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EN
We present here the results of an exploration of the bacteriophage content of dairy wheys collected from milk plants localized in various regions of Poland. Thirty-three whey samples from 17 regions were analyzed and found to contain phages active against L. lactis strains. High phage titer in all whey samples suggested phage-induced lysis to be the main cause of fermentation failures. In total, over 220 isolated phages were examined for their restriction patterns, genome sizes, genetic groups of DNA homology, and host ranges. Based on DNA digestions the identified phages were classified into 34 distinct DNA restriction groups. Phage genome sizes were estimated at 14-35 kb. Multiplex PCR analysis established that the studied phages belong to two out of the three main lactococcal phage types - c2 and 936, while P335-type phages were not detected. Yet, analyses of bacterial starter strains revealed that the majority of them are lysogenic and carry prophages of P335-type in their chromosome. Phage geographical distribution and host range are additionally discussed.
EN
Intra-population variation of 18 cultivated carrot (Daucus carota L. ssp. sativus) populations of diverse origins was evaluated using codominant microsatellite (SSR) markers. Using 27 genomic and EST-derived SSR markers, 253 alleles were identified with a mean 9.4 alleles per marker. Most of the alleles (60.5%) were rare i.e., with the frequency ≤ 0.05 while only 3.95% of alleles occurred with frequency > 0.6. EST-derived SSR markers were less polymorphic than genomic SSR markers. Differences in allele occurrence allowed 16 out of 18 populations to be assigned to either the Western or Asian carrot gene pools with high probability. Populations could be also discriminated due to the presence of private alleles (25.3% of all alleles). Most populations had excess of alleles in the homozygous state indicating their inbreeding, although heterozygous loci were common in F1 hybrids. Genetic diversity was due to allelic variation among plants within populations (62% of total variation) and between populations (38%). Accessions originating from continental Asia and Europe had more allelic variants and higher diversity than those from Japan and USA. Also, allelic richness and variability in landraces was higher than in F1 hybrids and open-pollinated cultivars.
EN
The article presents the possible methods for determining biological or statistically significant differences between taxocenoses compared with respect to biodiversity. To obtain a complete description of biological differences between the compared hypothetical communities, the following indices were calculated: S (taxon richness), H (the Shannon index), Hmax (the maximum value of the Shannon index for the richness of taxa represented by the same number of individuals), Vd (a percentage value of covering the structural capacity of community, “evenness deficiency”), E (the MacArthur index - a taxon number (S) in a community for which the observed value of H equals Hmax), and Ps (a taxon richness shortage in percents). Moreover, a graphic profile method (Дд, Tj, and Lj profiles) was used for comparing the diversity of the communities. To obtain information about statistically significant differences in biodiversity between the analysed communities, rarefaction curves were applied. The curves are based on the null models and the Monte Carlo method. The rarefaction method resulted in determination of the statistical significance of the differences between taxon richness and Shannon's index values for the compared communities. The Vd and Ps indices and the profile method allowed concluding about the significance of the biological differences between taxocenoses, even when their values of Shannon's H indices were numerically similar.
EN
This study under the title of “the environmental challenges of declining mangroves: a study based on Puttalam District in Sri Lanka” was undertaken to attain the primary objective ‘to identify the factors for the declining of the mangroves in the study area’ and the secondary objectives ‘identify the environmental challenges due to the mangroves declining in the study area and to suggest was to control the decline and to provide awareness of this problem among the inhabitants’. Both, primary and secondary data were collected for this study. As primary data, questionnaire survey, direct observation and constructed discussion were used. As secondary data, statistic reports, previous researches, books and magazines were employed. According to the analysis, major environmental challenges were encountered. These included ‘land fragmentation, biodiversity destruction, decreasing endemism in mangrove, loss of herbs and coastal soil erosion’. To overcome these challenges, many recommendations were put forward.
