youssef I. M. I., Abd El-Tawab M. M., Bakr H. A., Eissa N. A., Hassan M. S., Giadinis N. D., Milewski S., Baumgartner W., Sobiech P.

Polish Journal of Veterinary Sciences

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2020

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vol. 23

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issue 1

7

The Effect of Probiotic Addition in Commercial Feed to Growth and Survival Rate of Sangkuriang Catfishes (Clarias gariepinus (Burchell, 1822))

75%

Lili W., Hartanto R., Kurniawati N., Herawati T., Bangkit B. S. I.

World News of Natural Sciences

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2018

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vol. 21

130-140

EN

This study was conducted to determine the effect of probiotic addition in commercial feed to the growth and survival rate of Sangkuriang catfish (Clarias gariepinus). This research was conducted at the Ciparanje Experimental Pond Laboratory, Faculty of Fisheries and Marine Sciences, Universitas Padjadjaran, Jatinangor, West Java. The method in used this research was Completely Randomized Design with four treatments and four replications. The treatment was the variances of probiotic addition which consisted of control (without probiotics), probiotic addition at as much as 0.5 grams / kg of feed, 1 gram / kg of feed and 1.5 grams / kg of feed. The parameters observed were specific growth rate (SGR), absolute biomass, survival rate (SR) and feed conversion ratio (FCR). Data were analyzed using Variant Analysis, at 95% confidence level, and continued with Duncan's Multiple Range Test. The results showed that the increase in the daily growth rate of Sangkuriang catfish was straight-line with the addition of probiotics. Furthermore, commercial probiotics with a dose of 1 gram / kg of feed resulted in specific growth rate of 4.22%, absolute weight of 11 grams, survival rate (SR) of 97.5% and FCR of 0.89.

8

Potential immune modulating properties and antioxidant activity of supplementing commercially available lactoferrin and/orLactobacillus sp. in healthy Ossimi lambs

63%

El-Ashker M., Risha E., Abdelhamid F., Ateya A., El-Ashker M., Risha E., Abdelhamid F., Ateya A.

Polish Journal of Veterinary Sciences

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2018

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vol. 21

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issue 4

9

Antibacterial properties of probiotics bacterial isolated from human breast milk

51%

Blessing E. N., Chukwuemeka I. S., Ukeachu C. D., Onuawuchi U. G.

World News of Natural Sciences

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2020

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vol. 29

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issue 3

290-297

EN

This research work assessed the antibacterial properties of probiotic bacterial which was isolated from breast milk. The breast milk has a distinct amalgamation of minerals, proteins, lipids, carbohydrates and various vitamins that endorse the proper development, growth and immunity of the children. That’s the reason behind it’s consideration to be a comprehensive and inclusive food for new born babies. Furthermore, it is also abundant in various bioactive compounds which encourage the maturation of the immune system over and above develop body’s defense against infections. This research used a standard methodology to isolate the bacterial. In the midst of these bioactive agents, probiotic bacteria were properly isolated from human milk in this research work by means of selective MRS media. Five Lactobacillus spp. were isolated from every one of the three breast milk samples and two Enterococcus species, were observed as potential probiotics, and identified using morphological and biochemical tests which includes L. rhamnosus, L. plantarum, L. casei, L. fermentum, L. acidophilus, En. faecalis and En. faecium. The isolated bacteria were facultative anaerobic, catalase negative, gram positive and non-endospore forming. Sugar fermentation arrangements of equally isolated bacteria also significantly different. The adding up of breast milk probiotics to children formulas possibly will be an innovative substitute to mimic some of the purposeful consequences of human milk in children who are not breastfed.

Refine search results

Characterization of ATPase activity of the AAA ARC from Bifidobacterium longum subsp. infantis

The effect of probiotic supplementation as alternative therapy for NAFLD: a literature review

The impact of polyphenols on Bifidobacterium growth

Evaluation of Lactobacillus spp. and yeast based probiotic (Lavipan) supplementation for the reduction of Salmonella Enteritidis after infection of broiler chickens

Impact of DNA microarray data transformation on gene expression analysis - comparison of two normalization methods

Influence of probiotic and yeast culture supplementation on selected biochemical and immunological parameters of growing lambs

The Effect of Probiotic Addition in Commercial Feed to Growth and Survival Rate of Sangkuriang Catfishes (Clarias gariepinus (Burchell, 1822))

Potential immune modulating properties and antioxidant activity of supplementing commercially available lactoferrin and/orLactobacillus sp. in healthy Ossimi lambs

Antibacterial properties of probiotics bacterial isolated from human breast milk