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Pro-inflammatory properties of cadmium

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EN
Cadmium is a toxic and carcinogenic heavy metal that nowadays constitutes a serious environmental health problem. The aim of this study is to review the effects of cadmium on selected inflammatory mediators and markers, such as NF-κB and AP-1 transcription factors, IL-6, TNF-α, IL-1β cytokines, IL-8 or MIP-2 chemokine, MPO, iNOS, MMPs and COX-2 enzymes, PGE2 (product of COX-2 enzyme), ICAM-1, VCAM-1 and PECAM-1 adhesion molecules, and CRP. The research strategy identified articles available in Medline, published between 1998 and 2012; we included both in vivo and in vitro studies carried out on humans and rodents. Most of the reviewed research findings suggest that cadmium in micromolar concentrations (especially in the 1-10 μM range) causes up-regulation of the mediators and markers of inflammation, and appears to have pro-inflammatory properties. However, it is worth mentioning that a contradictory or even opposite hypothesis exists, which suggests cadmium to be an anti-inflammatory factor. Further research including detailed histological analyses should solve this discrepancy. Nevertheless, it appears that the main reason for these contradictory findings is the experimental setup: different biological systems analyzed and different doses of cadmium applied.
EN
In Escherichia coli, heterologous production of Schizosaccharomyces pombe phytochelatin synthase (PCS) along with overproduction of E. coli serine acetyltransferase (SAT) and γ-glutamylcysteine synthase (γECS) was achieved and resulted in the accumulation of phytochelatins in bacterial cells. Overproduction of either γECS alone or simultaneous production of all three proteins in bacterial cells were accompanied by reduced growth rate in liquid cultures. Interestingly, bacteria overproducing either γECS or both SAT and γECS (with elevated level of γ-glutamylcysteine but not of phytochelatins) were able to accumulate more cadmium per dry weight than the control. However, the most efficient cadmium accumulation was observed in bacteria with elevated levels of all three proteins: SAT, γECS and PCS. Therefore, "pushing" the entire pathway might be the most promising approach in modification of bacteria for potential bioremediation purposes because the level of intermediates, cysteine and glutathione, can limit the rate of production of phytochelatins. However, in such bacteria other metabolic process might become limiting for efficient growth.
EN
Background: Osteoporosis is a growing health concern across the world. Some epidemiological data suggest that cadmium increases risk for development of osteoporosis and lead to higher rate of fracture incidents even on low environmental exposure level. Material and methods: Cadmium in urine and bone resorption markers – total fraction of the urinary pyridynoline (Pyr) and deoxypyridinoline (DPyr) – were determined in 36 patients, who were examined for toxic effects of cadmium exposure. Additionally calcium in urine was determined. Associations between cadmium exposure and factors related to bone metabolism were estimated and Pyr and DPyr excretion were compared in three groups categorized across cadmium concentrations. Results: In the investigated group there were significant positive correlations between cadmium levels in urine and Pyr and DPyr excretion. None of the other variables correlated significantly with examined bone resorption markers excluding calcium excretion in urine. Excretion of Pyr and DPyr differed significantly between group with the lowest cadmium concentration (<1.2 μg/g creatinine) and group with the highest cadmium concentration (>1.9 μg/g creatinine), where median values of Pyr and DPyr increased by 49.8% and 37.5%, respectively. Conclusion: The results suggest that cadmium increases bone resorption processes and induce osteotoxic effects in environmental exposure level.
PL
Wstęp: Osteoporoza jest rosnącym problemem na świecie. Niektóre badania epidemiologiczne sugerują, że kadm zwiększa ryzyko rozwoju tej choroby i prowadzi do zwiększenia ryzyka złamań kości nawet przy niskim narażeniu środowiskowym. Materiał i metody: Kadm w moczu oraz biomarkery resorpcji kostnej – całkowite frakcje pirydynoliny (Pyr) i dezoksypirydynoliny (DPyr) w moczu – były oznaczane u 36 pacjentów badanych pod kątem toksycznego działania kadmu. Dodatkowo oznaczano zawartość wapnia w moczu. Oszacowano zależności pomiędzy narażeniem na kadm a czynnikami powiązanymi z metabolizmem kości oraz porównano stężenia Pyr oraz DPyr pomiędzy grupami skategoryzowanymi względem stężenia kadmu w moczu. Wyniki: W badanej grupie wykazano statystycznie istotną pozytywną korelację pomiędzy poziomem kadmu w moczu, a ilością wydalanej Pyr i DPyr z moczem. Żadna z innych badanych zmiennych nie korelowała znacząco z markerami resorpcji kości oprócz wydalania wapnia z moczem. Zróżnicowanie wydalania Pyr i DPyr było statystycznie istotne pomiędzy grupami o najniższym (<1.2 μg/g kreatyniny) i najwyższym stężeniu kadmu w moczu (>1.9 μg/g kreatyniny), w której mediany wartości stężeń Pyr i DPyr wzrosły odpowiednio o 49,8% i 37,5%. Wnioski: Wyniki badań sugerują, że kadm wzmaga procesy resorpcji kości i działa osteotoksycznie również w narażeniu środowiskowym.
