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EN
Water satisfies human needs in many ways. Without water, life is not possible in this planet. According to the estimation of the WHO, 4,000 children die every day from water-borne diseases and this condition is worst in developing and under-developed countries. Water-borne diseases are caused by the consumption of contaminated water. Indeed, it is the main agent of transference of the pathogens causing these diseases. The main objective of this paper is ‘to identify the water-borne diseases and their challenges and to suggest remedial measures to overcome the challenges in the study area’. Both primary and secondary data were used to conduct this study and the analysis of this study is tabulated clearly in the data analysis part. The coastal areas of Ampara District (Sainthamaruthu, Karaitivu, Maligaikadu Mawadipalli, Akkaraipattu, Irakkamam, Varipathanchenai, Addalaichenai, Palamunai, Oluvil and Nintavur) face a lot of challenges induced by water-borne diseases. Viruses, bacteria, parasites and protozoa are the main pathogens. Bacillary dysentery (shigellosis), cholera, other diarrheal diseases, hepatitis A and E and typhoid fever were identified as the most common diseases in these areas. The causes for the diseases are the contamination of water by improper drainage management, effluent contamination of water sources, over population and the lack of awareness among residents about water-borne diseases. According to the result of this study, controlling water contamination, constructing water purification systems, mending existing drainage systems, following instructions when constructing latrines, examining and remediating contaminated wells and providing awareness among residents are ways to attain the objectives.
EN
Energy drinks (EDs) are caffeinated drinks marketed as energy and performance boosters and commonly used by athletes worldwide. They are widely used among youth and university students, with limited research about their consumption among physically active persons. Objectives: This study aims to assess ED consumption among physically active persons in Lebanon, the association between ED use and the participants’ general characteristics, and to explore the predictors of ED use among them. Methods: An observational cross-sectional study was conducted over three months, targeting 384 physically active persons from 8 gyms across Lebanon. Results: The prevalence of ED consumption was 49.2%, namely to boost energy (68.2%) and stimulate awakeness (19.3%). Around 20% used EDs during workouts, and 15.8% used them before it. Females had 59% lower odds of ED consumption than males (OR 0.41, 95% CI 0.19-0.89), while older participants had 4.74 times higher odds of ED use (OR 4.74, 95% CI 1.16-19.3). Waterpipe smokers and alcohol consumption had 3.68 and 2.28 times higher odds of ED use than non-users. Among other sports, those doing weightlifting had significantly higher odds of ED consumption than others (OR 2.61, 95% CI 1.30-5.25). Conclusion: The predictors of ED consumption should be considered for better-informed decisions and effective awareness campaigns.
EN
Impairments in mitochondrial function have been proposed to play an important role in the pathogenesis of diabetes. Atherosclerotic coronary artery disease (CAD) is the leading cause of mortality in diabetic patients. Mitochondrial dysfunction and increased production of reactive oxygen species (ROS) are associated with diabetes and CAD. Elevated levels of glycated low density lipoproteins (glyLDL) and oxidized LDL (oxLDL) were detected in patients with diabetes. Our previous studies demonstrated that oxLDL and glyLDL increased the generation of ROS and altered the activities of antioxidant enzymes in vascular endothelial cells (EC). The present study examined the effects of glyLDL and oxLDL on mitochondrial respiration, membrane potential and the activities and proteins of key enzymes in mitochondrial electron transport chain (mETC) in cultured porcine aortic EC (PAEC). The results demonstrated that glyLDL or oxLDL significantly reduced oxygen consumption in Complex I, II/III and IV of mETC in PAEC compared to LDL or vehicle control using oxygraphy. Incubation with glyLDL or oxLDL significantly reduced mitochondrial membrane potential, the activities of mitochondrial ETC enzymes - NADH dehydrogenase (Complex I), succinate cytochrome c reductase (Complex II + III), ubiquinol cytochrome c reductase (Complex III), and cytochrome c oxidase (Complex IV) in PAEC compared to LDL or control. Treatment with oxLDL or glyLDL reduced the abundance of subunits of Complex I, ND1 and ND6 in PAEC. However, the effects of oxLDL on mitochondrial activity and proteins were not significantly different from glyLDL. The findings suggest that the glyLDL or oxLDL impairs mitochondrial respiration, as a result from the reduction of the abundance of several key enzymes in mitochondria of vascular EC, which potentially may lead to oxidative stress in vascular EC, and the development of diabetic vascular complications.
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