Search results

1

Molecular cloning and expression analysis of a new gene for short-chain dehydrogenase/reductase 9

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Liu S., Huang C., Li D., Ren W., Zhang H., Qi M., Li X., Yu L.

Acta Biochimica Polonica

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2007

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vol. 54

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issue 1

213-218

EN

We report here the cloning and characterization of a novel human short-chain dehydrogenases/reductase gene SCDR9, isolated from a human liver cDNA library, and mapped to 4q22.1 by browsing the UCSC genomic database. SCDR9 containing an ORF with a length of 900 bp, encoding a protein with a signal peptide sequence and an adh_short domain. GFP localization shows SCDR9 protein concentrated in some site of the cytoplasm, but not in the ER. Expression pattern in eighteen tissues revealed that SCDR9 is expressed highly in liver. Soluble recombinant protein was successfully purified from Escherichia coli using pET28A(+) expression vector. Our data provides important information for further study of the function of the SCDR9 gene and its products.

2

Synchronous occurrence of multiple focal nodular hyperplasia and huge hepatic Perivascular epithelioid cells tumor (PEComa) in young woman after oral contraceptive use – is there a common pathogenesis?

100%

Durczyński A., Hogendorf P., Szymański D., Sporny S., Strzelczyk J.

Polish Journal of Surgery

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2012

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vol. 84

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issue 9

457-460

EN

The association of focal nodular hyperplasia (FNH) and various neoplasms was described, but coincidence of multiple FNH and hepatic perivascular epithelioid cells tumor (PEComa) has not been reported. The clinical debate of oral contraceptive (OC) influence on FNH growth is ongoing, but no evidence exists about association of hepatic PEComa with OC use. Herein, we report a case of two FNH lesions and huge (150x100x80 mm) left hepatic lobe PEComa that occurred simultaneously in 18-year-old female with previous two year history of OC use, who underwent left hemihepatectomy and right hepatic FNH enucleation. Up to date, the patient has been followed-up for 65 months and remained disease-free. FNH and PEComa have a common vascular cytogenetic denominator. Our case raising a question of a causal relationship of FNH and PEComa with OC use that might be attributed to vascular changes. Future researches of larger sample sizes should further address this issue.

3

An unusual case of a tuberculous granuloma of the liver presenting thirteen years after intravesical BCG – therapy for bladder cancer

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Szczęsny W., Szylberg Ł., Słupski M., Marszałek A., Szmytkowski J.

Polish Journal of Surgery

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2021

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vol. 93

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issue 5

1-5

EN

The authors present the case of a female patient with a tumor of segment VII of the liver, which was postoperatively identified as a tuberculous granuloma. The patient was admitted for elective surgery for a liver tumor, which had been diagnosed a few months before. Computed tomography and nuclear magnetic resonance were performed, based on which focal nodular hyperplasia was suspected. Thirteen years prior to admission the patient had undergone a transurethral resection of superficial bladder carcinoma, followed by adjuvant intravesical Bacillus Calmette-Guérin (BCG-therapy). Upon surgery, segment VII of the liver was resected; postoperative course was uneventful. After the identification of granuloma, the patient was referred to a phthysiatric clinic for further diagnostics and treatment. The authors have deemed this case worthy of reporting primarily due to the exceptionally long period between the completion of BCG therapy and the onset of hepatic tumor.

4

Investigation of the hepatoprotective properties of “Phytovenol” capsules on the model of medicamentous hepatitis in rats

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Gerush O., Lenytska O., Yakovleva L., Gladkova L., Gerush I.

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vol. 27

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issue 4

240-242

EN

This scientific paper is an investigation of the pharmacological activity of the new medical preparation “Phytovenol” (capsules in the dose of 150 mg/kg) on the pattern of hepatitis in rats induced by paracetamol administration. The authors of the article, for the first time under experimental conditions, detected a hepatoprotective effect that was brought about by “Phytovenol” capsule administration. This is realized due to the antioxidant, antitoxic and anticytolytic activity of the examined medicine. The study found that its efficacy is not lower than the classical hepatoprotector - “Silibor” tablets. The results obtained suggest that it is possible to extend the range of available hepatoprotectors and improve the quality of treatment for liver diseases, by introducing “Phytovenol” capsules into medical practice.

5

Cartilaginous compression of the liver – clinical and ultrasonographic aspects

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Smereczyński A.

