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1

Genetic variability of the μ-opioid receptor influences intrathecal fentanyl analgesia requirements in laboring women

100%
Landau R. L., Kern C., Columb M. O., Smiley R. M., Blouin J.-L.
Ból
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2009
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vol. 10
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issue 1
EN
Labor initiates one of the most intensely painful episodes in a woman’s life. Opioids are used to provide analgesia with substantial interindividual variability in efficacy. μ-opioid receptor (μ OR, OPRM1) genetic variants may explain differences in response to opioid analgesia. We hypothesized that OPRM1 304A/G polymorphism influences the median effective dose (ED50) of intrathecal fentanyl via combined spinal-epidural for labor analgesia. Nulliparous women were prospectively recruited around 35 weeks gestation (n=224), and genotyped for 304A/G polymorphism. Those requesting neuraxial labor analgesia were enrolled in one of the two double-blinded trials: up-down sequential allocation (SA, n=50) and a separate confirmatory random-dose allocation trial (RA, n=97). Effective analgesia from intrathecal fentanyl was defined by P60 min analgesia with verbal rating score 61 (scale 0–10) and was compared between μ OR 304A homozygotes (Group A) and women carrying at least one 304G allele (Group G). OPRM1 304G allele frequency f(-) was 0.18. Using SA, intrathecal fentanyl ED50 was 26.8 lg (95% CI 22.7–30.9) in Group A and 17.7 μg (95% CI 13.4–21.9) in Group G (p<0.001; 304A homozygosity increased the ED50 1.5-fold). RA confirmed that 304A homozygosity significantly increases intrathecal fentanyl ED50 (27.4 μg in Group A and 12.8 μg in Group G [p<0.002; 2.1-fold]). We demonstrate for the first time that the μ OR 304G variant significantly reduces intrathecal fentanyl ED50 for labor analgesia, suggesting women with the G variant may be more responsive to opioids and require less analgesic drugs. These findings for intrathecal fentanyl pharmacogenetics may have implications for patients receiving opioids in other settings.
PL
Poród stanowi jedno z najintensywniejszych doznań bólowych w życiu kobiety. Do analgezji używa się opioidów; cechują się one jednak istotną zmiennością w zakresie skuteczności u poszczególnych osób. Odmiany genetyczne receptora opioidowego μ (ROμ, OPRM1) mogą tłumaczyć różnicę w odpowiedzi na analgezję opioidową. Zaproponowaliśmy hipotezę, według której polimorfizm 304A/G genu OPRM1 wpływa na medianę skutecznej dawki (ED50) fentanylu podawanego dokanałowo w łączonej analgezji podpajęczynówkowo-zewnątrzoponowej w przebiegu porodu. Do badania w sposób prospektywny włączono pierworódki będące w około 35 tygodniu ciąży (n=224); wykonano u nich genotypowanie polimorfizmu 304A/G. Pacjentki zgłaszające potrzebę blokady centralnej w trakcie porodu włączano do jednego z dwóch badań klinicznych prowadzonych metodą podwójne ślepej próby: w pierwszym z nich dawki przypisywano sekwencyjnie metodą „w górę lub w dół” (grupa przypisania sekwencyjnego, PS; n=50); w odrębnym potwierdzającym badaniu dokonywano losowego przypisania do dawki leku (grupa przypisania losowego, PL; n=97). Skuteczną analgezję przy użyciu fentanylu podawanego dokanałowo definiowano jako analgezję ≥60 min. z oceną słowną natężenia bólu ≤1 (skala od 0 do 10) i porównywano między pacjentkami, będącymi homozygotami pod względem ROμ 304A (Grupa A) i kobietami, które były nosicielkami co najmniej jednego allelu G (Grupa G). Częstość występowania allelu 304G OPRM1 ƒ(-) wyniosła 0,18. W badaniu z PS, ED50 dla fentanylu podawanego dokanałowo wyniosła 26,8 μg (95% CI: 22,7–30,9) w grupie A i 17,7 μg (95% CI: 13,4–21,9) w grupie G (p<0,001; homozygotyczność względem 304A zwiększała ED50 o 1,5 raza). Badanie z PL potwierdziło, że homozygotyczność względem allelu 304A istotnie zwiększa ED50 fentanylu podawanego dokanałowo (27,4 μg w Grupie A i 12,8 μg w Grupie G [p<0,002; 2,1-krotnie]). Wykazaliśmy po raz pierwszy, że wariant 304G ROμ znamiennie zmniejsza ED50 fentanylu podawanego dokanałowo w celu uzyskania analgezji podczas porodu, co wskazuje, że kobiety z wariantem G mogą lepiej reagować na opioidy i wymagać mniejszych dawek leków przeciwbólowych. Przedstawione wyniki dotyczące farmakogenetyki fentanylu podawanego dokanałowo mogą być istotne dla pacjentów, którzy otrzymują opioidy z innych powodów.
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Structure and biosynthesis of human salivary mucins.

