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Characterization of Catechins from Smilax domingensis Willd. in Cuba

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Soledispa P. A., González J., Cuéllar A., Pérez J., Monan M.

World News of Natural Sciences

2019

vol. 23

297-305

Gas Chromatography coupled with mass spectrometry was applied to study the fragmentation of selected flavonoids from the ethanolic extract of Smilax domingensis Willd., Smilacaceae, known as zarzaparrilla, after Soxhlet extraction during 20 hours. Compounds belonging to one of the major subgroups found in common plants, i.e. flavanols were studied. Following solvent extraction and derivatization using BSTFA, possibly two different metabolites from the same chemical group were characterized in one analytical run: (+)-catechin and (-)-epicatechin. For the very first time, qualitative data on these analytes in the drug were determined after detailed validation of a sensitive, cheap and reliable GC-MS method.

Flavanols from Japanese quince (Chaenomeles japonica) fruit suppress expression of cyclooxygenase-2, metalloproteinase-9, and nuclear factor-kappaB in human colon cancer cells

86%

Owczarek K., Hrabec E., Fichna J., Sosnowska D., Koziołkiewicz M., Szymański J., Lewandowska U.

Acta Biochimica Polonica

2017

vol. 64

issue 3

567-576

Natural polyphenols and polyphenol-rich extracts have been found to possess preventive and therapeutic potential against several types of cancers, including colorectal cancer (CRC), which is an example of an inflammation-associated cancer. This study examines the chemopreventive effect of a Japanese quince (Chaenomeles japonica) fruit flavanol preparation (JQFFP) on colon cancer SW-480 cells. JQFFP, rich in procyanidin monomers and oligomers, was found to inhibit the SW-480 cell viability by 40% at 150 µM catechin equivalents (CE) after 72 h incubation when compared to control, but it was non-toxic to normal colon fibroblast CCD-18Co cells. Furthermore, 100 µM CE JQFFP suppressed COX-2 mRNA expression to 36.7% of control values and protein expression to 77%. In addition, JQFFP reduced the MMP-9 protein expression (to 24% vs. control at 100 µM CE) and caused inhibition of its enzymatic activity (to 35% vs. control at 100 µM CE). Not only did JQFFP inhibit the COX-2 and MMP-9 levels, but it also reduced the NF-κB protein expression (to 65% of control) and phosphorylation of its p65 subunit (to 51%) at 100 µM CE. These results provide the first evidence that JQFFP inhibits COX-2, MMP-9, and NF-κB expression, suggesting that it has cytotoxic, anti-inflammatory, and anti-metastatic activities towards the colon cancer SW-480 cells.

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1 Acta Biochimica Polonica

1 World News of Natural Sciences

1 2019

1 2017

1 Cuéllar A.

1 Fichna J.

1 González J.

1 Hrabec E.

1 Koziołkiewicz M.

1 Lewandowska U.

1 Monan M.

1 Owczarek K.

1 Pérez J.

1 Soledispa P. A.

1 Sosnowska D.

1 Szymański J.

Search results

Characterization of Catechins from Smilax domingensis Willd. in Cuba

Flavanols from Japanese quince (Chaenomeles japonica) fruit suppress expression of cyclooxygenase-2, metalloproteinase-9, and nuclear factor-kappaB in human colon cancer cells