Journal of Ultrasonography

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2018

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vol. 18

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issue 72

9-15

EN

Introduction: The musculocartilaginous complex is a structure composed of cartilaginous, osseous and muscular elements, which is located at the thoracoabdominal junction, at the level of the right costal arch. Aim: To determine the ultrasonographic characteristics of this complex under normal conditions as well as to demonstrate its effects on the liver depending on the constitutional body built, respiratory phase and patient’s body position. Materials and methods: All abdominal ultrasound scans were performed between 2006 and 2015. A total of 1000 patients (566 females and 434 males aged between 35 and 82 years, mean age 52 years), who had no significant upper abdominal pathologies identified based on clinical and imaging data, were enrolled for the analysis. In addition to standard internal organ assessment, we also attempted to identify the symptoms of hepatic compression by the musculocartilaginous complex. We used 3–6 MHz convex and 7–12 MHz linear transducers. The degree of musculocartilaginous compression of the liver was assessed during breathing in supine and sitting position, as well as with trunk inclined forward. Results: The study showed that musculocartilaginous compression of the liver mostly affects females (96%) with leptosomatic body build. The complex compressing the liver shows a heterogeneous echostructure. Increased hepatic compression was observed during exhalation, in a sitting position and with trunk inclined forward. Conclusions: Cartilaginous compression of the liver depends on body built and patient’s body position. The musculocartilaginous complex may cause focal or segmental compression of the hepatic parenchyma, causing pain in the right upper abdomen in some patients.

PL

Wprowadzenie: Kompleks chrząstkowo-mięśniowy to struktura składająca się z elementów chrzęstnych, kostnych i mięśniowych, zlokalizowana na pograniczu piersiowo-brzusznym, na poziomie prawego łuku żebrowego. Cel: Określenie ultrasonograficznych cech tego kompleksu w warunkach prawidłowych oraz wykazanie jego wpływu na wątrobę w zależności od budowy konstytucjonalnej, fazy oddechowej oraz pozycji przyjmowanej przez pacjenta. Materiał i metoda: Wszystkie badania ultrasonograficzne jamy brzusznej przeprowadzono w okresie od 2006 do 2015. Do analizy zakwalifikowano 1000 osób (566 kobiet i 434 mężczyzn, w przedziale wiekowym 35–82 lata, średnio 52 lata), u których na podstawie danych klinicznych i obrazowych nie stwierdzono istotnych zmian w narządach nadbrzusza. W badaniach ultrasonograficznych jamy brzusznej, oprócz standardowo ocenianych narządów wewnętrznych, skupiono się na poszukiwaniu objawów ucisku wątroby przez kompleks chrząstkowo-mięśniowy. Do badań wykorzystywano głowice konweksowe o częstotliwości 3–6 MHz oraz liniowe o częstotliwości 7–12 MHz. Stopień ucisku wątroby przez kompleks chrząstkowo-mięśniowy oceniano w trakcie oddychania, w ułożeniu pacjenta na plecach, w pozycji siedzącej i w przodopochyleniu tułowia. Wyniki: Wykazano, że ucisk chrząstkowopochodny wątroby dotyczy głównie kobiet (96%) o budowie leptosomicznej. Uciskający wątrobę kompleks najczęściej wykazuje heterogeniczną echostrukturę. Większy stopień ucisku wątroby występował w trakcie wydechu, w pozycji siedzącej i w przodopochyleniu tułowia. Wnioski: Ucisk chrząstkowopochodny wątroby jest uzależniony od budowy ciała oraz przyjmowanej przez pacjenta pozycji. Kompleks chrząstkowo-mięśniowy może wywierać ucisk ogniskowy lub odcinkowy na miąższ wątroby, powodując u części osób ból w prawym nadbrzuszu. Artykuł w wersji polskojęzycznej jest dostępny na stronie http://jultrason.pl/index.php/wydawnictwa/volume-18-no-72

6

Detailed EFSUM recommendations on the scope of ultrasound assessment in patients with portal hypertension considering the diagnostic reference level

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Smereczyński A., Kołaczyk K.

