Search results

1

Seeding cell number required for optimal lipid accumulation during adipocyte differentiation using 3T3-L1 cell line

100%

Ariyanto E. F., Odaiyappan N. A. L. S., Lidyana L., Wikayani T. P., Qomarilla N., Wira D. W., Triatin R. D.

World News of Natural Sciences

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2019

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vol. 25

220-226

EN

Obesity is one of the major causes of metabolic diseases such as diabetes and heart attack, and, hence, can lead to low quality of life. Elaborating adipocyte differentiation is very crucial for formulating the treatment and prevention of obesity. The objective of this study is to investigate the seeding cell number required to obtain optimum lipid accumulation during adipocyte differentiation using the 3T3-L1 cell line. Two sets of 5.48×104 (for Day 0 and Day 8 of differentiation), of 10.96×104 (for Day 8) and of 21.92×104 (for Day 8) of 3T3-L1 cells were seeded in each wells of a 12-well plate. Isobuthylmethylxanthine (IBMX), Dexamethasone, and Insulin-containing differentiation cocktails was added into the medium at Day 0 for 48 hours. The medium was changed every two days. Day 0 and Day 8 samples were then stained using Oil Red O and were examined under the microscope to observe the lipid droplets (red-coloured). The lipid droplets were quantified by measuring the absorbance at wavelength of 550 nm. In the study, seeding the number of 10.96×104 cells produced very significantly higher lipid accumulation, as compared with seeding the number of 5.48×104 cells. However, doubling the seeding number into 21.92×104 cells did not increase the lipid droplets significantly. This study found that the optimum seeding number to obtain the maximum lipid droplets during 3T3-L1 adipocyte differentiation was 10.96×104 cells.

2

On the relationship between fractal geometry of space and time in which a system of interacting cells exists and dynamics of gene expression.

88%

Waliszewski P., Molski M., Konarski J.

Acta Biochimica Polonica

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2001

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vol. 48

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issue 1

209-220

EN

We report that both space and time, in which a system of interacting cells exists, possess fractal structure. Each single cell of the system can restore the hierarchical organization and dynamic features of the entire tumor. There is a relationship between dynamics of gene expression and connectivity (i.e., interconnectedness which denotes the existence of complex, dynamic relationships in a population of cells leading to the emergence of global features in the system that would never appear in a single cell existing out of the system). Fractal structure emerges owing to non-bijectivity of dynamic cellular network of genes and their regulatory elements. It disappears during tumor progression. This latter state is characterized by damped dynamics of gene expression, loss of connectivity, loss of collectivity (i.e., capability of the interconnected cells to interact in a common mode), and metastatic phenotype. Fractal structure of both space and time is necessary for a cellular system to self-organize. Our findings indicate that results of molecular studies on gene expression should be interpreted in terms of space-time geometry of the cellular system. In particular, the dynamics of gene expression in cancer cells existing in a malignant tumor is not identical with the dynamics of gene expression in the same cells cultured in the monolayer system.

3

Is it possible to differentiate between pseudopneumoperitoneum and similar pathologies ultrasonographically?

88%

Smereczyński A., Kołaczyk K.

Journal of Ultrasonography

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2017

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vol. 17

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issue 68

30-35

EN

Aim: The goal of the work was comparing gas ultrasound images below the right diaphragm in two groups: in people with intestinal interposition below the diaphragm and ones with pneumoperitoneum and extracting the traits differentiating these two conditions. Material and methods: Retrospectively, the documentation of 22 patients with intestinal interposition below the diaphragm (group 1) was utilized. Clinical material was used for comparison, previously published, composed of 15 cases of pneumoperitoneum following laparotomy and of 14 cases following that symptom as a result of ulcer perforation – group 2 (in total n = 29). Moreover, the distance in millimeters of the gas surface reflecting ultrasounds from the parietal peritoneum was measured, the smoothness of the surface, parietal peritoneum enhancement at the place of gas adherence, gas continuity below the diaphragm with gas in the intestine located below the liver. Results: Direct adherence of the gas surface to the diaphragm was observed in 100% of the cases of emphysema, but in no cases of intestinal interposition. Yet, in the group of patients with colonic interposition (n = 21) there was always a small gap (2–3 mm) and the gas surface among those patients in 100% of the cases was uneven. Conclusions: In differentiation between pneumoperitoneum and liverdiaphragm interposition of the intestine one should take into account – apart from gas movement below the diaphragm at body position changing – the presence of protrusion and section enhancement of the diaphragmatic peritoneum as well as the distance of the gas from the diaphragm, the smoothness of its surface and the continuity with the intestine below the liver. Interpositions of small diaphragm-liver penetration may subside in erect position.

