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EN
Separation of ethanol from solutions with different concentrations in a BIOTRON bioreactor integrated with the direct contact membrane distillation system has been investigated. The experiments were carried out using immersed, capillary polypropylene membranes. The permeability of the membrane at the feed inlet temperatures of 308, 318 and 333K was determined. The permeate and ethanol flux was strongly affected by the vapour pressure which increased with the feed temperature and it was also associated with ethanol concentration in the feed. It was found that the membrane distillation can be successfully applied for the separation of volatile components such as ethanol.
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EN
Two-stage biogas production plant consists of two reactors: a hydrolyser and a fermentor. The bioreactor construction has to meet three requirements: low cost and simplicity of construction and good biomass mixing conditions with an application of appropriate method. This paper reports CFD modelling of hydraulic mixing in the tank to be applied in a two-stage industrial installation.
EN
A mathematical model for a two-phase fluidised bed bioreactor with liquid recirculation and an external aerator was proposed. A stationary nonlinear analysis of such a bioreactor for an aerobic process with double-substrate kinetics was carried out. The influences of a volumetric fraction of solid carriers in the liquid phase, the rate of active biomass transfer from the biofilm to the liquid, the concentration of carbonaceous substrate, the mean residence time of the liquid and the efficiency of the external aerator on the steady state characteristics of the bioreactor were described. A method for determination of the minimal recirculation ratio related to oxygen demand and fluidised bed conditions was presented. On the basis of the obtained results, it is possible to choose reasonable operating conditions of such plants and to determine constraints, while considering acceptable concentrations of a toxic substrate being degraded.
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issue 2
185-190
EN
Flexirubins are the unique type of bacterial pigments produced by the bacteria from the genus Chryseobacterium, which are used in the treatment of chronic skin disease, eczema etc. and may serve as a chemotaxonomic marker. Chryseobacterium artocarpi CECT 8497, an yellowish-orange pigment producing strain was investigated for maximum production of pigment by optimizing medium composition employing response surface methodology (RSM). Culture conditions affecting pigment production were optimized statistically in shake flask experiments. Lactose, l-tryptophan and KH2PO4 were the most significant variables affecting pigment production. Box Behnken design (BBD) and RSM analysis were adopted to investigate the interactions between variables and determine the optimal values for maximum pigment production. Evaluation of the experimental results signified that the optimum conditions for maximum production of pigment (521.64 mg/L) in 50 L bioreactor were lactose 11.25 g/L, l-tryptophan 6 g/L and KH2PO4 650 ppm. Production under optimized conditions increased to 7.23 fold comparing to its production prior to optimization. Results of this study showed that statistical optimization of medium composition and their interaction effects enable short listing of the significant factors influencing maximum pigment production from Chryseobacterium artocarpi CECT 8497. In addition, this is the first report optimizing the process parameters for flexirubin type pigment production from Chryseobacterium artocarpi CECT 8497.
EN
The paper presents the dynamic characteristics of a continuous tank bioreactor for microbiological process, with a developed predator-prey food chain. The presence of the predator microorganism considerably influences the position and stability character of steady-states. There appears to exist a wide range of unstable steady-states and high-amplitude oscillations of state variables. Without automatic control, the system can operate only in unsteady conditions. From technological point of view, this circumstance is unfavorable. It was shown that oscillations can be removed by employing automatic control with continuous P or PI controllers. Moreover, the use of a controller with integrating element causes removal of the predator from the bioreactor. The paper discusses an application of this phenomenon for practical purposes.
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