100%
Zalewska A., Zwierz K., Żółkowski K., Gindzieński A.
Acta Biochimica Polonica
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2000
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vol. 47
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issue 4
1067-1079
EN
Human salivary glands secrete two types of mucins: oligomeric mucin (MG1) with molecular mass above 1 MDa and monomeric mucin (MG2) with molecular mass of 200-250 kDa. Monomers of MG1 and MG2 contain havily O-glycosylated tandem repeats located at the central domain of the molecules. MG1 monomers are linked by disulfide bonds located at sparsely glycosylated N- and C-end. MG1 are synthesized by mucous cells and MG2 by the serous cells of human salivary glands.
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Kinematic Analysis of Seating Maneuver: Digitalization of Movement in Daily Living

100%
Takei M., Shimizu H., Hoshiyama M.
Human Movement
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2010
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vol. 11
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issue 2
114-118
EN
Purpose. The objective of the present study was to digitally express a common daily movement of sitting down (seating maneuver), and to show an analytical example of normative indices of such a daily movement. Basic procedures. Sequential traces of moments and the center of pressure (COP) during the seating maneuver, approaching with steps to and sitting on a stool, were measured using two force plates, and we decided on normal ranges of parameters based on the vertical moment and lateral deviation of the COP. In addition to the normal data recorded from ten healthy subjects, a data set from a patient was plotted. Main findings. Normative indices to express the sequential movement were obtained. The patient showed abnormal values of the indices, which could be quantitative indicators to evaluate the normality and grade of abnormality. Conclusions. We introduced a method for the quantitative screening of a daily movement using force plates. The results showed normative values, and the method could be used to reveal abnormalities in a daily movement in a patient with mild movement disability.
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The in vitro modulatory effect of TNFα on the mRNA expression and protein levels of zinc finger protein ZNF334 in CD4+ lymphocytes of healthy people

100%
Henc I., Soroczyńska-Cybula M., Bryl E., Witkowski J.
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issue 1
113-117
EN
We have shown before that the expression of ZNF334 gene, coding for a newly described zinc finger protein of as yet unknown function, is extremely reduced in CD4+ lymphocytes of rheumatoid arthritis (RA) patients regardless of their age, and thus can be considered a new molecular marker of the disease. Based on the promoter sequence of the gene we speculated that it might be regulated by TNFα. Here we have tested that hypothesis, studying the in vitro influence of TNFα on the ZNF334 gene expression and protein levels in resting and stimulated CD4+ cells of healthy volunteers. We have confirmed that treatment with TNFα modifies the levels of ZNF334 expression in the CD4+ cells ex vivo; however, the effect varied for different individuals and reduction of expression was seen only for those cell samples that initially exhibited high transcriptional activity of the gene, while for those exhibiting initially very low expression, some increase in the transcriptional activity was observed. Incubation with TNFα significantly reduced the amounts of two isoforms of ZNF334 protein (initially high in all subjects) in parallel to the reduced transcription. Finally, the expression of ZNF334 in CD4+ lymphocytes isolated after various periods of anti-CD3 stimulation generally increased with longer culture times, and the effect of TNFα treatment was negligible. Concluding, our results obtained in vitro for helper lymphocytes of healthy individuals seem to mimic the regulatory effect of TNFα on the expression of ZNF334 in the cells of RA patients.
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Heteroplasmy analysis in the Polish patients with 11778A mutation responsible for Leber hereditary optic neuropathy.