PL

Gaz pod przeponą na zdjęciach RTG klatki piersiowej lub jamy brzusznej jest zazwyczaj niepokojącym objawem i przede wszystkim wymaga wykluczenia odmy otrzewnowej. Niekiedy w celu wyjaśnienia tego zjawiska należy wykonać tomografię komputerową, która najlepiej rozstrzyga tę kwestię(1,2). Biorąc pod uwagę wszelkie zalety ultrasonografii, w tym badanie w przypadkach nagłych i w ekstremalnie trudnych warunkach, warto określić, czy metoda ta umożliwia odróżnienie odmy otrzewnowej od stanów chorobowych ją pozorujących, czyli pneumoperitoneum od pseudopneumoperitoneum(3–5). Pod tym ostatnim terminem kryje się głównie nietypowe położenie jelita między przeponą a wątrobą, co w piśmiennictwie określa się objawem Chilaiditiego (od nazwiska autora pierwszej publikacji na ten temat). W symptomatologii kliniczno-radiologicznej wyróżnia się ponadto zespół Chilaiditiego, który wskazuje na istnienie związku przyczynowego między istniejącą interpozycją przeponowo-wątrobową jelita a różnymi dolegliwo- ściami ze strony przewodu pokarmowego, układu oddechowego lub układu krążenia(1,2,6–10). Ultrasonografia jest rzadko wykorzystywana w różnicowaniu odmy otrzewnowej z objawem Chilaiditiego(4,11–15). W celu poszerzenia tej diagnostyki retrospektywnie zanalizowano 22 przypadki z interpozycją jelita pod przeponą i porównano z wcześniej uzyskanymi danymi w przypadkach z odmą otrzewnową samoistną oraz po laparotomiach (łącznie n = 29)(16–18). Artykuł w wersji polskojęzycznej jest dostępny na stronie http://jultrason.pl/wydawnictwa/volume-17-no-68

4

Changes in the cellular behaviour of human colonic cell line Caco-2 in response to butyrate treatment.

75%

Dzierżewicz Z., Orchel A., Węglarz L., Latocha M., Wilczok T.

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vol. 49

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issue 1

211-220

EN

Gut-derived adenocarcinoma Caco-2 cells were treated with sodium butyrate (NaB) at physiologically relevant concentrations. We characterized its effects on proliferation, differentiation, apoptosis, adhesion to the solid support and interleukin-8 secretion. Differentiation was determined by brush border alkaline phosphatase activity. Apoptosis was assessed by acridine orange and Hoechst stains. Differentiation and apoptosis were analyzed in both adherent and floating cell populations. The transformed Caco-2 cells did not retain their malignant phenotype in the presence of NaB. They appeared to undergo a change in the phenotype induced by NaB, as indicated by reduced proliferation, enhanced differentiation, stimulation of apoptosis leading to decreased viability of cells, and stimulation of interleukin-8 secretion. Considering all the above facts and data, we postulate that Caco-2 cells cultured in NaB supplemented medium could regain the phenotypic characteristics of the phenotype of the parent cell from which originated the Caco-2 line.

5

Combined effects of doxorubicin and STI571 on growth, differentiation and apoptosis of CML cell line K562

75%

Jakubowska J., Stasiak M., Szulawska A., Bednarek A., Czyz M.

Acta Biochimica Polonica

|

2007

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vol. 54

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issue 4

839-846

EN

STI571 (imatinib mesylate; Gleevec®) is an inhibitor that targets the tyrosine kinase activity of Bcr-Abl present in chronic myelogenous leukemia (CML) cells. Some preclinical studies have demonstrated that the combination of STI571 with chemotherapeutic drugs results in enhanced toxicity in Bcr-Abl-positive leukemias. We investigated the potential benefit of using STI571 to down-regulate Bcr-Abl activity for the enhancement of doxorubicin anti-proliferative action in K562 cell line derived from blast crisis of CML. At low concentrations of both drugs (40 nM doxorubicin combined with STI571 in the range of 100-150 nM), the antiproliferative effects were mainly due to cellular differentiation as assessed by benzidine staining for hemoglobin synthesis level and real-time PCR for γ-globin expression. Higher concentrations of STI571 used in combinations with doxorubicin caused mainly apoptosis as shown by DNA degradation and nuclear fragmentation visualized by fluorescence microscopy after DAPI staining, changes in cell morphology observed after Giemza-May Grünwald staining and cellular membrane organization estimated by flow cytometry after Annexin V staining. As compared with either drug alone, cotreatment with STI571 and DOX induced stronger cellular responses. A low concentration of STI571 in combination with a low concentration of DOX might be tested as an alternative approach to increasing the efficacy of chemotherapy against CML.