Journal of Ultrasonography

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2017

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vol. 17

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issue 69

113-115

EN

An important paper describing the Standards of the Polish Ultrasound Society regarding the assessment of portal and hepatic vasculature was published in the Journal of Ultrasonography. Due to the multiplicity of morphological and hemodynamic data required, the time needed to obtain these data and the legal responsibility of doctors for the results, there seems to be a need to determine a clear range of the assessed parameters depending on the reference level of a given healthcare facility. Therefore, the aim of the paper was to present the EFSUMB recommendations on the range of the evaluated ultrasonographic parameters in portal hypertension depending on the reference level. European healthcare institutions are characterized by a clear three-level reference network. Due to the lack of a similar division in Poland, we propose our own classification of the competence of medical entities. The first reference level: ultrasound assessments in a primary health care setting (performed by GPs, emergency physicians, non-specialist private practice physicians, non-specialist practice physicians); at least one mid-class ultrasound scanner with pulsed and color Doppler options, equipped with convex 3–5 MHz and linear 7–12 MHz transducers should be available at physician’s disposal. The second reference level: ultrasound assessments in the hospital setting and specialist outpatient clinics, performed by specialist private practice physicians, radiologists, gastroenterologists and hepatologists; top class (premium) digital ultrasound scanner should be available at physician’s disposal. Third reference level: ultrasound assessments performed in gastroenterology, hepatology and liver surgery departments as well as their specialist outpatient clinics; physicians should use top class digital ultrasound equipment. At every reference level, physicians performing abdominal ultrasound should have the appropriate certification to perform such an assessment or specialize in gastrointestinal diagnosis.

PL

W „Journal of Ultrasonography” została opublikowana ważna praca opisująca standardy badań ultrasonograficznych Polskiego Towarzystwa Ultrasonograficznego dotyczące oceny układu wrotnego i naczyń wątroby. Z uwagi na mnogość koniecznych do określenia danych morfologicznych i hemodynamicznych, czas potrzebny do ich uzyskania oraz odpowiedzialność prawną lekarzy za ich wynik wydaje się, że konieczne jest sporządzenie dokładnego zakresu ocenianych parametrów w zależności od referencyjności ośrodka. Celem pracy jest więc zaprezentowanie rekomendacji Europejskiej Federacji Towarzystw Ultrasonografii w Medycynie i Biologii określających zakres badanych parametrów ultrasonograficznych dotyczących nadciśnienia wrotnego w odniesieniu do referencyjności ośrodka. W wielu krajach europejskich istnieje wyraźna trójstopniowa referencyjność ośrodków medycznych. W związku z tym, że w Polsce taki podział nie obowiązuje, autorzy tej pracy proponują własną klasyfikację kompetencyjności podmiotów medycznych. I stopień referencyjności: badania USG wykonywane w ramach podstawowej opieki medycznej (przez lekarza rodzinnego, lekarza SOR, lekarza zajmującego się niespecjalistyczną praktyką prywatną, lekarza pracującego w przychodni niespecjalistycznej); do dyspozycji lekarza powinien być co najmniej aparat cyfrowy klasy średniej z opcją impulsowego i kolorowego dopplera oraz dopplera mocy, wyposażony w głowicę konweksową 3–5 MHz i liniową 7–12 MHz. II stopień referencyjności: badania wykonywane w warunkach szpitalnych, w przychodniach specjalistycznych, przez lekarzy wykonujących specjalistyczną praktykę prywatną oraz przez radiologów, gastrologów i hepatologów; do dyspozycji lekarza powinien być aparat cyfrowy najwyższej klasy (premium). III stopień referencyjności: badania ultrasonograficzne wykonywane w klinikach o profilu gastrologicznym, hepatologicznym i chirurgii wątroby oraz w przychodniach specjalistycznych tych placówek; lekarz powinien badać na aparacie cyfrowym najwyższej klasy. Na każdym etapie ośrodka referencyjnego lekarz wykonujący badania USG jamy brzusznej powinien legitymować się odpowiednim certyfikatem uprawniającym do wykonywania takich badań lub specjalizacją z zakresu diagnostyki przewodu pokarmowego. Artykuł w wersji polskojęzycznej jest dostępny na stronie http://jultrason.pl/index.php/wydawnictwa/volume-17-no-69

7

Methylation demand: a key determinant of homocysteine metabolism.

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Brosnan J. T., Jacobs R. L., Stead L. M., Brosnan M. E.

