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Non-enzymatic activation of prothrombin induced by interaction with fibrin β26-42 region

100%

Chernyshenko V., Chernyshenko T., Korolova D. y., Volynets G., Kolesnikova I., Platonova T., Lugovskoy E.

Acta Biochimica Polonica

2015

vol. 62

issue 3

517-522

We have discovered that addition of monomeric desAB fibrin to prothrombin leads to appearance of the thrombin-like activity of prothrombin towards S2238 chromogenic substrate. DesA and desABβ(15-42)2 fibrin forms did not cause any activation of prothrombin. From this observation we could suggested that amino acid residues of the 15-42 fragment of BβN-domain presented in desAB fibrin, cleaved in desABβ(15-42)2 fibrin and protected in desA fibrin, play a crucial role in the non-enzymatic activation of prothrombin. To identify the Bβ amino acid residues involved in the fibrin-prothrombin binding we used monoclonal antibodies 1-5G and 2d2a with epitopes in Bβ26-42 and Bβ12-26 fibrin fragments respectively. The thrombin-like activity in the mixture of prothrombin and desAB fibrin was monitored in the presence of each of these monoclonal antibodies. It was found that anti-Bβ12-26 antibody does not exhibit any inhibitory effect on the thombin-like activity of the mixture. In contrast, adding of Bβ26-42 antibody into the mixture of desAB fibrin with prothrombin diminished the thrombin-like activity by 70%. Recombinant dimeric peptides Bβ(15-44)2 and Bβ(15-66)2 that mimic amino acid residues in fibrin were also tested for their ability to activate prothrombin. It was found that both peptides were able to induce non-enzymatic activation of prothrombin. The activation was more evident in the case of Bβ(15-44)2 peptide. From the data obtained we can conclude that desAB fibrin binds to prothrombin through the Bβ26-42 amino acid residues and the formation of such a complex caused a non-enzymatic activation of prothrombin.

Influence of phosphorohydrazone derivatives of benzopyrones on polymerization and viscosity of fibrin

86%

Michalska M., Pająk W., Kołodziejska J., Łazarenkow A., Nawrot-Modranka J.

Acta Biochimica Polonica

2008

vol. 55

issue 3

613-617

The synthesis and antitumour and antibacterial activity of coumarin and chromone phosphorohydrazones have been reported. This study describes influence of phosphorohydrazones derivatives of coumarin and chromone on the polymerization and viscosity of fibrin. The fibrin polymerization assay was performed by the Shen and Lorand method and the clot viscosity was measured on the basis of Shen and Lorand and Marchi and coworkers methods. Among the eight compounds tested, one coumarin derivative and two chromone derivatives showed significant activity.

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2 Acta Biochimica Polonica

1 2015

1 2008

1 Chernyshenko T.

1 Chernyshenko V.

1 Kolesnikova I.

1 Korolova D. y.

1 Kołodziejska J.

1 Lugovskoy E.

1 Michalska M.

1 Nawrot-Modranka J.

1 Pająk W.

1 Platonova T.

1 Volynets G.

1 Łazarenkow A.

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Non-enzymatic activation of prothrombin induced by interaction with fibrin β26-42 region

Influence of phosphorohydrazone derivatives of benzopyrones on polymerization and viscosity of fibrin