6

Detection and differentiation of Newcastle disease virus and influenza virus by using duplex real-time PCR

75%

Nidzworski D., Wasilewska E., Smietanka K., Szewczyk B., Minta Z.

Acta Biochimica Polonica

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2013

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vol. 60

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issue 3

475-480

EN

Newcastle disease virus (NDV), member of the Paramyxoviridae family and avian influenza virus (AIV), member of the Orthomyxoviridae family, are two main avian pathogens causing serious economic problems in poultry health. Both are enveloped, single-stranded, negative-sense RNA viruses and cause similar symptoms, ranging from sub-clinical infections to severe diseases, including decrease in egg production, acute respiratory syndrome, and high mortality. Similar symptoms hinder the differentiation of infection with the two viruses by standard veterinary procedures like clinical examination or necropsy. To overcome this problem, we have developed a new duplex real-time PCR assay for the detection and differentiation of these two viruses. Eighteen NDV strains, fourteen AIV strains, and twelve other (negative control) strains viruses were isolated from allantoic fluids of specific pathogen-free (SPF), embryonated eggs. Four-weeks-old SPF chickens were co-infected with both viruses (NDV - LaSota and AIV - H7N1). Swabs from cloaca and trachea were collected and examined. The results obtained in this study show that by using duplex real-time PCR, it was possible to detect and distinguish both viruses within less than three hours and with high sensitivity, even in case a bird was co-infected. Additionally, the results show the applicability of the real-time PCR assay in laboratory practice for the identification and differentiation of Newcastle disease and influenza A viruses in birds.

7

Developmental and Cytochemical Studies of the Endosperm Chalazal Haustorium of Rhinanthus Serotinus (Scrophulariaceae)

75%

Świerczyńska J., Kozieradzka-Kiszkurno M., Bohdanowicz J.

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vol. 55

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issue 1

8

Proliferation and Ability for Epidermal Autoregeneration in Patients with Chronic Lower Leg Venous Ulcerations

75%

Waligóra J., Noszczyk B.

Polish Journal of Surgery

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2007

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vol. 79

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issue 2

113-119

EN

The protein p63 plays a significant role in the development of animal epithelium. p63 is a regulator of differentiation, senescence and adhesion programs in numerous mature epithelial tissues. In patients with a healthy epidermis, p63 maintains cell progenitor potential - the ability for cellular division to occur using the delayed differentiation program. It is also responsible for the protecting the epithelial phenotype from depletion in migrating cells, thus resulting in invasion and infiltration after altering its endogenous expression.The aim of the study was to compare the number of cells with p63 protein expression and the presence of Ki67 proliferation marker in the epidermis in patients with chronic venous ulcerations versus those with properly healing wounds.Material and methods. Study materials were comprised of biopsy samples collected from healthy volunteers and patients treated for venous ulcerations. The specimens were subjected to immunohistochemical staining using available monoclonal antibodies and were analyzed with an imaging analysis program which evaluated the expression indices of both proteins in areas of intensified cellular division, i.e. wound edges.Results. The number of cells displaying protein expression in patients with chronic venous ulcerations was significantly lower in comparison to the values observed in healthy volunteers. This was determined during the intermediary phase of wound healing, the most pronounced phase of cellular response to injury.Conclusions. Decreased epidermal p63 expression in patients with venous ulcerations suggests insufficient protein production for the maintenance of autoregeneration and long-lasting division; both are required for the supplementation of migrating cells. The above-mentioned phenomenon suggests that there may be a role for p63 in regulation of the healing process and pathophysiology of chronic venous leg ulcerations.

9

Development of a recombinant NP protein based indirect ELISA for the detection of antibodies against Newcastle disease virus and differentiation of infected or recombinant vaccine immunized chickens

75%

Domańska-Blicharz K., Tyborowska J., Sajewicz-Krukowska J., Olszewska-Tomczyk M., Rąbalski Ł., Kucharczyk K., Szewczyk B., Śmietanka K., Domańska-Blicharz K., Tyborowska J., Sajewicz-Krukowska J., Olszewska-Tomczyk M., Rąbalski Ł., Kucharczyk K., Szewczyk B., Śmietanka K.