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EN

Elevated plasma homocysteine is a risk factor for cardiovascular disease and Alzheimer's disease. To understand the factors that determine the plasma homocysteine level it is necessary to appreciate the processes that produce homocysteine and those that remove it. Homocysteine is produced as a result of methylation reactions. Of the many methyltransferases, two are, normally, of the greatest quantitative importance. These are guanidinoacetate methyltransferase (that produces creatine) and phosphatidylethanolamine N-methyltransferase (that produces phosphatidylcholine). In addition, methylation of DOPA in patients with Parkinson's disease leads to increased homocysteine production. Homocysteine is removed either by its irreversible conversion to cysteine (transsulfuration) or by remethylation to methionine. There are two separate remethylation reactions, catalyzed by betaine:homocysteine methyltransferase and methionine synthase, respectively. The reactions that remove homocysteine are very sensitive to B vitamin status as both the transsulfuration enzymes contain pyridoxal phosphate, while methionine synthase contains cobalamin and receives its methyl group from the folic acid one-carbon pool. There are also important genetic influences on homocysteine metabolism.

8

The influence of single application of paracetamol and/or N-acetylcysteine on rats in subchronic exposition to trichloroethylene vapours. II. Effect on hepatic glutathione level

88%

Plewka D., Plewka A., Kowalówka-Zawieja J., Zielińska-Psuja B., Przystanowicz J.

Medycyna Środowiskowa - Environmental Medicine

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2012

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vol. 15

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issue 3

91-95

EN

Background: Feature of modern existing hazards both environmental and occupational is cumulative exposure often leading to unexpected response of the organism resulting, among other things, in interactions with cytochrome P450 system involved in biotransformation of trichloroethylene and paracetamol. Hepatotoxity of paracetamol is closely connected with hepatic glutathione level. „In therapy of acute paracetamol poisoning application of N-acetylcysteine as a factor, which protects GSH level in cells, is recommended.” Materials and method: Tests were performed on rats which were treated with trichloroethylene, paracetamol and/or N-acetylcysteine. In rat liver total level of glutathione was determined i.e. reduced and oxidized form. Results: Paracetamol just after completion of the exposure affected the glutathione level. Trichloroethylene throughout the period of observation stimulated growth of glutathione level in liver. N-acetylcysteine didn’t have any influence on the level of investigated tripeptyde. Conclusions: N-acetylcysteine removes negative effect of paracetamol especially when it’s applied with 2-hour delay. After exposure for trichloethylene immediate application of N-acetylcysteine caused noticeable lowering of glutathione level. Cumulative exposure for three xenobiotics had positive influence for glutathione level in rat liver.

PL

Wstęp: Cechą współcześnie występujących zagrożeń, zarówno środowiskowych jak i zawodowych jest narażenie łączne, wielokrotnie prowadzące do nieprzewidzianej odpowiedzi biologicznej organizmu, wynikającej między innymi z oddziaływań na układ cytochromu P450 biorący udział w biotransformacji trichloroetylenu i paracetamolu. Hepatotoksyczność paracetamolu jest między innymi ściśle związana z wątrobowym poziomem glutationu. W terapii zatruć ostrych paracetamolem zalecane jest podawanie N-acetylocysteiny jako czynnika ochraniającego poziom GSH w komórkach. Materiał i metody: Badania wykonano na szczurach, które traktowano trichloroetylenem, paracetamolem i/lub N-acetylocysteiną. W wątrobie szczura oznaczano poziom całkowity glutationu, tj. formę zredukowaną i utlenioną. Wyniki: Paracetamol tuż po zakończeniu ekspozycji negatywnie wpływał na poziom glutationu. Trichloroetylen przez cały czas obserwacji stymulował wzrost poziomu glutationu w wątrobie. N-acetylocysteina nie miała żadnego wpływu na poziom badanego tripeptydu. Wnioski: N-acetylocysteina usuwała negatywny wpływ paracetamolu, szczególnie wtedy, kiedy podano ją z 2-godzinnym opóźnieniem. Po narażeniu na trichloroetylen natychmiastowe podanie N-acetylocysteiny niosło za sobą wyraźnie obniżenie poziomu glutationu. Narażenie łączne na trzy oceniane ksenobiotyki.

9

The influence of single application of paracetamol and/or N-acetylcysteine on rats in subchronic exposition to trichloroethylene vapours. III. Effect on some isoforms of cytochrome P450 in liver

88%

Plewka A., Plewka D., Kowalówka-Zawieja J., Przystanowicz J., Zielińska-Psuja B.

