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Biodiversity of Lactococcus lactis bacteriophages in Polish dairy environment

100%

Szczepańska A. K., Hejnowicz M. S., Kołakowski P., Bardowski J.

Acta Biochimica Polonica

2007

vol. 54

issue 1

151-158

We present here the results of an exploration of the bacteriophage content of dairy wheys collected from milk plants localized in various regions of Poland. Thirty-three whey samples from 17 regions were analyzed and found to contain phages active against L. lactis strains. High phage titer in all whey samples suggested phage-induced lysis to be the main cause of fermentation failures. In total, over 220 isolated phages were examined for their restriction patterns, genome sizes, genetic groups of DNA homology, and host ranges. Based on DNA digestions the identified phages were classified into 34 distinct DNA restriction groups. Phage genome sizes were estimated at 14-35 kb. Multiplex PCR analysis established that the studied phages belong to two out of the three main lactococcal phage types - c2 and 936, while P335-type phages were not detected. Yet, analyses of bacterial starter strains revealed that the majority of them are lysogenic and carry prophages of P335-type in their chromosome. Phage geographical distribution and host range are additionally discussed.

A randomized, double-blind, placebo-controlled study to investigate the use of bacteriophages in patients with chronic rhinosinusitis with nasal polyps

88%

Dobretsov K. G., Kolenchukova O., Sipkin A., Bellussi L. M., Ciprandi G., Passali D.

Polish Journal of Otolaryngology

2021

vol. 75

issue 6

33-37

Introduction: A randomized, double-blind, placebo-controlled study investigates the use of bacteriophages in the treatment of chronic rhinosinusitis with nasal polyps. Material and methods: 40 adult patients with chronic rhinosinusitis with nasal polyps were examined. All patients underwent functional endoscopic sinus surgery. After the surgery, 20 patients were administered an intranasal gel with bacteriophage mixture (Otofag, Micromir, Russia) twice a day for ten weeks, while 20 other patients received a placebo solution. Results: On the 10th day, IL-1β secretion diminished (63 mg/ml versus 440 mg/ml in control). There was a decrease in the total number of microorganisms and Enterobacteriaceae (5.7 x 106 CFU/ml versus 1.2 x 109 CFU/ml in the control group) and the absence of Streptococci (versus 2.1 x 109 CFU/ml in control) was noted on the 30th day of treatment in the group receiving bacteriophage mixture. On the 10th day, a decrease in the activity of secretory IL-1β and IL-8 strongly and very strongly correlated with a total number of microorganisms (r = 0.7; r = 0.9, respectively), as well as a decrease in the activity of secretory IL-8 correlated with the number of Enterobacteriaceae (r = 0.72) and Staphylococci (r = 0.65) in the experimental group treated with bacteriophages. On the 30th day, the decrease in serum IL-1β significantly correlated with the total number of microorganisms (r = 0.80) and Enterobacteriaceae (r = 0.90) in the experimental group. Conclusions: The administration of bacteriophages restored the balance of microorganisms in the nasal cavity and decreased inflammatory response in chronic rhinosinusitis with nasal polyps. Changes such as an inflammation dampening could potentially contribute to reducing recurrent growth of polyp tissue in the future.

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1 Acta Biochimica Polonica

1 Polish Journal of Otolaryngology

1 2021

1 2007

1 Bardowski J.

1 Bellussi L. M.

1 Ciprandi G.

1 Dobretsov K. G.

1 Hejnowicz M. S.

1 Kolenchukova O.

1 Kołakowski P.

1 Passali D.

1 Sipkin A.

1 Szczepańska A. K.

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Biodiversity of Lactococcus lactis bacteriophages in Polish dairy environment

A randomized, double-blind, placebo-controlled study to investigate the use of bacteriophages in patients with chronic rhinosinusitis with nasal polyps