Search results

1

Single-walled carbon nanotubes fractionation via electrophoresis

100%

Scheibe B., Lukaszczuk P., Rümmeli M., Kalenczuk R., Borowiak-Palen E.

Polish Journal of Chemical Technology

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2011

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vol. 13

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issue 3

1-4

EN

This work presents the influence of the sonication time on the efficiency of the metallic/semiconducting (M/S) fractionation of diazonium salt functionalized single-walled carbon nanotubes (SWCNTs) via free solution electrophoresis (FSE) method. The SWCNTs synthesized via laser ablation were purified from amorphous carbon and catalyst particles through high vacuum annealing and subsequent refluxing processes in aqua regia solutions, respectively. The purified material was divided into two batches. The SWCNTs samples were dispersed in 1% SDS solution in ultrasound bath for 2 and 12 hours. Both dispersed SWCNTs samples were functionalized with p-aminobenzoic acid diazonium salt and fractionated via free solution electrophoresis method. Afterwards, the fractionated samples were recovered, purified from surfactant/functionalities by annealing and investigated via UV-Vis-NIR optical absorption spectroscopy (OAS). The efficiency of the fractionation process was estimated through the comparison of the van Hove singularities (vHS) presented in the obtained fractions to the starting SWCNTs.

2

Application of electrophoretic methods for detection of protein-porphyrin complexes.

100%

Kowalska A., Kwiek P., Miłosz E., Gondek G., Romiszewska A., Graczyk A., Podhajska A. J.

Acta Biochimica Polonica

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2003

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vol. 50

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issue 4

1155-1163

EN

Simple methods for detection and isolation of protein-porphyrin complexes were elaborated in our laboratory. They are based on the separation of protein-porphyrin complexes in native polyacrylamide gel and measurement of their fluorescence, with the use of two detection systems: the commercially available Gel DocTM 2000 system, and a system specially designed for the purpose of these investigations, concerning protein-porphyrin interactions. The fluorescent complexes can be electro-transferred from the gel onto PVDF membrane, eluted and analyzed in order to identify the protein interacting with porphyrins.

3

Separation of surfactant functionalized single-walled carbon nanotubes via free solution electrophoresis method

100%

Scheibe B., Rümmeli M., Borowiak-Palen E., Kalenczuk R.

|

EN

This work presents the application of the free solution electrophoresis method (FSE) in the metallic / semiconductive (M/S) separation process of the surfactant functionalized single-walled carbon nanotubes (SWCNTs). The SWCNTs synthesized via laser ablation were purified through high vacuum annealing and subsequent refluxing processes in aqua regia solution. The purified and annealed material was divided into six batches. First three batches were dispersed in anionic surfactants: sodium dodecyl sulfate (SDS), sodium cholate (SC) and sodium deoxycholate (DOC). The next three batches were dispersed in cationic surfactants: cetrimonium bromide (CTAB), benzalkonium chloride (BKC) and cetylpyridinium chloride (CPC). All the prepared SWCNTs samples were subjected to FSE separation process. The fractionated samples were recovered from control and electrode areas and annealed in order to remove the adsorbed surfactants on carbon nanotubes (CNTs) surface. The changes of the van Hove singularities (vHS) present in SWCNTs spectra were investigated via UV-Vis-NIR optical absorption spectroscopy (OAS).

4

B-cell chronic lymphocytic leukemia-associated nuclear antigens

100%

Rogalińska M., Błoński J. Z., Robak T., Kiliańska Z. M., Małgorzata Rogalińska - University of Łódź D. o. C., Jerzy Z. Błoński - Medical University of Łódź D. o. H., Tadeusz Robak - Medical University of Łódź D. o. H., Zofia M. Kiliańska - University of Łódź D. o. C.