Medycyna Środowiskowa - Environmental Medicine

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2012

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vol. 15

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issue 4

24-30

EN

Background: In case of overdose of paracetamol the ability of hepatic biotransformation is saturated and accumulation of toxic metabolite – NAPQI takes place. Main CYP isoforms considered to be responsible for bioactivation of APAP and promoting the same liver intoxication are CYP2E1, CYP1A2, CYP3A4 and in animals 2B1/2 isoforms additionally. Purpose of this work was examination of paracetamol influence and/or trichloroethylene on the composition of hepatic cytochrome P450 isoforms. Materials and method: Tests were carried out on rats which were treated with trichloroethylene, paracetamol and/or N-acetylcysteine. In the microsomal fraction content of three isoforms of cytochrome P450 i.e. CYP2E1, CYP2B1/2 and CYP1A2 were determined. Results: Paracetamol slightly stimulated CYP2B1/2 lowering simultaneously level of CYP1A2. Trichloroethylene stimulated CYP2B1/2. N-acetylcysteine stimulated all tested P450 isoforms. N-acetylcysteine given together with examinated xenobiotics induced studied P450 isoforms. Conclusions: N-acetylcysteine demonstrated a protective effect on studied CYP isoforms especially when was given upon termination of xenobiotics exposure.

PL

Wstęp: W przypadku przedawkowania paracetamolu, zdolność wątroby do detoksykacji zostaje wysycona i następuje akumulacja toksycznego metabolitu, jaki jest NAPQI. Główne izoformy CYP, uważane za odpowiedzialne za bioaktywację APAP-u i sprzyjające w ten sposób zatruciom wątrobowym, to CYP2E1, CYP1A2 oraz CYP3A4 a u zwierząt dodatkowo izoformy 2B1/2. Celem pracy było zbadanie wpływu paracetamolu i/lub trichloroetylenu na skład wątrobowych izoform cytochromu P450. Materiał i metody: Badania wykonano na szczurach, które traktowano trichloroetylenem, paracetamolem i/lub N-acetylocysteiną. We frakcji mikrosomalnej wątroby oznaczano zawartość trzech izoform cytochromu P450, tj., CYP2E1, CYP2B1/2 oraz CYP1A2. Wyniki: Paracetamol lekko stymulował CYP2E1 obniżając równocześnie poziom CYP1A2. Trichloroetylen stymulował CYP2B1/2. N-acetylocysteina miała stymulujący wpływ na wszystkie badane izoformy P450. N-acetylocysteina podawana łącznie z badanymi ksenobiotykami prowadziła do wyraźnych wzrostów CYP. Wnioski: N-acetylocysteina wykazywała ochronny wpływ na poziomy badanych izoform cytochromu P450, szczególnie, jeśli została podana zaraz po zaprzestaniu ekspozycji na ksenobiotyki.

10

Silver (Ag) in tissues and eggshells, biochemical parameters and oxidative stress in chickens

88%

Chmielowiec-Korzeniowska A., Tymczyna L., Dobrowolska M., Banach M., Nowakowicz-Dębek B., Bryl M., Drabik A., Tymczyna-Sobotka M., Kolejko M.

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issue 1

EN

This study aimed to assess levels of silver nanoparticle residues in eggshells and tissues as well as the levels of selected biochemical parameters and oxidative stress indices in chickens hatched from nanosilver disinfected eggs. The samples included 40 Greenleg Partridge chicks allocated into two groups. The experimental group (group D) consisted of chickens hatched from eggs disinfected with a nanosilver preparation prior to incubation, while the control group (group C) included chickens whose eggs were exposed to UV radiation for disinfection. The eggshells and kidney sections obtained from group D chickens showed a significantly higher silver level compared to group C. For the biochemical parameters, only the uric acid content was higher in group D compared to group C. Analysis of the antioxidative stress biomarkers (superoxide dismutase and catalase), showed a significant increase in group D in relation to group C.

11

The influence of single application of paracetamol and/or N-acetylcysteine on rats subchronic exposed to trichloroethylene vapours. I. Effect on hepatic moonooxygenase system dependent of cytochrome P450

88%

Plewka A., Plewka D., Przystanowicz J., Kowalówka-Zawieja J., Zielińska-Psuja B.