Polish Journal of Veterinary Sciences

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2019

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issue 3

10

Zastosowanie komórek macierzystych w leczeniu pacjentów z chorobą Parkinsona

63%

Rieske P.

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issue 4

232-241

EN

This review shows current progress in stem cell therapy of Parkinson disease. Article depicts strategies of stem cell therapy, discusses results of trials performed to treat Parkinson disease, describes experience of author in preparing cells for patients with Parkinson disease, and presents potential danger of stem cell therapy. Several strategies of stem cell therapies are presented. Strategies are divided in to: physiological, physiologicallybiotechnological and biotechnological. Physiological strategy includes: use of neural stem cells, and Ratajczak concept. Plasticity (transdifferentiation), is considered as approach physiologically-biotechnological. Biotechnological strategy includes: cloning and reprogramming. Article shows opinions of authorities working on Parkinson disease stem cells therapy, briefly discusses trials of Hauser et al., Hegel et al., Brundin et al., Freed et al., and Olanow et al. Clues coming from these trials, for future use of stem cells derivatives, in the treatment of Parkinson disease are presented. Hints coming from works on Parkinson disease animals models, are also included. Moreover author presents his own experience in preparing cells potentially useful in Parkinson disease treatment, which is use of fibroblasts and neural stem cells. Finally possible dangerous consequences of stem cells therapy, such as risk of cancers development, are shown. Stem cell therapy appears as progressing, but in author opinion there is no final conclusion to say if it will work very efficiently or not.

PL

W ostatnim czasie dokonał się istotny postęp w terapii komórkowej choroby Parkinsona. W niniejszym artykule przedstawiono strategie dotyczące terapii komórkowej, opisano wyniki prób klinicznych, przedstawiono także doświadczenia autora w przygotowaniu komórek dla osób z chorobą Parkinsona oraz uwypuklono niebezpieczeństwa, jakie mogą wynikać ze stosowania terapii komórkowej. Zaprezentowano takie strategie terapii komórkowej, jak: strategia fizjologiczna, strategia fizjologiczno-biotechnologiczna i strategia biotechnologiczna. W ramach strategii fizjologicznej ujęto zastosowanie neuralnych komórek macierzystych i koncepcję Ratajczaka. Plastyczność (transróżnicowanie) jest rozpatrywana jako podstawa strategii fizjologiczno-biotechnologicznej. Strategia biotechnologiczna to klonowanie i reprogramowanie. W artykule zaprezentowano także opinie autorytetów na temat skuteczności terapii komórkowej w leczeniu pacjentów z chorobą Parkinsona oraz krótko opisano próby kliniczne, którymi niezależnie od siebie kierowali: Hauser, Hegel, Brundin, Freed i Olanow. Przedstawiono również implikacje ich dokonań badawczych dla przyszłych prób wykorzystania komórek macierzystych w terapii osób z chorobą Parkinsona, jak również wyniki badań nad zwierzętami z eksperymentalnie wywołanym parkinsonizmem. Autor prezentuje ponadto wyniki własnych doświadczeń w przygotowywaniu komórek potencjalnie użytecznych w leczeniu pacjentów z chorobą Parkinsona oraz omawia istotne zagrożenia związane ze stosowaniem terapii komórkowej (takie jak choroba nowotworowa). Terapia komórkowa rozwija się, jednak w odczuciu autora niniejszego artykułu nie ma pewności, czy będzie ona naprawdę skuteczną.

11

Sport and Globalisation: Homogenization and Differentiation

63%

Gomez R.

Physical Culture and Sport. Studies and Research

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2009

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vol. 47

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issue 1

20-27

EN

The development of the worldwide market has motivated long-ranging consequences, not only at the level of growing economic interdependencies, but also in the globalization of cultures and lifestyles. At any of these dimensions, sport plays a role and contributes in its own particular way to globalization. Transnational organizations, worldwide events, transnational communities and transnational structures organised around the central theme of Sport provide good evidence of that phenomenon. However, the way these dimensions interrelate at a time of unorganised capitalism is based on disjuncture. Following this thesis, Appadurai (1996) has proposed an elementary scheme for the analysis of the disjuncture between the several dimensions of globalization, suggesting the notion of landscapes to underline the fluid and irregular shape of the capital flow, pertaining to both communications and lifestyles. By emphasising that globalization is intensively perceived according to, and influenced by the historical, linguistic and political contexts of the intervening players, the author deliberately focuses on the imagined worlds that help us construct those landscapes. In this paper, we will retrieve some of those theoretical leads and analyse three types of landscape in the leisure and sports contexts, in an attempt to demonstrate how their interrelation is one of disjuncture, where some dimensions promote sports homogenization while others push towards increasing differentiation. We will analyse the mediascapes (Sport as global spectacle), the technoscapes (the role of the new media and velocity in the creation of decontextualised global cognitive maps), and the ideoscapes (the role of images and the aesthetisation of the leisure sports experiences)

12

Rewolucja w biologii rozwojowej

63%

Rieske P.