Acta Universitatis Lodziensis. Folia Biologica et Oecologica

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2008

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vol. 4

EN

One- and two-dimensional polyacrylamide gel electrophoresis were used to compare the composition of nuclear polypeptides from normal and В-cell chronic lymphocytic leukemia mononuclear cells. Against two electrophoretically-specific nuclear proteins with molecular weight of 38/39 and 44/46 kD a from leukemic cells rabbit sera were obtained. As it was analyzed by Western blot technique the available antisera recognized the 38/39 kDa antigen in 53 of the 56 (94.6%), while the 44/46 kDa in 46 of the 49 (93.9%) of examined В-CLL nuclear fraction preparations, but not in normal ones. The pi values of described leukaemia-specific antigens were determined; p38/39 had pi in the range of pH 6.55 -7.00 and p44/46 - in the range of pH 6.2-6.4.

5

Numerical simulation of multiple species detection using hydrodynamic/electrokinetic focusing

88%

Hahm J., Beskok A.

Bulletin of the Polish Academy of Sciences: Technical Sciences

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2005

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vol. 53

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issue 4

6

Techniques of aligning carbon nanotubes

75%

Iakoubovskii K.

Open Physics

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2009

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vol. 7

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issue 4

645-653

EN

This paper reviews major techniques of aligning carbon nanotubes, either during the growth or by the post-growth processing. A number of post-processing alignment techniques are discussed, which employ mechanical stretching, fracture, compression, friction, filtration, fiber drawing, gas flow, liquid crystals, Langmuir-Blodgett technique, acoustic, magnetic and electric fields. The suitability of those techniques to industrial applications is analyzed.

7

Elektroforetyczna identyfikacja proteinaz cysteinowych w surowicach pacjentów z przewlekłą białaczką limfocytową (PBL) z wykorzystaniem biotynylowanego jodoacetamidu

63%

Młudzik P., Pietrzak J., Saed L., Wodziński D., Franiak–Pietryga I., Mirowski M.

Folia Medica Lodziensia

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2016

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vol. 43

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issue 1

35-55

EN

Introduction: Cysteine proteases are enzymes that regulate numerous physiological and pathological processes in the human body. Disorders of their activity can lead to a number of diseases. They play an important role in the process of carcinogenesis, participating in the invasion, transformation, angiogenesis, apoptosis and metastasis. The aim of this study was to elaborate the electrophoretic method of cysteine proteinases identification in the sera of patients suffering from chronic lymphocytic leukemia based on biotinylated iodoacetamide. Material and methods: Preliminary studies were carried out on the commercially available papain (EC 3.4.22.2) well known and widely used plant cysteine protease with a molecular weight 23,4 kDa. The study was conducted on the blood samples taken from patients with chronic lymphocytic leukemia (CLL) and control sera from healthy donors. The sera after the preincubation with iodoacetamide were mixed with the sample buffer followed by electrophoresis on polyacrylamide gel containing sodium dodecyl sulfate (SDS–PAGE). The separated proteins were electrophoretically transferred to the nitrocellulose membranes and subjected to the further analysis using streptavidin conjugated with horseradish peroxidase (HRP). The use of substrate for HRP 3,3’- diaminobenzidine tetrachloride (DAB) allows the biotinylated iodoacetamide and thereby cysteine proteinase identification. Results: The comparative analysis of the sera from the patients with chronic lymphocytic leukemia and the control sera led to the identification of additional protein with a cysteine protease characteristic having a molecular weight of about 37 kDa, which did not occur or was present in a smaller amount of the control sera. Conclusions: The developed method allows the detection of cysteine proteases which are present in the control sera and the sera of patients with chronic lymphocytic leukemia.

Refine search results

Single-walled carbon nanotubes fractionation via electrophoresis

Application of electrophoretic methods for detection of protein-porphyrin complexes.

Separation of surfactant functionalized single-walled carbon nanotubes via free solution electrophoresis method

B-cell chronic lymphocytic leukemia-associated nuclear antigens

Numerical simulation of multiple species detection using hydrodynamic/electrokinetic focusing

Techniques of aligning carbon nanotubes

Elektroforetyczna identyfikacja proteinaz cysteinowych w surowicach pacjentów z przewlekłą białaczką limfocytową (PBL) z wykorzystaniem biotynylowanego jodoacetamidu