Medycyna Środowiskowa - Environmental Medicine

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2012

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vol. 15

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issue 2

64-71

EN

Background: There is a number of factors which potentially affect occurrence of toxic change in liver after overdosing of paracetamol. Hepatic metabolism of trichloroethylene has primary impact on hepatotoxic effect of this solvent. This means that the combined exposure to these xenobiotics can be particularly harmful for human. The influence of N-acetylcysteine (NAC) as a protective factor after paracetamol intoxication was studies. Materials and method: Tests were carried out on rats which were treated with trichloroethylene, paracetamol and/or N-acetylcysteine. In the hepatic microsomal fraction activity of the components of cytochrome P450- dependent monooxygenases was determined Results: Paracetamol slightly stimulated cytochrome P450 having no effect on reductase activity cooperating with it. Cytochrome b5 and its reductase were inhibited by this compound. Trichloroethylene was the inhibitor of compounds of II microsomal electron transport chain. N-acetylcysteine inhibited activity of reductase of NADH-cytochrome b5. Conclusions: Tested doses of the xenobiotics influenced on II microsomal electron transport chain. Protective influence of N-acetylcysteine was better if this compound was applied 2 hours after exposure on xenobiotics.

PL

Wstęp: Istnieje szereg czynników, które potencjalnie wpływają na ryzyko wystąpienia zmian toksycznych w wątrobie po przedawkowaniu paracetamolu. Wątrobowy metabolizm trichloroetylenu ma pierwszorzędny wpływ na hepatotoksyczny efekt tego rozpuszczalnika. Oznacza to, że narażenie łączne na te ksenobiotyki może być szczególnie szkodliwe dla człowieka. Oceniono wpływ N-acetylocysteiny (NAC) jako czynnika osłaniającego po zatruciu paracetamolem. Materiał i metody: Badania wykonano na szczurach, które traktowano trichloroetylenem, paracetamolem i/lub N-acetylocysteiną. We frakcji mikrosomalnej wątroby oznaczano aktywność składników monooksygenaz zależnych od cytochromu P450. Wyniki: Paracetamol lekko stymulował cytochrom P450 nie mając wpływu na aktywność reduktazy współpracującej z nim. Cytochrom b5 i jego reduktaza były hamowane przez ten związek. Trichloroetylen był inhibitorem składników II mikrosomalnego łańcucha transportu elektronów. N-acetylocysteina hamowała aktywność reduktazy NADH-cytochrom b5. Wnioski: Badane dawki ocenianych ksenobiotyków swój wpływ ujawniały raczej na składniki II mikrosomalnego łańcucha transportu elektronów. Ochronny wpływ N-acetylocysteiny był wyraźniejszy, jeśli podano ten związek 2 godziny po zakończeniu ekspozycji na badane ksenobiotyki.

12

The effects of galactosamine on UTP levels in the livers of young, adult and old rats.

88%

Kmieć Z., Smoleński R. T., Zych M., Myśliwski A.

Acta Biochimica Polonica

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2000

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vol. 47

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issue 2

349-353

EN

Galactosamine (GalN), a well-known hepatotoxin that depletes the cellular pool of uracil nucleotides, was previously shown to have greater impact on the inhibition of protein synthesis in hepatocytes of old rats as compared with young animals (Kmieć 1994, Ann. N.Y. Ac. Sci. 717, 216-225). In the present study we compared the effects of GalN on the nucleotide content (measured by ion-exchange HPLC) in the livers of young (4 months), adult (12 months), and old (24-26 months old) rats two hours after its intraperitoneal administration. UTP content of the livers of old control rats was significantly lower (by 28%) than that of young animals. GalN administration decreased the UTP content in the livers of young, adult and old rats by, respectively, 55%, 65% and 89%, and increased the content of UDP-sugars by 189%, 175% and 305%. The hepatic content of ATP, ADP, AMP, NAD, GTP except CTP did not differ significantly among the age groups of rats studied, and was not changed by GalN treatment. The content of CTP was significantly higher in old rats (P < 0.03) upon GalN treatment. The lower hepatic content of UTP may partially explain the increased sensitivity of hepatocytes and livers of old rats to the action of galactosamine, and possibly to other hepatotoxic compounds that decrease transcription in the liver.

13

The effect of one-electron reduced drugs on hepatic aconitase activity and triglycerides level

88%

Wnukowska M., Mandziuk S., Korga A., Jodlowska-Jedrych B., Matysiak W., Halasa J., Burdan F., Iwan M., Gieroba R., Dudka J.

