Search results

1

Differentiation by random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) of Candida albicans isolated from upper respiratory tract in patients with non-small cell lung cancer

100%

Biernasiuk A., Korona-Głowniak I., Grzegorczyk A., Malm A.

Acta Biochimica Polonica

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2014

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vol. 61

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issue 4

727-729

EN

Cancer patients are predisposed to fungal infections caused by Candida albicans, especially to oral or respiratory tract candidiasis. The aim of this study was to estimate genetic diversity by RAPD-PCR (random amplified polymorphic DNA-polymerase chain reaction) of C. albicans isolated from upper respiratory tract of 100 patients with non-small cell lung cancer. Among 52 strains, 34 genotypes were defined. 10 clusters comprising 28 (53.85%) isolates with similarity coefficient ≥ 80% were formed. The remaining 24 (46.15%) isolates represented individual genotypes. The RAPD-PCR technique revealed genomic variability within C. albicans isolated from upper respiratory tract of the cancer patients.

2

Evidence for RNA recombination between distinct isolates of Pepino mosaic virus

100%

Hasiów-Jaroszewska B., Kuzniar A., Peters S. A., Leunissen J. A. M., Pospieszny H.

Acta Biochimica Polonica

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2010

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vol. 57

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issue 3

385-388

EN

Genetic recombination plays an important role in the evolution of virus genomes. In this study we analyzed publicly available genomic sequences of Pepino mosaic virus (PepMV) for recombination events using several bioinformatics tools. The genome-wide analyses not only confirm the presence of previously found recombination events in PepMV but also provide the first evidence for double recombinant origin of the US2 isolate.

3

Full length genome sequence of Polish isolate of Beet soil-borne virus confirms low level of genetic diversity

100%

Borodynko N., Hasiów-Jaroszewska B., Rymelska N., Pospieszny H.

Acta Biochimica Polonica

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2009

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vol. 56

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issue 4

729-731

EN

The complete nucleotide sequence of a Polish isolate of Beet soil-borne virus was determined for the first time. The genome organization was identical with those previously established for isolates from Germany and China. A comparison of the Polish isolate with others deposited in GenBank reveled high level of nucleotide identity, about 98-100%, throughout the genome analyzed. The ratio between non-synonymous and synonymous substitutions was rather low suggesting a negative selective pressure. The non-synonymous mutations were particulary frequent in triple gene block.

4

Pochodzenie i zróżnicowanie genetyczne ludzkiego wirusa niedoboru odporności typu 1

88%

Ziach-Terlecka M., Wąsik T.

Annales Academiae Medicae Silesiensis

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2013

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vol. 67

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issue 6

400-409

EN

Multiple transmission of simian immunodeficiency virus (SIV) into the human population started the pandemic of human immunodeficiency virus (HIV-1). The extraordinary variability of human immunodeficiency virus HIV-1 resulting from the high rate of genome mutations and the recombination between different strains resulted in the emergence of a genetically diverse viral strains. Genetic differences between HIV-1 isolates are now the basis for the classification of the virus. Tracking the spread of the HIV-1 genetic variants allows for the monitoring of the development of the epidemic, and provides important information about the time and possible routes of virus introduction into different countries, geographical regions or populations with different transmission routes of infection. On the other hand, the vast viral genetic diversity complicates the diagnosis of infection, can affect the susceptibility of HIV-1 to drugs and is a major obstacle in research aimed at creating an effective vaccine. In this paper the current knowledge on the origin of virus, the genetic diversity of HIV-1, as well as its impact on the course of infection and development of the epidemic is presented.

