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Vitamin D in the skin physiology and pathology

100%

Piotrowska A., Wierzbicka J., Żmijewski M.

Acta Biochimica Polonica

2016

vol. 63

issue 1

17-29

Vitamin D plays important, pleiotropic role in the maintenance of global homeostasis. Its influence goes far beyond the regulation of calcium and phosphorus balance, as diverse activities of vitamin D and its natural metabolites assure proper functioning of major human organs, including skin. Recently, we reviewed the current understanding of vitamin D impact on human health from historical perspective (Wierzbicka et al. (2014) The renaissance of vitamin D. Acta Biochim Pol 61: 679-686). This article focuses on its functions in the skin. The skin and its appendages, creates a platform connecting and protecting internal organs against, usually harmful, external environment. It uppermost layer - epidermis in order to maintain a protective barrier undergoes a constant exchange of cornified keratinocytes layer. Its disturbance leads to development of serious skin disorders including psoriasis, vitiligo, atopic dermatitis and skin cancer. All of those dermatopathologies have a huge impact on modern societies, affecting not only the physical, but also mental state of patients as well as their social status. Furthermore, multiple human systemic diseases (autoimmune, blood and digestive diseases) have skin manifestation, thus "condition of the skin" often reflects the condition and pathological changes within the internal organs. In humans, the skin is the natural source of vitamin D, which is produced locally from 7-dehydrocholesterol in photoreaction induced by ultraviolet B (UVB) radiation from the sun. It is also well established, that the process of proliferation and differentiation of keratinocytes is tightly regulated by calcium and the active form of vitamin D (1,25(OH)2D3). Thus, the skin physiology is inseparably connected with vitamin D production and activity. Unfortunately, UVB, which is required for vitamin D production, is also known as the main cause of a skin cancer, including melanoma. Here, we are going to review benefits of vitamin D and its analogues in the maintenance of epidermal barrier and its potential use in the treatment of common skin diseases.

Own experience from the use of a substitute of an allogeneic acellular dermal matrix revitalized with in vitro cultured skin cells in clinical practice

100%

Łabuś W., Kawecki M., Glik J., Maj M., Kitala D., Misiuga M., Klama-Baryła A., Kraut M., Nowak M.

Polish Journal of Surgery

2015

vol. 87(10)

513-521

As a result of the removal of cells from human allogeneic dermis, a collagen scaffold is obtained, which can be populated de novo with autologous/allogeneic skin cells and transplanted onto the area of skin loss. The optimal method for production of acellular dermal matrices (ADM) has been selected. Three female patients (a mean age of 54 years) were subjected to the transplantation of either autologous or allogeneic keratinocytes and fibroblasts into the holes of acellular dermal matrix (ADM) mesh graft. The method for burn wound treatment based on the use of a viable dermal-epidermal skin substitute (based on ADM and in vitro cultured fibroblasts and keratinocytes) may be the optimal method of burn treatment.

Proliferation and Ability for Epidermal Autoregeneration in Patients with Chronic Lower Leg Venous Ulcerations

86%

Waligóra J., Noszczyk B.

Polish Journal of Surgery

2007

vol. 79

issue 2

113-119

The protein p63 plays a significant role in the development of animal epithelium. p63 is a regulator of differentiation, senescence and adhesion programs in numerous mature epithelial tissues. In patients with a healthy epidermis, p63 maintains cell progenitor potential - the ability for cellular division to occur using the delayed differentiation program. It is also responsible for the protecting the epithelial phenotype from depletion in migrating cells, thus resulting in invasion and infiltration after altering its endogenous expression.The aim of the study was to compare the number of cells with p63 protein expression and the presence of Ki67 proliferation marker in the epidermis in patients with chronic venous ulcerations versus those with properly healing wounds.Material and methods. Study materials were comprised of biopsy samples collected from healthy volunteers and patients treated for venous ulcerations. The specimens were subjected to immunohistochemical staining using available monoclonal antibodies and were analyzed with an imaging analysis program which evaluated the expression indices of both proteins in areas of intensified cellular division, i.e. wound edges.Results. The number of cells displaying protein expression in patients with chronic venous ulcerations was significantly lower in comparison to the values observed in healthy volunteers. This was determined during the intermediary phase of wound healing, the most pronounced phase of cellular response to injury.Conclusions. Decreased epidermal p63 expression in patients with venous ulcerations suggests insufficient protein production for the maintenance of autoregeneration and long-lasting division; both are required for the supplementation of migrating cells. The above-mentioned phenomenon suggests that there may be a role for p63 in regulation of the healing process and pathophysiology of chronic venous leg ulcerations.

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2 Polish Journal of Surgery

1 Acta Biochimica Polonica

1 2016

1 2015

1 2007

1 Glik J.

1 Kawecki M.

1 Kitala D.

1 Klama-Baryła A.

1 Kraut M.

1 Maj M.

1 Misiuga M.

1 Noszczyk B.

1 Nowak M.

1 Piotrowska A.

1 Waligóra J.

1 Wierzbicka J.

1 Łabuś W.

1 Żmijewski M.

Search results

Vitamin D in the skin physiology and pathology

Own experience from the use of a substitute of an allogeneic acellular dermal matrix revitalized with in vitro cultured skin cells in clinical practice

Proliferation and Ability for Epidermal Autoregeneration in Patients with Chronic Lower Leg Venous Ulcerations