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2015

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vol. 28

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issue 1

5-7

EN

The redox cycle triggered by one electron reduction of doxorubicin and tirapazamine - both anticancer agents - leads to superoxide production. This superoxide production itself removes one iron atom from the [4Fe-4S] cluster, being an active center of aconitase. In addition, the incurred changes in cell redox equilibrium may affect lipid metabolism. The aim of the study was to evaluate a concomitant effect of both drugs on hepatic aconitase activity and triglycerides level. In our study, doxorubicin (1.8 mg/kg b.w.) was administered intraperitoneal (i.p.) six times, once a week, within male Wistar rats, to achieve a cumulative dose of 10.8 mg/kg b.w. Two hours before every doxorubicin administration, tirapazamine in the dose of either 5 or 10 mg/kg b.w. was also i.p. injected. A week after withdrawing drug administration, the liver was taken for biochemical analysis. Therein, an increase in aconitase activity and a decrease in triglycerides level was seen in all groups exposed to doxorubicin. Our work demonstrated that tirapazamine administration had no influence on both tested parameters, but its higher dose rate normalized aconitase activity affected by doxorubicin.

14

The effect of thyroxin on hepatic redox equilibrium and lipid metabolism in rats treated with doxorubicin

88%

Czuba B., Fituch M., Mandziuk S., Jodlowska-Jedrych B., Matysiak W., Halasa J., Burdan F., Korga A., Iwan M., Luszczewska-Sierakowska I., Dudka J.

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vol. 27

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issue 4

220-223

EN

The main side effects of the administration of doxorubicin, a widely used anticancer drug, is the generation of a reactive oxygen species (ROS) in normal cells. As a result, redox disorders and secondary oxidative stress are developed. Doxorubicin ROS generation is attributed to enzymes that are produced abundantly in hepatocytes. Oxidative stress has been a well-known risk factor of doxorubicin-related toxicity. However, in addition, according to the data collected in the last decade, changes in thyroxin status can propagate ROS generation, and, thus, initiate the doxorubicin hepatic effect. Moreover, both compounds have an impact on the cell metabolism. The aim of the study was to verify the thesis that thyroxin can modulate the effect of doxorubicin with regard to redox status and lipid metabolism disorders. In our work, we determined the ratio of NADP+/ NADPH and NAD+/NADH in liver homogenates, blood ketone bodies and triglycerides in the liver and blood in rats treated with doxorubicin and thyroxin. Our results indicate that thyroxin has an insignificant effect on NAD+/NADH, NADP+/NADPH ratios and on hepatic and blood triglycerides. Moreover, thyroxin administration normalized the level of blood ketone bodies that was disturbed by doxorubicin.

15

Influence of acetaminophen and trichloroethylene on liver cytochrome P450-dependent monooxygenase system.

88%

Plewka A., Zielińska-Psuja B., Kowalówka-Zawieja J., Nowaczyk-Dura G., Plewka D., Wiaderkiewicz A., Kamiński M., Orłowski J.

Acta Biochimica Polonica

|

2000

|

vol. 47

|

issue 4

1129-1136

EN

The aim of the study was to evaluate the effect of acetaminophen (APAP) and/or trichloroethylene (TRI) on the liver cytochrome P450-dependent monooxygenase system, CYP2E1 and CYP1A2 (two important P450 isoforms), and liver glutathione (GSH) content in rats. Rats were given three different doses of APAP (250, 500 and 1000 mg/kg b...) and then the above-mentioned parameters were measured for 48 h. The lowest APAP dose produced small changes in the cytochrome P450 content of liver. At 500 mg/kg APAP increased the cytochrome P450 content to 230% of the control. The inductive effect was seen at 1000 mg/kg dose but at 24 h and later. NADPH-cytochrome P450 reductase activity was the highest after the lowest dose of APAP, while after the highest dose it was equal to the control value. TRI increased both the cytochrome P450 content and the NADPH-cytochrome P450 reductase activity. When TRI was combined with APAP, both these parameters increased in the first hours of observation, but they returned to the control values at 24 h. When APAP was given at 250 mg/kg, GSH levels decreased to 55% of the control at 8 h and returned to the control values at 24 h. The higher doses of APAP decreased GSH levels more than the lowest dose, but after 24 h GSH levels did not differ from those of the control. When TRI was given at 250 mg/kg, the GSH levels decreased to 68% of the control at 2 h and then they increased gradually and tended to exceed the control values at 48 h. The effect of TRI combined with APAP on the level of GSH was virtually the same as that of APAP alone given at 500 mg/kg.

16

Tissue variation of mitochondrial oxidative phosphorylation efficiency in cold-acclimated ducklings

88%

Salin K., Teulier L., Rey B., Rouanet J.-L., Voituron Y., Duchamp C., Roussel D.