PL

Pandemia ludzkiego wirusa niedoboru odporności (human immunodeficiency virus – HIV) została zapoczątkowana przez wielokrotną transmisję małpiego wirusa upośledzenia odporności (simian immunodeficiency virus – SIV) do populacji ludzkiej. Niezwykła zmienność HIV-1 wynikająca z dużej częstości mutacji pojawiających się w genomie oraz możliwości rekombinacji między materiałem genetycznym różnych szczepów wirusa zaowocowała wykształceniem zróżnicowanych pod względem genetycznym wariantów. Genetyczne różnice między izolatami HIV-1 stanowią obecnie podstawę klasyfikacji wirusa, a śledzenie rozprzestrzeniania poszczególnych wariantów pozwala na monitorowanie rozwoju epidemii i dostarcza ważnych informacji na temat czasu i sposobu transmisji wirusa do różnych krajów, regionów geograficznych lub populacji. Z drugiej strony olbrzymia różnorodność form genetycznych wirusa utrudnia diagnostykę infekcji, może wpływać na wrażliwość HIV-1 na leki i stanowi poważną przeszkodę w badaniach zmierzających do stworzenia skutecznej szczepionki. W niniejszej pracy przedstawiono aktualny stan wiedzy na temat pochodzenia wirusa, genetycznego zróżnicowania HIV-1 i wpływu tego czynnika na przebieg zakażenia i rozwój epidemii.

5

Low genetic differentiation between two morphotypes of the gastropod Nacella concinna from Admiralty Bay, Antarctica

88%

Chwedorzewska K. J., Korczak M., Bednarek P. T., Markowska-Potocka M., Chwedorzewska K. J., Korczak M., Bednarek P. T., Markowska-Potocka M.

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issue 2

6

Mitochondrial DNA variability in Gyimesi Racka and Turcana sheep breeds

88%

Kusza S., Zakar E., Budai C., Cziszter L.-T., Padeanu I., Gavojdian D.

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issue 2

273-280

EN

The current knowledge and documentation on the origins and relationship between Gyimesi Racka reared in Hungary and the Romanian Turcana is rather controversial. Lack of information and scientific reliable proofs for the divergent theories found in the two countries motivated us to implement a trial using molecular methods to assess the genetic distance and diversity in the two breeds. Hair follicles were collected from Gyimesi Racka (2 phenotypes) and from Turcana (6 ecotypes). The 599 bp segment of the D-loop region of the mitochondrial DNA was sequenced. Altogether, 42 haplotypes were identified, while 23 were found in both populations. Populations were highly diverse according to the haplotype and nucleotide diversity indices. AMOVA analysis showed that most of the variation was observed within populations (98%), indicating a weak genetic structure between the two breeds. Animals were grouped into seven groups based on their phenotype; however genetic distances among them were also low. Tajima's D, Fu's Fs, goodness-of-fit statistics, mismatch distribution and network analysis suggested recent demographic expansion. Current comprehensive mtDNA study indicates that there is very low level of genetic differentiation between the Gyimesi Racka and Turcana populations therefore they are de facto one trans-boundary breed.

7

Genetic variability of Colobanthus quitensis from King George Island (Antarctica)

88%

Giełwanowska I., Androsiuk P., Chwedorzewska K., Szandar K.

Polish Polar Research

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2015

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issue 3

8

Genetic variability and virulence of some Iranian Rhizoctonia solani isolates associated with stem canker and black scurf of potato (Solanum tuberosum L.)

75%

Esfahani M. N., Esfahani M. N.

Journal of Plant Protection Research

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2020

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vol. 60

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issue 1

9

GENETIC DIVERSITY OF SALIX PURPUREA L. GENOTYPES AND INTERSPECIFIC HYBRIDS

75%

Sulima P., Przyborowski J. A.

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vol. 55

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issue 2

10

Morphological, virulence and genetic variability of Ulocladium atrum causing potato leaf blight disease in Iran

75%

Esfahani M. N.

Journal of Plant Protection Research

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2019

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vol. 59

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issue 1

11

Temporal isolation between two strongly differentiated stocks of the Greenland halibut ( Reinhardtius hippoglossoides Walbaum, 1792) from the Western Barents Sea

75%

Wojtasik B., Kijewska A., Mioduchowska M., Mikuła B., Sell J., Wojtasik B., Kijewska A., Mioduchowska M., Mikuła B., Sell J.

Polish Polar Research

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2021

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vol. 42

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issue 2

12

Genetic insights into ecological succession from oak- (Quercus robur L.) to beech-(Fagus sylvatica L.) dominated forest stands

75%

Sandurska E., Ulaszewski B., Burczyk J.

Acta Biologica Cracoviensia s. Botanica

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2017

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vol. 59

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issue 1

13

Genetic diversity study of Fusarium culmorum: causal agent of wheat crown rot in Iraq

75%

Matny O., Shamsallah S., Al Fahad M., Haas M., Matny O., Shamsallah S., Al Fahad M., Haas M.