Aktualności Neurologiczne

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2006

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vol. 6

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issue 3

159-163

EN

During the last decade dozens of papers have been published, whose approval for printing signifies a consent for changes in some biology handbooks. Stem cells have found their way to newspaper headlines and even became an element of presidential campaign in the United States. Indeed, an important issue on scientific and medical ground is that recently several paradigms in hitherto force in developmental biology have been challenged. First, the paradigm of barrier between embryonal layers has been questioned. Second, it has been demonstrated that even in such structures as the central nervous system and heart muscle there is a steady but distinct renovation, even in adult mammals. Third, new models of cell differentiation have been proposed. For many years, developmental biology was based on instructional models of differentiation. At present, the instructional model is increasingly frequently replaced by the stochastic model. Furthermore, the question of debate is whether there is still any rationale for further propagation of hierarchic models. Current dispute concerning the above mentioned concepts and paradigms, which is currently taking place among biologists studying the development of organisms, has a direct transmission onto practical applications thereof. For example, lack of barrier between embryonal layers (or its permeability) paves the way for the use of mesenchymal stem cells from bone marrow as “progenitors” of neurons, whose deficit is seen in persons with Parkinson disease. In other words, if inter-embryonal layer barrier does not exist or may be transgressed, then therapeutic cloning may be substituted by simple aspiration of bone marrow or sampling of skin fibroblasts. After a few years of negation, it is accepted again that in mammals take place processes of dedifferentiation and transdifferentiation. This paper presents old paradigms as well as arguments of partisans of challenging or even to refute these paradigms.

PL

W ostatnim dziesięcioleciu opublikowano dziesiątki artykułów, których zaakceptowanie oznacza zgodę na dokonanie zmian w niektórych podręcznikach biologii. Komórki macierzyste „trafiły na pierwsze strony gazet” i stały się nawet przedmiotem kampanii prezydenckiej w Stanach Zjednoczonych. Co jednak istotne, z naukowego i medycznego punku widzenia w ostatnich latach podważonych zostało kilka paradygmatów obowiązujących dotychczas w biologii rozwojowej. Po pierwsze podważono paradygmat istnienia bariery listków zarodkowych. Po drugie stwierdzono, że nawet w obrębie takich struktur, jak ośrodkowy układ nerwowy (OUN) i mięsień sercowy, następuje powolna, ale jednak wyraźna odnowa u dorosłych ssaków. Po trzecie zaproponowano nowe modele różnicowania komórek. Przez wiele lat dominowały w biologii rozwojowej instrukcyjne modele różnicowania. W tej chwili w miejsce modelu instrukcyjnego coraz częściej wprowadza się model stochastyczny. Oprócz tego rozważa się, czy ma uzasadnienie dalsze propagowanie tzw. modeli hierarchicznych. Spór dotyczący wymienionych pojęć i paradygmatów, który toczy się wśród biologów zajmujących się rozwojem organizmów, przekłada się bezpośrednio na działania praktyczne. Przykładowo, brak bariery listków zarodkowych (lub jej nieszczelność) otwiera drogę do wykorzystania na przykład mezenchymalnych komórek macierzystych ze szpiku kostnego jako „progenitorów” neuronów, których niedobór obserwujemy u osób z chorobą Parkinsona. Innymi słowy, jeżeli bariera listków zarodkowych nie istnieje lub może być przekroczona, klonowanie terapeutyczne można zastąpić zwykłą aspiracją szpiku kostnego lub pobraniem fibroblastów skóry. Ponownie przyjęto – po kilku latach negowania tego faktu – iż w organizmach ssaków zachodzą procesy odróżnicowania (dedyferencjacji) i transróżnicowania (transdyferencjacji). W artykule przedstawiono stare paradygmaty, jak również argumenty zwolenników podważenia lub obalenia tychże paradygmatów.