100%
Mroczek-Tońska K., Ratajska D., Guillot C., Sąsiek M., Ambroziak A., Lubos L., Bartnik E.
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vol. 49
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issue 1
257-262
EN
We have analysed the heteroplasmy level in 11 individuals from 3 families harbouring the mitochondrial 11778A mutation responsible for Leber hereditary optic neuropathy using last cycle hot PCR. The mutation level exceeded 90% both in affected and in unaffected individuals. We also checked whether any of the families belonged to the J haplogroup of mitochondrial DNA and obtained a negative result.
6
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Cytogenetic effects in human as the result of the Chernobyl accident

100%
Pilinskaya M. A.
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vol. 1
18-22
EN
The Chernobyl accident significantly complicated the ecological situation in Ukraine. The existing environmental pollution, which was already rooted in the background, ensures for the most part that the Ukrainian population comes into contact with one of the most powerful universal mutagens - ionizing radiation. This can cause genetic damage in all living beings, including human. As such, it is essential that the Chernobyl accident victims be medically monitored using cytogenetic monitoring – the study of the frequency and spectrum of chromosome aberrations in human peripheral blood lymphocytes with the help of classical (conventional cytogenetics, G-banding analysis) and new (FISHWCP) research methods.
7
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Chemical composition of human and canine fascia lata

88%
Maksymowicz K., Marycz K., Szotek S., Kaliński K., Serwa E., Łukomski R., Czogała J.
Acta Biochimica Polonica
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2012
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vol. 59
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issue 4
531-535
EN
The fascial system is an integral part of the musculoskeletal system. It is a three-dimensional network of connective tissue spreading ubiquitously throughout the body, surrounding muscles, bones, internal organs, nerves, vessels, and other structures. The basic biophysical properties of the fascial system are determined by its structure and chemical composition. This study aimed to determine the elemental composition of pathologically unchanged fascia lata of the thigh, collected during autopsies on humans and dogs. The wide spectrum of elements analysed included both macro and micro elements. The analyses were conducted using scanning electron microscopy with X-ray microanalysis (SEM-EDS). Concentrations of the following macro and micro elements were dermined: C, N, O, Na, Mg, Al, Si, P, S, Cl, K, Ca, Ti, Fe Co, Ni, Cu, and Zn. The obtained results showed significant differences between human and canine fascia lata regarding the content of most of the examined elements (p < 0.05), except for N. These data may in future provide a starting point for the establishment of reference values for the content of various elements in normal fascial tissue and may also serve to verify the usefulness of experimental animal material as a substitute for human tissue.
8
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Tocopherol esters inhibit human glutathione S-transferase omega

88%
Sampayo-Reyes A., Zakharyan R. A.
Acta Biochimica Polonica
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2006
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vol. 53
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issue 3
547-552
EN
Human glutathione S-transferase omega 1-1 (hGSTO1-1) is a newly identified member of the glutathione S-transferase (GST) family of genes, which also contains alpha, mu, pi, sigma, theta, and zeta members. hGSTO1-1 catalyzes the reduction of arsenate, monomethylarsenate (MMA(V)), and dimethylarsenate (DMA(V)) and exhibits thioltransferase and dehydroascorbate reductase activities. Recent evidence has show that cytokine release inhibitory drugs, which specifically inhibit interleukin-1b (IL-1b), directly target hGSTO1-1. We found that (+)-α-tocopherol phosphate and (+)-α-tocopherol succinate inhibit hGSTO1-1 in a concentration-dependent manner with IC50 values of 2 µM and 4 µM, respectively. A Lineweaver-Burk plot demonstrated the uncompetitive nature of this inhibition. The molecular mechanism behind the inhibition of hGSTO1-1 by α-tocopherol esters (vitamin E) is important for understanding neurodegenerative diseases, which are also influenced by vitamin E.
9
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The Primary Noise Control in the Work Environment by Increasing the Quality of Bearings and Effective Mounting of Machines

75%
Žiaran S., Šooš Ľ., Chlebo O., Žiaran S., Šooš Ľ., Chlebo O.
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2020
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vol. 45
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issue 2
10
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High levels of bulky DNA adducts in human sperm correlate with impaired fertility.

