Search results

1

Supported liquid membrane extraction of peptides.

100%

Drapała A., Dżygiel P., Jönsson J. Å., Wieczorek P.

Acta Biochimica Polonica

|

2001

|

vol. 48

|

issue 4

1113-1116

EN

The application of supported liquid membrane (SLM) extraction for the enrichment of short peptides is presented. The extraction efficiency is dependent on the pH of donor phase and salt concentration in acceptor phase. Moreover, the extraction efficiency is also influenced by the peptide amino-acid sequence and hydrophobicity.

2

ATP-binding peptide-hydrogel composite synthesized by molecular imprinting on beads

100%

Takata A., Usui K., Matsui J.

Molecular Imprinting

|

2014

|

vol. 2

|

issue 1

EN

Molecular imprinting has been recognized as a useful technique to produce synthetic mimics of functional proteins, such as antibodies and enzymes. However, only a few studies have examined peptides as starting materials for synthesizing molecularly imprinted polymers in spite of the expectation that peptides would be suitable materials for realizing water-compatibility and proteinlike functions. In this study, molecular imprinting was performed using a vinyl-end-capped on-beads-peptide as functional monomer to produce an on-beads-peptide hydrogel composite selective for ATP; the on-beadspeptide peptide, of which sequence was designed to possess both an adenine-recognition site and phosphate recognition site, was co-polymerized with NIPAM and BIS in the presence of ATP as a template species. The resultant ATP-imprinted composite showed 14-times higher affinity and an enhanced selectivity towards ATP, suggesting that the peptide conformation, i.e. a mutual orientation of the two binding sites, was pre-organized and immobilized in a manner where the ATP binding is more favored.

3

Free energy of helix propagation in short polyalanine chains determined from peptide growth simulations of La3+-binding model peptides. Comparison with experimental data

88%

Maciejczyk M., Hermans J., Bierzyński A.

Acta Biochimica Polonica

|

2006

|

vol. 53

|

issue 1

121-130

EN

Molecular dynamics (MD) is, at present, a unique tool making it possible to study, at the atomic level, conformational transitions in peptides and proteins. Nevertheless, because MD calculations are always based on a more or less approximate physical model, using a set of approximate parameters, their reliability must be tested by comparison with experimental data. Unfortunately, it is very difficult to find a peptide system in which conformational transitions can be studied both experimentally and using MD simulations so that a direct comparison of the results obtained in both ways could be made. Such a system, containing a rigid α-helix nucleus stabilized by La3+ coordination to a 12-residue sequence taken from an EF-hand protein has recently been used to determine experimentally the helix propagation parameters in very short polyalanine segments (Goch et al. (2003) Biochemistry 42: 6840-6847). The same parameters were calculated here for the same peptide system using the peptide growth simulation method with, alternatively, charmm 22 and cedar potential energy functions. The calculated free energies of the helix-coil transition are about two times too large for cedar and even three times too large for charmm 22, as compared with the experimental values. We suggest that these discrepancies have their origin in the incorrect representation of unfolded peptide backbone in solution by the molecular mechanics force fields.

Refine search results

2 Acta Biochimica Polonica

1 Molecular Imprinting

1 2016

1 2006

1 2001

1 Bierzyński A.

1 Drapała A.

1 Dżygiel P.

1 Hermans J.

1 Jönsson J. Å.

1 Maciejczyk M.

1 Matsui J.

1 Takata A.

1 Usui K.

1 Wieczorek P.

Supported liquid membrane extraction of peptides.

ATP-binding peptide-hydrogel composite synthesized by molecular imprinting on beads

Free energy of helix propagation in short polyalanine chains determined from peptide growth simulations of La3+-binding model peptides. Comparison with experimental data