EN
Cadmium is a dangerous occupational and environmental toxin. It accumulates in the human organism mainly in liver and kidneys. Cadmium half-life is about 10 years, so the symptoms of cadmium intoxication may occur several years after the exposure. Until now in treating intoxication with this metal chelating compounds have been used, burdened with numerous undesirable symptoms. In our investigations anthocyanins from Aronia melanocarpa were used to reduce the harmful results caused by cadmium. Administering anthocyanins with cadmium chloride resulted in a statistically significant decrease of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activity, concentration of bilirubin and urea in blood serum and decreased cadmium cumulation in liver and kidneys in relation to animals receiving cadmium chloride only.
EN
The presence of toxic heavy metals in industrial wastewater is a serious pollution problem. The ability of chitosan membranes as an adsorbent for cadmium ions in aqueous solution was studied. Experiments were carried out as function of contact time and concentration of cadmium ions. This study has shown that chitosan is capable of removing cadmium from aqueous solution. The removal efficiency depends on reaction time and concentration of cadmium. The Langmuir and Freundlich adsorption models were applied to describe the isotherms and isotherm constants. Equilibrium data agreed very well with the Langmuir model. The maximum adsorption capacity of the Langmuir isotherm equation was 94 mg/g and the Langmuir adsorption equilibrium constant was 1.6 × 10-3 dm3/mg at 25 °C.
EN
The toxic action of cadmium in the bone tissue is known, but its mechanisms are still unexplained. We examined whether Cd influences collagen content and its solubility in the femoral bone of three-week-old female rats exposed to 5 or 50 mg Cd/l in drinking water. Non-cross linked collagen was extracted with 0.5 M acetic acid, and two acid-insoluble collagen fractions were extracted with pepsin and 4.0 M guanidine hydrochloride, respectively. SDS/PAGE showed the presence of two collagen types, I and V, in all three extracted fractions. Exposure of rats to Cd for 6 months increased the amount of acid-soluble collagens type I and V and decreased the level of acid-insoluble collagens. The amount of total collagen extracted from the bones of rats exposed to 50 mg Cd/l was reduced by about 14% as compared to control and those intoxicated with 5 mg Cd/l. The solubility of type I bone collagen (determined as the percentage of acetic-soluble fraction of total collagen) was increased 2.9- and 3.0-fold in rats intoxicated with 5 and 50 mg Cd/l, respectively. Similarly, the solubility of type V collagen was increased 2.3- and 2.7-fold, respectively. Our results indicate that Cd treatment affects bone collagen by decreasing its content and increasing its solubility.
EN
Cadmium is a toxic heavy metal which can cause numerous alterations in cell functioning. Exposure to cadmium leads to generation of reactive oxygen species, disorders in membrane structure and functioning, inhibition of respiration, disturbances in ion homeostasis, perturbations in cell division, and initiation of apoptosis and necrosis. This heavy metal is considered a carcinogen by the Agency for Toxic Substances and Disease Registry. At least some of the described toxic effects could result from the ability of cadmium to mimic other divalent ions and alert signal transduction networks. This review describes the role of cadmium mimicry in its uptake, reactive oxygen species generation, alterations in calmodulin, Wnt/β-catenin and estrogen signaling pathways, and modulation of neurotransmission. The last section is dedicated to the single known case of a favorable function performed by cadmium mimicry: marine diatoms, which live in zinc deficient conditions, utilize cadmium as a cofactor in carbonic anhydrase - so far the only described cadmium enzyme.
EN
The structure and the biosorption properties of fungal biomass of Aspergillus niger originated from citric acid fermentation industry was investigated. This waste biomass, produced in high quantity in carefully controlled industrial processes, has certain favourable characteristics that may be improved for its usefulness. In environmental chemistry, it is known for the removal of heavy metals cations. In this work, different alkaline treatments (1M NaOH/20°C/24 h and 10M NaOH/107°C/6 h) were used to evaluate the dependence of sorption properties of biomass on the cell wall composition. The biosorption was studied by the batch method, with the biomass concentration of 1 g/l, at pH 6. The adsorption of lead was more effective than that of cadmium. The biosorption capacity was evaluated using the biosorption isotherm derived from the equilibrium data. At pH 6, the maximmum lead biosorption capacity estimated with the Langmuir model was 93 mg/g dry biomass.
