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1

ISOLATION, MOLECULAR CLONING AND CHARACTERISATION OF TWO GENES CODING CHITIN DEACETYLASE FROM MUCOR CIRCINELLOIDES IBT-83

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Kaczmarek M., Struszczyk-Świta K., Florczak T., Szczęsna-Antczak M., Antczak T.

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EN

Chitosan is a linear N-deacetylated derivative of chitin, soluble in acetic solutions. The deacetylation of chitin can be achieved enzymatically using chitin deacetylase (ChDa) (EC 3.5.1.41), which hydrolyses the N-acetamido groups of N-acetyl-D-glucosamine residues in chitin and chitosan. Complementary DNA (cDNA), which encodes ChDa, was isolated from M. rouxii s well as other fungi. Chitin deacetylase activity was detected in partially purified and concentrated crude extract of the protein from Mucor circinelloides IBT-83. Additionally, two open reading frames (ORF), putatively encoding ChDa, were identified and amplified from cDNA of this strain. Each ORF was molecularly cloned and sequenced. Amino acid sequences of ChDaI and ChDaII were predicted, using nucleotide sequences of these cDNA clones, and analysed by means of bioinformatics tools.

2

THE GSTP1 c.313A>G POLYMORPHISM MODULATES THE CARDIORESPIRATORY RESPONSE TO AEROBIC TRAINING

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Zarebska A., Jastrzebski Z., Kaczmarczyk M., Ficek K., Maciejewska-Karlowska A., Sawczuk M., Leońska-Duniec A., Krol P., Cieszczyk P., Zmijewski P., Eynon N.

Biology of Sport

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2014

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vol. 31

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issue 4

261-266

EN

TThe GSTP1 c.313A>G polymorphism is a candidate to explain some of the individual differences in cardiorespiratory fitness phenotypes’ responses to aerobic exercise training. We aim to explore the association between the GSTP1 c.313A>G polymorphism and the response to low-high impact aerobic exercise training. Sixty-six Polish Caucasian women were genotyped for the GSTP1 c.313A>G polymorphism; 62 of them completed 12-week aerobic (50-75% HR max ) exercise training and were measured for selected somatic features (body mass and BMI) and cardiorespiratory fitness indices – maximal oxygen uptake (VO 2max , maximum heart rate (HR max ), maximum ventilation (V E max ) and anaerobic threshold (AT) – before and after the training period. Two-factor analysis of variance revealed a main training effect for body mass reduction (p=0.007) and BMI reduction (p=0.013), improvements of absolute and relative VO 2max (both p<0.001), and increased V E max (p=0.005), but not for changes in fat-free mass (FFM) (p=0.162). However, a significant training x GSTP1 c.313A>G interaction was found only for FFM (p=0.042), absolute and relative VO 2max (p=0.029 and p=0.026), and V E max (p=0.005). As the result of training, significantly greater improvements in VO 2max , V E max and FFM were gained by the GG+GA group compared to the AA genotype group. The results support the hypothesis that heterogeneity in individual response to training stimuli is at least in part determined by genetics, and GSTP1 c.313A>G may be considered as one (of what appear to be many) target polymorphisms to influence these changes.

3

Association and polymorphism study of seven candidate genes with reproductive traits in three pig breeds in Hungary

88%

Hunyadi-Bagi Á., Balogh P., Nagy K., Kusza S.

Acta Biochimica Polonica

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2016

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vol. 63

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issue 2

359-364

EN

Seven genes (BF, EGF, ESR, FSHB, H2AFZ, LEP and PRLP) were studied as candidate gene influencing eleven reproduction traits (interval between litters (IBL), percent of litter (PL), number of litters (NL), number of piglets born dead (NBD), number of piglet born alive (NBA), total number born (TNB), mean of born alive (MBA), mean of born dead (MBD), mean of born total (MBT), mean of piglets at 21 days of age (M21D) and growth rate (GR) in three pig breeds (Hungarian Large White (HLW), Duroc and Pietrain) by PCR-RFLP. Based on the observed vs. expected genotypes frequencies populations across loci were in Hardy-Weinberg equilibrium (P>0.05). In case HLW breed ESR and FSHβ genes were in disequilibrium. Association study suggested that only EGF gene showed significant influence on the trait NBA and TNB. The AA genotype are preferable for sows, associated with higher NBA and TNB. The longest IBL, and the highest NL is associated with AB and AA genotype of EGF gene. IBL is significantly shorter in case of pigs with AB and AA alleles than BB alleles of PRLP genes. Selection for these SNPs could improve the reproductivity in the studied breeds.

4

An Outline of Cardiogenesis

88%

Piotrowski K., Mędrek K., Zajączek S.

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2014

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vol. 4

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issue 1

6-8

EN

The paper presents a description of the development of the human heart based on the present state of knowledge cytogenetics and molecular genetics. Despite the complexity of the genetic mechanisms described, the authors emphasize that it may be just a slice patterns in kardiogenezie. Aberrations and mutations lead to the formation of congenital heart defects in both isolated and components of genetic syndromes.

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The effect of Artichoke (Cynara scolymus L.) on the expression of calcium-binding proteins in the eggshell gland of laying hens

88%

Abadjieva D., Ankova D., Grigorova S., Kistanova E., Abadjieva D., Ankova D., Grigorova S., Kistanova E.

Polish Journal of Veterinary Sciences

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2021

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vol. 24

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issue 1

6

A comparison of a single genetic factor, two stress factors, and one psychosocial coping factor as predictors of depression in an Australian community sample

75%

Sharpley C. F., Palanisamy S. K. A., Metcalf K., Jones K. A., Kelly B., McFarlane J. R.

Archives of Psychiatry and Psychotherapy

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2014

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vol. 16

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issue 4

15-26

EN

Aim. Although both have shown significant effects upon depression in clinical samples, no direct comparison has been reported of the relative power of psychological resilience and the short form of the serotonin transporter gene 5-HTTLPR as predictors of depression in a community sample. material and methods. In a sample set by a priori power analysis, 67 adult females and 59 adult males were used to enable a comparison between a single genetic factor, childhood stressors, recent stressors, psychological resilience and depression. Results. None of genotype, childhood or recent stressors was significantly associated with depression scores, but resilience was a significant inverse predictor of depression scores and also of the presence of clinically significant depression. discussion. These data suggest that measures of an individual's ability to resist or recover from stress may be useful in assessing vulnerability to depression when used with 'at risk' individuals in everyday practice.

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ISOLATION, MOLECULAR CLONING AND CHARACTERISATION OF TWO GENES CODING CHITIN DEACETYLASE FROM MUCOR CIRCINELLOIDES IBT-83

THE GSTP1 c.313A>G POLYMORPHISM MODULATES THE CARDIORESPIRATORY RESPONSE TO AEROBIC TRAINING

Association and polymorphism study of seven candidate genes with reproductive traits in three pig breeds in Hungary

An Outline of Cardiogenesis

The effect of Artichoke (Cynara scolymus L.) on the expression of calcium-binding proteins in the eggshell gland of laying hens

A comparison of a single genetic factor, two stress factors, and one psychosocial coping factor as predictors of depression in an Australian community sample