EN
A survey on the cyanobacterial biodiversity of 7 mangrove environments viz. Vallikunnu, Kadalundi, Kallayi, Mangalavanam, Kumbalam, Kumarakom and Mekkara mangroves of Kerala was undertaken during 2014-2016. In total, 31 species of cyanobacteria coming under 10 genera and 4 families were recorded in all mangrove environments. Maximum species (13 species) of cyanobacteria were recorded from Kadalundi, whereas the minimum was recorded from Mangalavanam (2 species). The genus Oscillatoria was observed with maximum distribution (13 species), followed by Nostoc (5 species) and Lyngbya (3 species). The genus Aphanocapsa, Microcoleus and Scytonema (2 species each) and Chroococcus, Aphanothece, Merismopedia, and Phormidium (1 species each) shows equal distribution. As many as three species, namely Oscillatoria salina, Oscillatoria ornata and Oscillatoria vizagapatensis were common in all mangrove environments except Mangalavanam and Mekkara.
EN
Conserving biodiversity is a major necessity, as human interference causes loss of this. Tourism is not exempt from this. Still, ecotourism, a sub-component of the field of sustainable tourism, has more positive effects. The primary objective of this work is to ascertain the condition of ecotourism in the study area. The secondary objectives are to identify the challenges and progress of biodiversity by way of ecotourist activity, and to suggest solutions to promote best outcome and to overcome the challenges of ecotourism. China Bay (Marble Beach), Kanniya Hot Wells, Nilaveli Beach, Pullmottai Beach (Arisimalai), Lanka Patina and Trincomalee Beach are renown places for tourism in Trincomalee District. The condition of the ecotourism industry in Trincomalee District was identified by means of questionnaire survey and direct observation. Our work shows that the coastal areas are particularly rich in biodiversity and in offering scenic values. Many environmental challenges were identified and remedial measures, suggested.
EN
Aromatic plant species present in the natural Park of Tuscany Archipelago are used as flavoring agents and spices, as dietary supplements and in cosmetics and aromatherapy. The plants are usually collected from wild stands, inducing a depletion of the natural habitat. Therefore, micropropagation of these aromatic plants can play a role in the protection of the natural ecosystem, can guarantee a massive sustainable production and can provide standardized plant materials for diverse economical purposes. The aim of this study is to compare the volatile organic compounds produced by the wild plants with those from in vitro plantlets using headspace solid phase micro-extraction (HS-SPME) followed by capillary gas-chromatography coupled to mass spectrometry (GC-MS). Typical plants of this natural area selected for this work were Calamintha nepeta L., Crithmum maritimum L., Lavandula angustifolia L., Myrtus communis L., Rosmarinus officinalis L., Salvia officinalis L. and Satureja hortensis L. Different explants were used: microcuttings with vegetative apical parts, axillary buds and internodes. Sterilization percentage, multiplication rate and shoot length, as well as root formation were measured. The volatile aromatic profiles produced from in vitro plantlets were compared with those of the wild plants, in particular for C. maritimum, R. officinalis, S. officinalis and S. hortensis. This study indicated that the micropropagation technique can represent a valid alternative to produce massive and sterile plant material characterised by the same aromatic flavour as in the wild grown plants.
EN
The prime objective of this study is to assess the socio-economic impact of wetlands in the study area; the secondary objective is to establish the condition of the wetlands and to propose remedial measures. Primary and secondary data were used for this study. As primary data, we employed direct observation, questionnaire survey and group discussion. As secondary data, we utilized statistical data from Navithanveli DS Division, previous researches and general ecological works. In Navithenveli DS Division, inhabitants are benefitted by wetlands in many ways. Paddy cultivations, vegetable cultivation, small-scale fishing, biodiversity, groundwater recharge, gardening, Tule mat industry and water for domestic usages. Indiscriminate activities of inhabitants, unplanned and improper infrastructure have caused many challenges for the wetlands in the study area. Many remedial measures have been recommended to conserve the wetlands in the study area.
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