EN
In the current study, we examined the effects of Cd on Cd, Cu, Zn and Fe levels in placenta and maternal and fetal plasma and tissues, the placental weight, total fetal and maternal body weights, and fetal and maternal tissue weights during pregnancy. A total of 21 adult female rats were treated during gestation with drinking water containing one of the following: 70 mg/L of CdCl2, a combination of 70 mg/L of CdCl2 and 70 mg/L of CuSO4, or no addition (control). Placenta Cu and Fe levels, fetal liver and kidney Cu levels, and fetal liver tissue weights were lower in the group administered Cd than in the control group. Also, Cd levels in the placenta, maternal and fetal liver, and maternal kidney were higher in the group treated with Cd than in controls. In the group administered both Cd and Cu, fetal body and tissue weights did not change, but Cd levels in the placenta, maternal and fetal liver, and maternal kidneys were higher than in controls. Zn and Fe levels in the maternal kidney and fetal liver were also lower in this group. Cd exposure during pregnancy resulted in Cd accumulation in maternal and fetal tissues during pregnancy and a decrease in the total weight of fetuses, and the combination of Cd and Cu caused some changes in the both maternal and fetal levels of Cu, Zn, and Fe, but it did not cause changes in the total fetal body weight or the weights of individual tissues.
EN
In contrast to mRNAs, ribosomal RNAs are generally not considered to be polyadenylated. Only a few recent reports describe non-abundant polyadenylated rRNA-related transcripts that have been detected and characterized in yeast and in human cells. Here we depict the phenomenon of 26S rRNA polyadenylation and degradation that was observed in shoots of Nicotiana tabaccum plants grown in the presence of cadmium. Fragments corresponding to 26S rRNA were identified using suppression subtractive hybridization during screening for genes induced in tobacco plants upon a three-week exposure to 15 µM cadmium chloride. Extracts prepared from the above-ground tissues of cadmium-treated tobacco plants were supposed to contain exclusively polyadenylated mRNAs. Surprisingly, numerous polyadenylated fragments matching parts of 26S rRNA were identified and their presence was confirmed by Northern blot and cDNA amplification techniques. To our knowledge this is the first report on rRNA polyadenylation in plants.
EN
We compared action of Cd2+, Hg2+, and Cu2+ on isolated rat liver mitochondria in the absence of added Ca2+ and Pi. The heavy-metal ions produced dose-dependently: (1) enhanced membrane permeabilizaton manifested in mitochondrial swelling and activation of basal respiration, (2) inhibition of uncoupler-stimulated respiration, and (3) membrane potential dissipation. Among the metals, Cu2+ exhibited maximal stimulatory effect on basal respiration and minimal inhibitory action on DNP-uncoupled respiration whilst Cd2+ promoted the strongest depression of uncoupled respiration and the largest swelling in NH4NO3 medium. Dithiothreitol induced a basal respiration release if added after high [Cd2+] and [Hg2+], and the stimulation was CsA-insensitive.
EN
We examined changes in profiles of isoflavonoids in roots of lupine (Lupinus luteus L. cv. Juno) seedlings in response to treatment with two heavy metals: cadmium (at 10 mg/l) and lead (at 150 mg/l). Overall, 21 flavonoid conjugates were identified in root extracts, some of them with up to six positional isomers. The total amount of all isoflavonoids increased by about 15 % in cadmium-treated plants and by 46 % in lead-treated ones. Heavy metals markedly increased the content of two compounds: 2'-hydroxygenistein glucoside and 2'-hydroxygenistein 7-O-glucoside malonylated. Possible functions of the identified isoflavonoids in yellow lupine exposed to heavy metal stress are discussed.
EN
Metallothionein (MT) was first discovered over 50 years ago, as a cadmiumbinding protein in the kidney of a horse. Metallothioneins are a class of ubiquitously occurring cysteine and metal-rich proteins of low molecular weight, containing sulfur-based metal clusters formed with zinc (Zn), cadmium (Cd), and copper (Cu). In mammals, four distinct MT isoforms designated MT-1 – MT-4 are identified. The major physiological functions of metallothioneins include the homeostasis of essential metals Zn and Cu and the protection against oxidative stress and toxic effects of heavy metals such as Cd. Cadmium ,which has been classified as class I carcinogen, can stimulate cell proliferation, inhibit DNA repair, impair apoptosis and promote the development of cancer in a number of tissues. Significant quantities of Cd are introduced into the environment both by natural and anthropogenic activities, with anthropogenic activities contributing 3–10 times more Cd to the environment than natural activities. Cadmium accumulates in the human body with a long biological half-life ranging from 10 to 30 years. Cd accumulation and the aberrant expression of MTs have been found to be associated with the development of many types of cancers, including breast cancer.