13

Białko cacybp/sip i jego rola w organizacji cytoszkieletu

51%

Filipek A., Góral A.

Kosmos

|

2018

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vol. 67

|

issue 1

131-137

PL

Białko CacyBP/SIP występuje w różnych komórkach i tkankach ssaków, a jego wysoki poziom notowany jest w mózgu, śledzionie, grasicy oraz w wielu nowotworach. CacyBP/SIP oddziałuje z wieloma białkami efektorowymi, w tym z białkami cytoszkieletu: aktyną, tubuliną, tropomiozyną. Wskazuje to, iż CacyBP/SIP bierze udział w procesach komórkowych, którym towarzyszą zmiany w organizacji cytoszkieletu zarówno w warunkach fizjologicznych jak też w różnych stanach chorobowych. W niniejszej pracy przedstawiono charakterystykę oddziaływania CacyBP/SIP z białkami cytoszkieletu oraz rolę tych interakcji w różnych procesach komórkowych.

EN

The CacyBP/SIP protein is present in different mammalian cells and tissues. Its particularly high level is observed in brain, spleen, thymus and in many cancers. CacyBP/SIP interacts with different targets including cytoskeletal proteins such as tubulin, actin, tropomyosin. This indicates that CacyBP/SIP is involved in cellular processes associated with changes in cytoskeleton organization under physiology and pathology. In the present work the characteristics of complexes formed between CacyBP/SIP and cytoskeletal proteins and the role of those interactions are presented.

14

Side-chain modified vitamin D analogs require activation of both PI 3-K and erk1,2 signal transduction pathways to induce differentiation of human promyelocytic leukemia cells.

51%

Marcinkowska E., Kutner A.

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vol. 49

|

issue 2

393-406

EN

Synthetic analogs of vitamin D for potential use in differentiation therapy should selectively regulate genes necessary for differentiation without inducing any perturbations in calcium homeostasis. PRI-1906, an analog of vitamin D2, and PRI-2191, an analog of vitamin D3 bind nuclear vitamin D receptor (nVDR) with substantially lower affinity than 1,25-dihydroxyvitamin D3 (1,25-D3), but have higher differentiation-inducing activity as estimated in HL-60 leukemia cell model. To examine how their increased differentiation-inducing activity is regulated we tested the hypothesis that membrane-mediated events, unrelated to nVDR, take part in the differentiation in response to PRI-1906 and PRI-2191. The induction of leukemia cell differentiation in response to the analogs of vitamin D was inhibited by LY294002 (phosphatidylinositol 3-kinase inhibitor), PD98059 (inhibitor of MEK1,2, an upstream regulator of extracellular-signal regulated kinase) and rapamycin (p70S6K inhibitor) pointing out that activation of signal transduction pathways unrelated to nVDR is necessary for differentiation. On the other hand, inhibition of cytosolic phospholipase A2 accelerated the differentiation of HL-60 cells induced by either 1,25-D3 or by the vitamin D analogs suggesting possible existence of a feedback loop between extracellular-signal regulated kinases and phospholipase A2.

Refine search results

Seeding cell number required for optimal lipid accumulation during adipocyte differentiation using 3T3-L1 cell line

On the relationship between fractal geometry of space and time in which a system of interacting cells exists and dynamics of gene expression.

Is it possible to differentiate between pseudopneumoperitoneum and similar pathologies ultrasonographically?

Changes in the cellular behaviour of human colonic cell line Caco-2 in response to butyrate treatment.

Combined effects of doxorubicin and STI571 on growth, differentiation and apoptosis of CML cell line K562

Detection and differentiation of Newcastle disease virus and influenza virus by using duplex real-time PCR

Developmental and Cytochemical Studies of the Endosperm Chalazal Haustorium of Rhinanthus Serotinus (Scrophulariaceae)

Proliferation and Ability for Epidermal Autoregeneration in Patients with Chronic Lower Leg Venous Ulcerations

Development of a recombinant NP protein based indirect ELISA for the detection of antibodies against Newcastle disease virus and differentiation of infected or recombinant vaccine immunized chickens

Zastosowanie komórek macierzystych w leczeniu pacjentów z chorobą Parkinsona

Sport and Globalisation: Homogenization and Differentiation

Rewolucja w biologii rozwojowej

Białko cacybp/sip i jego rola w organizacji cytoszkieletu

Side-chain modified vitamin D analogs require activation of both PI 3-K and erk1,2 signal transduction pathways to induce differentiation of human promyelocytic leukemia cells.