Acta Biochimica Polonica

|

2010

|

vol. 57

|

issue 4

409-412

EN

We investigated the oxidative phosphorylation efficiency of liver and gastrocnemius muscle mitochondria in thermoneutral and cold-acclimated ducklings. The yield of oxidative phosphorylation was lower in muscle than in liver mitochondria, a difference that was associated with a higher proton conductance in muscle mitochondria. Cold exposure did not affect oxidative phosphorylation efficiency or basal proton leak in mitochondria. We conclude that the basal proton conductance of mitochondria may regulate mitochondrial oxidative phosphorylation efficiency, but is not an important contributor to thermogenic processes in cold-acclimated ducklings.

17

Effect of Ageratina adenophora on hepatic and pulmonic pathological lesions in horses

75%

Gu X. L., Dai F. Y., Xiao X., Li G. Z., Zhang L. M., Qu W. J., Gu X. L., Dai F. Y., Xiao X., Li G. Z., Zhang L. M., Qu W. J.

Polish Journal of Veterinary Sciences

|

2021

|

vol. 24

|

issue 4

18

Influence of simvastatin on hepatocytes – histopathological and immunohistochemical study

75%

Mikiewicz M., Otrocka-Domagała I., Paździor-Czapula K., Mikiewicz M., Otrocka-Domagała I., Paździor-Czapula K.

Polish Journal of Veterinary Sciences

|

2019

|

issue 2

19

Effect of kebar grass extract ( Biophytum petersianum Klotzsch) on histopathological changes in liver of mice offspring from the parent exposed to carbofuran during lactation period

75%

Yulitasari N. A., Hidanah S., Widjiati, Hendrawan V. F., Luqman E. M., Yulitasari N. A., Hidanah S., Widjiati, Hendrawan V. F., Luqman E. M.

Polish Journal of Veterinary Sciences

|

2021

|

vol. 24

|

issue 4

20

Metabolic parameters in young turkeys fed diets with different inclusion levels of copper nanoparticles

75%

Kozłowski K., Jankowski J., Otowski K., Zduńczyk Z., Ognik K.

Polish Journal of Veterinary Sciences

|

2018

|

vol. 21

|

issue 2

Refine search results

Molecular cloning and expression analysis of a new gene for short-chain dehydrogenase/reductase 9

Synchronous occurrence of multiple focal nodular hyperplasia and huge hepatic Perivascular epithelioid cells tumor (PEComa) in young woman after oral contraceptive use – is there a common pathogenesis?

An unusual case of a tuberculous granuloma of the liver presenting thirteen years after intravesical BCG – therapy for bladder cancer

Investigation of the hepatoprotective properties of “Phytovenol” capsules on the model of medicamentous hepatitis in rats

Cartilaginous compression of the liver – clinical and ultrasonographic aspects

Detailed EFSUM recommendations on the scope of ultrasound assessment in patients with portal hypertension considering the diagnostic reference level

Methylation demand: a key determinant of homocysteine metabolism.

The influence of single application of paracetamol and/or N-acetylcysteine on rats in subchronic exposition to trichloroethylene vapours. II. Effect on hepatic glutathione level

The influence of single application of paracetamol and/or N-acetylcysteine on rats in subchronic exposition to trichloroethylene vapours. III. Effect on some isoforms of cytochrome P450 in liver

Silver (Ag) in tissues and eggshells, biochemical parameters and oxidative stress in chickens

The influence of single application of paracetamol and/or N-acetylcysteine on rats subchronic exposed to trichloroethylene vapours. I. Effect on hepatic moonooxygenase system dependent of cytochrome P450

The effects of galactosamine on UTP levels in the livers of young, adult and old rats.

The effect of one-electron reduced drugs on hepatic aconitase activity and triglycerides level

The effect of thyroxin on hepatic redox equilibrium and lipid metabolism in rats treated with doxorubicin

Influence of acetaminophen and trichloroethylene on liver cytochrome P450-dependent monooxygenase system.

Tissue variation of mitochondrial oxidative phosphorylation efficiency in cold-acclimated ducklings

Effect of Ageratina adenophora on hepatic and pulmonic pathological lesions in horses

Influence of simvastatin on hepatocytes – histopathological and immunohistochemical study

Effect of kebar grass extract ( Biophytum petersianum Klotzsch) on histopathological changes in liver of mice offspring from the parent exposed to carbofuran during lactation period

Metabolic parameters in young turkeys fed diets with different inclusion levels of copper nanoparticles