PL
Metalotioneina (MT) została po raz pierwszy odkryta ponad 50 lat temu, jako białko wiążące kadm w nerce konia. Metalotioneiny są grupą powszechnie występujących białek o niskiej masie cząsteczkowej, wysokiej zawartości reszt cysteiny, których grupy tiolowe wiążą jony takich metali jak kadm (Cd), cynk (Zn) i miedź (Cu). U ssaków wyróżnia się cztery izoformy metalotioneiny oznaczone jak MT-1 – MT-4. Do podstawowych funkcji metalotionein zalicza się utrzymanie homeostazy metali ciężkich, ochronę przed ich toksycznym działaniem, a także ochronę przed stresem oksydacyjnym. Kadm, który został zakwalifikowany do substancji karcynogennych I klasy, może między innymi stymulować proliferację komórek, hamować naprawę uszkodzeń DNA, zaburzać proces apoptozy oraz promować rozwój nowotworów w licznych tkankach. Kadm znajdujący się w środowisku naturalnym pochodzi ze źródeł naturalnych, jak i antropogenicznych, przy czym ilość kadmu dostająca się do środowiska w wyniku działalności człowieka od 3 do 10 razy przekracza ilości kadmu pochodzące ze źródeł naturalnych. Kadm akumuluje się w organizmie człowieka, a jego okres biologicznego półtrwania wynosi od 10 do 30 lat. Zarówno akumulacja Cd, jak i zaburzenia ekspresji metalotionein mogą mieć związek z rozwojem wielu typów nowotworów, w tym raka piersi.
EN
Dispersive liquid-liquid microextraction (DLLME) was applied to the separation and preconcentration of Cd(II) and Co(II) from water and food samples. The influence of the following analytical parameters on the quantitative recoveries of cadmium and cobalt were investigated: pH, extraction solvent volume, dispersing solvent volume and type, and concentration of chelating agent. Under the optimized conditions, the detection limits were 2 μg L-1 and 13 μg L-1 for Cd(II) and Co(II), respectively. The proposed method was validated by the analysis of certified samples and has been applied to the determination of these ions in environmental water samples, with recoveries of 98 – 101% for Co(II) and 94 – 120% for Cd(II). In food samples the recoveries fell between 100 – 110% and 101-120% for Co(II) and Cd(II), respectively.
EN
Twelve soil samples differing in physicochemical properties and total element contents were extracted by three sequential extraction procedures to determine As, Cd, Pb, and Zn bound to individual soil fractions and are defined by individual operational procedures. In the case of arsenic, two additional sequential extraction schemes were designed entirely for fractionation of soil containing arsenic were tested. The results confirmed that determination of element proportions bound to individual soil fractions is strongly dependent on the extracting agent and/or procedure applied within individual extracting schemes. As expected, absolute values of the elements released among the individual extracting procedures are weakly comparable. More reliable results were determined for the more mobile soil elements i.e. cadmium and zinc, in the fractions characterizing the most mobile proportions of investigated elements where significant correlations with basic soil characteristics were observed. In contrast, ambiguous results were observed for As and Pb, for both the individual extraction procedures and the effect of the soil characteristics. Regardless of the studied element, the poorest results were determined for reducible and oxidizable soil fractions. The application of at least two independent procedures or modification of the extraction scheme according to element investigated and/or particular soil characteristics can also be helpful in definition of element pattern in soils in further research.
EN
Previously, a stable cell suspension of cucumber tolerant to 100 µM CdCl2 was obtained (Gzyl & Gwóźdź, 2005, Plant Cell Tissue Organ Cult 80: 59-67). In this study, the relationship between the activity of antioxidant enzymes and cadmium tolerance of cucumber cells was analyzed. A cadmium-sensitive and the cadmium-tolerant cell lines were exposed to 100 µM and 200 µM CdCl2 and the activities of superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APOX) and guaiacol peroxidase (POX) were determined. In the sensitive cell line, a decrease of total activity of SOD and POX was observed, whereas the activity of CAT and APOX significantly increased in metal-supplemented medium. By contrast, in the tolerant cells, the total activity of antioxidant enzymes decreased (SOD, CAT) or was maintained at approximately the same level (APOX, POX). Moreover, a different pattern of isoenzyme activity was observed in the tolerant and sensitive cells. These results suggest that an enhanced activity of antioxidant enzymes is not directly involved in the increased tolerance to cadmium of the selected cucumber cell line.
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EN
Currently, much attention is paid to the search for the etiology of many eye diseases. It has been shown that environmental pollution with heavy metals (cadmium, lead, mercury) is of significant importance. One of these diseases is cataract, i.e. clouding of the lens of the eye. It turns out that heavy metals are deposited in the tissues of the eyes, damaging them and accelerating the disease. This work is aimed at presenting the problem of the influence of heavy metals on cataracts. The study compares the concentration of heavy metals between the control group (81 healthy volunteers) and the group of cataract patients (72 sick). Plasma was used in the research and the ICP-MS method was used.
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