75%
Horak S., Polańska J., Widłak P.
Acta Biochimica Polonica
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2003
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vol. 50
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issue 1
197-203
EN
Progressive decline in fertility and sperm quality has been reported over the last few decades, especially in industrialized nations. It has been proposed that exposure to factors that induce damage in DNA of spermatogenic cells may significantly contribute to impaired fertility. Here, the 32P-postlabelling method was used to analyze the levels of bulky DNA adducts in sperm cells in a group of 179 volunteers, either healthy subjects or patients with an impaired fertility. The levels of DNA adducts were 1.35-fold higher in the infertile group as compared to healthy individuals (P = 0.012). Similarly, a significant negative correlation between the levels of DNA adducts and measures of semen quality (sperm concentration and motility) has been observed (P Ł 0.001). In addition, the levels of bulky DNA adducts in sperm cells positively correlates with amounts of leukocytes in semen, which were significantly higher in semen of infertile subjects.
11
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Wpływ masażu na psychofizyczny aspekt jakości życia pacjenta

63%
Dymacz M., Materkowski M.
Polish Journal of Sports Medicine
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2020
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vol. 36(1)
31-47
EN
Background: The aim of the study is to investigate whether classical massage affects the psychophysical state of human by performing full body massage on different patient groups. The historical outline of classical massage, the massage methodology (including information about the room and the preparation of the masseur, the preparation and positioning of the patient, the general rules of the procedure and the basic claws of classical massage), the indications and contraindications for the massage, the effect on the human body and the place of the form Massage in the SPA. Materials and methods: The study included totally 30 patients of different ages. The tested women were twenty-two and eight men. All patients underwent a full body classical massage with slipping (on the olive). The massage was performed for 50 minutes. Based on the author's survey examined feelings about the health status, life satisfaction, stress level, well-being level, physical condition before and after the classical massage. Results: The results show the beneficial effect of classical massage on the human body and psyche. Already after one treatment, improvements in health, well-being, stress, physical well-being and life satisfaction were observed. These differences were statistically significant. Conclusions: Classical massage has a positive effect on the body and human psyche.
PL
Wstęp: Celem pracy jest szczegółowe zbadanie czy masaż klasyczny wpływa na stan psychofizyczny człowieka, poprzez wykonanie masażu całościowego na różnych grupach pacjentów. Przedstawiono zarys historyczny masażu klasycznego, metodykę wykonania masażu (zawierającą między innymi informacje o ogólnych zasadach wykonania zabiegu oraz podstawowych chwytach masażu), wskazania i przeciwwskazania do stosowania masażu, działanie masażu na organizm człowieka oraz miejsce formy masażu w SPA. Materiał i metody: W badaniu wzięło udział 30 pacjentów w różnym wieku. Liczba kobiet to 22, natomiast mężczyzn 8. U wszystkich osób badanych wykonano masaż klasyczny całego ciała z poślizgiem (na oliwce). Masaż trwał 50 minut. Poprzez stworzenie autorskiej ankiety zbadano odczucia badanych pacjentów dotyczące stanu zdrowia, zadowolenia z życia, poziomu stresu, poziomu samopoczucia, stanu fizycznego przed i po wykonanym zabiegu. Wyniki: Otrzymane wyniki wskazują na korzystne efekty wpływu masażu klasycznego na organizm i psychikę człowieka. Już po jednym zabiegu zaobserwowano subiektywną poprawę stanu zdrowia, samopoczucia, spadek stresu, poprawę stanu fizycznego i zadowolenia z życia. Różnice te były istotne statystycznie. Wnioski: Masaż klasyczny wpływa pozytywnie na organizm i psychikę pacjenta.
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