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EN
The purpose of this study was to evaluate the analgesic, phytochemical screening, acute toxicity, and moisture content activity of the crude ethanolic seed extract of Cuminum cyminum and its n-hexane, chloroform, carbon tetrachloride and aqueous fraction. The ethanolic extract was used to the moisture content was found 43% evaluate analgesic, phytochemical screening, and moisture content activities. The analgesic activity was measured by acetic acid induced writhing test. The extract showed highly significant (p <0.001) analgesic activity with % inhibitions of writhing response at doses 250 mg/kg was 24.67% and 500 mg/kg was 36.95%. The moisture content was found to be 43%. The results of the study clearly indicate the present of analgesic, phytochemical screening and moisture content properties of the extract and its fractions. Phytochemical analysis revealed the presence of alkaloids, saponins, phenols, terpenoids and glycosides. Screening of analgesic property on mice by acetic acid induced method for crude extracts and its different fractions of Cuminum cyminum. At zero-hour test samples, control (1% Tween-80 solution in saline) and Diclofenac sodium were administered orally by means of a long needle with a ball shaped end. After 40 minutes acetic acid (1%) was administered intra- peritoneally to each of the animals of all the groups. The forty minutes interval between the oral administration of test materials and intra-peritoneal administration of acetic acid was given to assure proper absorption of the administered samples. Five minutes after the administration of acetic acid, number of squirms or writhing were counted for each mouse for ten minutes. Each mouse of all groups was observed individually for counting the number of writhing they made in 15 minutes commencing just 5 minutes after the Intra-peritoneal administration of acetic acid solution. Full writhing was not always accomplished by the animal, because sometimes the animals started to give writhing but they did not complete it. This incomplete writhing was considered as half-writhing. Accordingly, two half writhing were taken as one full writhing. In conclusion the ethanolic extract of C. cyminum seeds showed predominately showed significant analgesic activity.
EN
Polygalacturonase (PG) production by Penicillium chrysogenum during solid-state fermentation was accompanied by decomposition of orange peels. A leaching procedure was developed through the selection of solvent, time and intensity of stirring. A maximum PG activity was observed after 48 h peel inoculation. Further cultivation decreased the enzyme activity significantly, up to 60% of the maximum PG activity. During fermentation, a rapid acidification of the solid medium which inhibited the pectinolytic enzyme, was observed. Buffering agents with different pH values and different ionic strengths were examined to identify the most suitable medium to avoid this problem. Buffer addition counteracted acidification and enhanced active protein production, which was observed for all of the applied pH values (6.5-8.0) of the buffering agent. The most satisfactory results were obtained when using the highest pH at 8.0. The protein content and PG activity increased from 3.5 mg/g and 1.09 U/g to 7.7 mg/g and 7.11 U/g during cultivation, with uncontrolled and pH-controlled medium, respectively. Measurements at wide pH and temperature ranges indicated an optimum for PG activity at pH 5.0 and 43°C; however, high thermal stability corresponded to lower temperatures, and a temperature of 37°C is thus recommended. Under these conditions, the operational stability was determined to be t1/2=570 h.
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2020
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vol. 29
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issue 3
162-178
EN
Technical information on lumber seasoning, moisture content (MC), density, mechanical, workability and chemical characteristics would strongly determine rational utilization of each lumber species. A study was conducted on home-grown two Eucalyptus species (Eucalyptus pilularis and Eucalyptus viminalis) and one indigenous (Trichilia dregeana) timbers with the main objective of determining some characteristics of lumber that will indicate quality and proper utilization. Study species were harvested from Shashemene, Asella and Arjo sites, respectively. The experimental design for seasoning and density was complete randomized design, a factorial experiment. The experiments were conducted using air and kiln seasoning methods. To determine initial moisture content oven drying method was used. The mean initial MC for the three timber species were 48.25%, 56.1% and 65.45%, respectively. The species were classified as very rapid seasoning rate during kiln seasoning. The mean shrinkage values were Tangential (6.31, 4.94 and 4.43%), radial (3.4, 4.02 and 2.10%) volumetric (9.46, 8.6 and 6.37%), when they seasoned from green to 12% MC, respectively. Seasoning defects such as cup, bow, crook, end split, surface and end-checks were observed, though the extent varies with species. The density of E. pilulalris, E. viminalis and T. dregeana at 12% MC was 780, 810 and 530 Kg/m3, respectively. The one-way analysis of variance indicated that there was significant difference (P>0.001) in initial MC, final MC, density values at different MC and shrinkage characteristics. They revealed good lumber characteristics and qualities, comparable with many indigenous and home-grown exotic timber species in density, seasoning rate and shrinkage. The tree species have to be well managed, properly harvested and sawn. Boards have to be properly stacked and seasoned to about 12% MC, with kiln and air seasoning methods that can help to minimize seasoning time, seasoning defects, shrinkage characteristics and increase quality.
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