Journal of Plant Protection Research

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2019

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vol. 59

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issue 2

14

A Multi-gene Analysis Reveals Multiple Highly Divergent Lineages of The Isopod Chelator Insignis (Hansen, 1916) South of Iceland

75%

Brix S., Svavarsson J., Leese F.

Polish Polar Research

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2014

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issue 2

15

Genetic diversity and differentiation of coexisting populations of Quercus robur L.and Q. petraea (Matt.) Liebl.

63%

Sandurska E., Ulaszewski B., Burczyk J.

Acta Biologica Cracoviensia s. Botanica

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2019

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vol. 61

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issue 1

16

Metody oparte o amplifikację DNA techniką PCR wykorzystywane w ocenie bioróżnorodności mikroorganizmów glebowych

51%

Łyszcz M., Gałązka A.

Kosmos

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2017

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vol. 66

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issue 2

193-206

PL

Mikroorganizmy glebowe, pod względem cech genomowych i fenotypowych, stanowią wysoce zróżnicowaną grupę organizmów żywych. Z powodu tak dużej różnorodności ważne jest dobranie odpowiednich metod, dających największy stopień różnicowania mikroorganizmów. Narzędziami umożliwiającym analizę zmienności genetycznej mikroorganizmów są techniki genetyczne, a wśród nich jedną z najważniejszych jest łańcuchowa reakcja polimerazy, czyli PCR (Polymerase Chain Reaction), technika opracowana w latach 1980. Niniejsza praca stanowi przegląd podstawowych zagadnień dotyczących badania zmienności genetycznej mikroorganizmów glebowych w oparciu o markery molekularne z wykorzystaniem technik bazujących na reakcji PCR tj. PCR-RFLP, TRFLP, ARDRA, RAPD.

EN

Soil microorganisms represent a highly diverse group of living organisms in terms of genomic and phenotypic characteristics. Due to such a large diversity, it is important to select appropriate identification methods which would secure its most complete determination. Genetic techniques are proper tools of choice for analyzing genetic variability of microorganism, the most important of which is the polymerase chain reaction (PCR), developed in the 1980s. This work presents an overview of the basic issues concerning studies on genetic variability of soil microorganisms with help of molecular markers and application of PCR techniques such as PCR-RFLP, TRFLP, ARDRA, RAPD.

Refine search results

Differentiation by random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) of Candida albicans isolated from upper respiratory tract in patients with non-small cell lung cancer

Evidence for RNA recombination between distinct isolates of Pepino mosaic virus

Full length genome sequence of Polish isolate of Beet soil-borne virus confirms low level of genetic diversity

Pochodzenie i zróżnicowanie genetyczne ludzkiego wirusa niedoboru odporności typu 1

Low genetic differentiation between two morphotypes of the gastropod Nacella concinna from Admiralty Bay, Antarctica

Mitochondrial DNA variability in Gyimesi Racka and Turcana sheep breeds

Genetic variability of Colobanthus quitensis from King George Island (Antarctica)

Genetic variability and virulence of some Iranian Rhizoctonia solani isolates associated with stem canker and black scurf of potato (Solanum tuberosum L.)

GENETIC DIVERSITY OF SALIX PURPUREA L. GENOTYPES AND INTERSPECIFIC HYBRIDS

Morphological, virulence and genetic variability of Ulocladium atrum causing potato leaf blight disease in Iran

Temporal isolation between two strongly differentiated stocks of the Greenland halibut ( Reinhardtius hippoglossoides Walbaum, 1792) from the Western Barents Sea

Genetic insights into ecological succession from oak- (Quercus robur L.) to beech-(Fagus sylvatica L.) dominated forest stands

Genetic diversity study of Fusarium culmorum: causal agent of wheat crown rot in Iraq

A Multi-gene Analysis Reveals Multiple Highly Divergent Lineages of The Isopod Chelator Insignis (Hansen, 1916) South of Iceland

Genetic diversity and differentiation of coexisting populations of Quercus robur L.and Q. petraea (Matt.) Liebl.

Metody oparte o amplifikację DNA techniką PCR wykorzystywane w ocenie bioróżnorodności mikroorganizmów glebowych