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1
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Radical scavenging activity of extruded corn gruels with addition of linden inflorescence

100%
Oniszczuk A., Oniszczuk T., Wójtowicz A., Wojtunik K., Kwaśniewska A., Waksmundzka-Hajnos M.
Open Chemistry
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2015
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vol. 13
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issue 1
EN
Antioxidant activity is one of the most desirable properties of natural compounds. Among these substances are phenolic compounds which exhibit excellent antiradical activity. The main aim of the present study was determination of the free radical scavenging activity of gruels with 5, 10 and 20% addition of linden inflorescence. The studies were based on two methods: TLC-bioautographic assay and spectrophotometric analysis using DPPH (2,2-diphenyl-1-picrylhydrazyl radical). The obtained results indicate that the radical scavenging properties of the extracts are positively correlated with the content of phenolic compounds in gruels and that a high-temperature extrusion process does not deactivate antioxidant polyphenolic compounds.
2
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Endophytic colonization by Beauveria bassiana and Metarhizium anisopliae induces growth promotion effect and increases the resistance of cucumber plants against Aphis gossypii

88%
Shaalan R. S., Gerges E., Habib W., Ibrahim L., Shaalan R. S., Gerges E., Habib W., Ibrahim L.
Journal of Plant Protection Research
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2021
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vol. 61
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issue 4
3
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TOTAL PHENOLIC AND FLAVONOID CONTENTS, ANTIOXIDANT AND ANTIBACTERIAL ACTIVITIES OF SELECTED HONEYS AGAINST HUMAN PATHOGENIC BACTERIA

88%
Živković J., Sunarić S., Stanković N., Mihajilov-Krstev T., Spasić A.
Acta Poloniae Pharmaceutica - Drug Research
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2019
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vol. 76
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issue 4
671-681
EN
The study analyzed colour intensity, the total phenolic and flavonoid contents, as well as antioxidant and antibacterial activities of honeys from Serbia. All the tested honeys contained considerable levels of phenolics. The highest phenolic content was obtained in forest honey - 1389.71 mg gallic acid equivalents/kg. High levels of phenolics and flavonoids were also determined in honeydew, meadow and oregano honeys. The radical scavenging activity measured by DPPH method in linden and forest honey was 0.45 and 2.75 mmol Trolox equivalents/kg, respectively. The ferric reducing potential was the greatest in forest honey - 6.04 mmol Fe2+/kg. Darker honeys had a greater phenolic content and antioxidant activity. The antimicrobial activity of honeys against six bacterial strains isolated from human material were studied using a microwell dilution assay. The minimal inhibitory concentration of honeys ranged from 6.25% w/w (for oregano honey against Enterococcus faecalis and honeydew honey against Streptococcus pneumoniae) to >50% w/w (meadow honey against Staphylococcus aureus). The minimal bactericidal concentration ranged from 12.5% w/w (honeydew and bee pollen enriched honey against E. faecalis) to >50% w/w. Higher values of antimicrobial activities were found in honeydew, oregano, and forest honeys, while meadow honey showed the lowest antimicrobial activity. Analyzed honeys exhibited a strong antioxidant and broad-spectrum antibacterial activity against multidrug-resistant pathogenic bacteria. Phenolic compounds, especially in dark amber forest and honeydew honey, as well as in amber oregano honey, may contribute to their efficacy in therapeutic administration.
4
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EVALUATION OF NUTRITIONAL AND MEDICINAL PROPERTIES OF BACOPA MONNIERI BIOMASS AND PREPARATIONS

88%
Muszyńska B., krakowska A., Lazur J., Szewczyk A., Opoka W.
Acta Poloniae Pharmaceutica - Drug Research
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2018
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vol. 75
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issue 6
1353-1361
EN
Bacopa monnieri (Scrophulariaceae) is a well-known plant and has been used by humans for several thousand years. In traditional Hindu medicine, it is one of the most important medicinal plants. The aim of the work was to determine the content of Fe, Mg, and Zn and selected organic compounds before and after extraction into the artificial digestive juices obtained from preparations containing B. monnieri. Commercial preparations in the form of tablets and capsules and B. monnieri shoot cultures were used in the experiment. The metal content in the considered mineralized samples was analyzed by atomic absorption spectrometry and organic compounds by RP-HPLC method. The maximum measured content of the metals released into the digestive juices was as follows: Fe – 32.85; Mg – 367.51; and Zn – 16.41 mg/100 g of the preparation. The existing research shows that metals are best released into the artificial digestive juices from the B. monnieri shoot cultures, and least efficiently from the commercial preparations available in the form of tablets. The phenolic compounds analyzed in the methanol extracts and the extracts of the artificial digestive juices were as follows: protocatechuic acid, p-hydroxybenzoic acid, neochlorogenic acid, chlorogenic acid, isochlorogenic acid, caffeic acid, ferulic acid, cynaroside, trifolin, and luteolin. Bacoside A was only determined in the extracts from the B. monnieri shoot cultures. The experimental results revealed that B. monnieri distributed in the form of tablets did not break down in the artificial digestive juices during the considered time intervals.
5
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RELEASE OF BIOACTIVE SUBSTANCES FROM FORMULATIONS CONTAINING ARTHROSPIRA PLATENSIS (SPIRULINA PLATENSIS)

88%
Muszyńska B., Lazur J., Jękot B., Krakowska A., Szewczyk A., Sułkowska-Ziaja K., Zimmer Ł., Poleszak E., Opoka W.
Acta Poloniae Pharmaceutica - Drug Research
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2018
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vol. 75
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issue 5
1187-1199
EN
Arthrospira platensis (Spirulina platensis) is a well-known microalga and has been utilized as a medicinal agent and foodstuff by humans since at least 16th century. The aim of this study was to determine zinc content as well as determine phenolic and indole compounds from commercial preparations containing Arthrospira platensis (lyophilizate, tablets, and capsules) before and after extraction with methanol and incubation with artificial digestive juices. The secondary aim of this study was to evaluate the quality of these preparations. The samples were incubated in artificial stomach juice and in intestinal juice. The samples were mineralized and their zinc(II) ions content was estimated using flame absorption atomic spectroscopy (F-AAS). The maximum zinc(II) ions content released into the digestive juices was found to be up to 1.6 mg 100 g-1 of the preparation. Phenolic compounds identified in the examined extracts are as follows: gallic acid; protocatechuic acid; 3,4-dihydroxyphenylacetic acid; p-hydroxybenzoic acid; syringe acid; cinnamic acid; and quercetin. Furthermore, indole compounds identified were 5-hydroxy-L-tryptophan, 5-methyl-L-tryptophan, L-tryptophan, tryptamine, and 5-methyltryptamine. Consequently, it was also found that the distributed Arthrospira platensis in the form of tablets does not disintegrate in the artificial digestive juices. Among the examined preparations, only hard capsules met the requirements of the European Pharmacopeia 8th ed.
6
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Phenolic and flavonoid contents in Deschampsia antarctica plants growing in nature and cultured in vitro

75%
Twardovska M., Konvalyuk I., Lystvan K., Andreev I., Parnikoza I., Kunakh V., Twardovska M., Konvalyuk I., Lystvan K., Andreev I., Parnikoza I., Kunakh V.
Polish Polar Research
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2021
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vol. 42
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issue 2
7
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Comparative effects of selected plant polyphenols, gallic acid and epigallocatechin gallate, on matrix metalloproteinases activity in multidrug resistant MCF7/DOX breast cancer cells

75%
Nowakowska A., Tarasiuk J.
Acta Biochimica Polonica
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2016
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vol. 63
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issue 3
571-575
EN
The aim of the study was to investigate the effect of selected polyphenols: gallic acid (GA) and epigallocatechin gallate (EGCG) on matrix metalloproteinase (MMP-2 and MMP-9) activity in multidrug resistant (MDR) human breast adenocarcinoma cells: MCF7/DOX cells and obtained recently in our laboratory MCF7/DOX500 cells by the permanent selection of MCF7/DOX cells with 500 nM doxorubicin (DOX). The activity of MMP-2 and MMP-9 and the effect of studied polyphenols on these matrix proteases were examined by gelatin zymography assays. We have found that the activity of MMP-2 and MMP-9 significantly increased in resistant MCF7/DOX and MCF7/DOX500 cells whereas they were not detected in sensitive MCF7 cells. It was also observed that GA (30, 60, 100 and 120 µM) and EGCG (5, 10 and 20 µM) caused a comparable concentration-dependent inhibition of MMP-2 and MMP-9 activity in MCF7/DOX and MCF7/DOX500 cells. Control experiments confirmed that examined compounds in these ranges of concentration did not affect the cell growth of MCF7/DOX and MCF7/DOX500 sublines (80-100% of control cell growth was observed in the presence of studied polyphenols).
8
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UVB PROTECTIVE, ANTI-AGING, AND ANTI-INFLAMMATORY PROPERTIES OF AQUEOUS EXTRACT OF WALNUT (JUGLANS REGIA L.) SEEDS

75%
Przekora A., Belcarz A., Kowalczyk K., Wójcik M., Wojciechowska K., Ginalska G.
Acta Poloniae Pharmaceutica - Drug Research
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2018
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vol. 75
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issue 5
1167-1176
EN
Walnut (Juglans regia L., fam. Juglandaceae) fruit is found to be very rich in phenolic compounds and thus to show wide spectrum of biological activities like antioxidant, anti-inflammatory, or antitumour properties. Ethanol or methanol are preferentially used for preparation of walnut extracts for cosmetic applications. However, it is commonly known that alcohol causes dehydration and redness of the skin. The aim of this work was to prepare aqueous extract of undamaged walnut seeds rich in pellicles and to evaluate its antioxidant, anti-apoptotic, anti-inflammatory, and anti-aging properties in vitro. Conducted research clearly demonstrated that simple water extraction of undamaged kernels with pellicles allows to obtain rich in phenolic compounds (36-38 mg per g of lyophilisate) walnut extract, which protects fibroblasts and keratinocytes against oxidative stress induced by UVB dose of 35 mJ/cm2 (5-6-h exposure on a sunny day), protects keratinocytes against UVB-induced apoptotic death, limits the development of the inflammation in epidermis, and also possesses ability to inhibit collagenase and elastase. Thus, obtained aqueous walnut extract (at relatively low concentration of 5 µg/ml) is found to be a promising compound of sunscreen and anti-aging cosmetic formulations.
9
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Allelopathic efficiency of Eruca sativa in controlling two weeds associated with Pisum sativum plants

75%
El-Wakeel M. A., Ahmed S. E.-D. A. E.-G., El-Desoki E. R.
Journal of Plant Protection Research
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2019
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vol. 59
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issue 2
10
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Dpph Radical Scavenging Activity and Phenolic Compound Content in Different Leaf Extracts from Selected Blackberry Species

63%
Gawron-Gzella A., Dudek-Makuch M., Matławska I.
Acta Biologica Cracoviensia s. Botanica
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2012
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vol. 54
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issue 2
11
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Sex-related differences in the dioecious species Rumex thyrsiflorus Fingerh. – analysis of the content of phenolic constituents in leaf extracts

63%
Dziedzic K., Szopa A., Waligórski P., Ekiert H., Ślesak H., Dziedzic K., Szopa A., Waligórski P., Ekiert H., Ślesak H.
Acta Biologica Cracoviensia s. Botanica
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2020
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vol. 62
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issue 1
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