Search results

1

Evaluation of the enzymatic activity of selected bacterial strains

100%

Wierzba S., Latała A.

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EN

In these studies we attempted to evaluate the lipolytic, proteolytic and cellulolytic activity of bacterial strains isolated from water and the bottom sediments of Turawa Lake. The following bacterial genera prevailed among the isolated strains: Bacillus, Pseudomonas, Enterobacter, Cellulomonas and Cytophaga. The lipolytic activity was determined using a titrimetric method, the proteolytic activity - using a modified Anson method, and the cellulolytic activity - on the basis of mass decrement of a cellulose disk after 14 days of bacterial culture. The cultures were maintained at 28°C, pH 7.0 with the following substrates: olive oil, albumin and cellulose disk. Among the analysed microorganisms, Bacillus and Pseudomonas strains showed the highest lipolytic and proteolytic activity. In the cellulolytic assay Cytophaga bacteria showed about twofold higher activity than that of Celulomonas.

2

Abundance and biomass of bacteria in two Arctic glacial fjords

88%

Jankowska K., Włodarska-Kowalczuk M., Wieczorek P., Jankowska K., Włodarska-Kowalczuk M., Wieczorek P.

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2005

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vol. 26

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issue 1

3

Identification and properties of the Deinococcus grandis and Deinococcus proteolyticus single-stranded DNA binding proteins (SSB)

88%

Filipkowski P., Kur J.

Acta Biochimica Polonica

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2007

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vol. 54

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issue 1

79-87

EN

To study the biochemical properties of SSB's from Deinococcus grandis (DgrSSB) and Deinococcus proteolyticus (DprSSB), we have cloned the ssb genes obtained by PCR and have developed Escherichia coli overexpression systems. The genes consist of an open reading frame of 891 (DgrSSB) and 876 (DprSSB) nucleotides encoding proteins of 296 and 291 amino acids with a calculated molecular mass of 32.29 and 31.33 kDa, respectively. The amino-acid sequence of DgrSSB exhibits 45%, 44% and 82% identity and the amino-acid sequence of DprSSB reveals 43%, 43% and 69% identity with Thermus aquaticus (TaqSSB), Thermus thermophilus (TthSSB) and Deinococcus radiodurans SSBs, respectively. We show that DgrSSB and DprSSB are similar to Thermus/Deinococcus SSBs in their biochemical properties. They are functional as homodimers, with each monomer encoding two single-stranded DNA binding domains (OB-folds). In fluorescence titrations with poly(dT), both proteins bind single-stranded DNA with a binding site size of about 33 nt per homodimer. In a complementation assay in E. coli, DgrSSB and DprSSB took over the in vivo function of EcoSSB. Thermostability with half-lives of about 1 min at 65-67.5°C make DgrSSB and DprSSB similar to the known SSB of Deinococcus radiodurans (DraSSB).

4

Cholelithiasis – always infected?

88%

Matyjas T., Pomorski L., Witas H., Płoszaj T., Matyjas K., Kaczka K.

Polish Journal of Surgery

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2017

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vol. 89(3)

23-26

EN

This study aims to present results regarding the presence and identification of bacterial strains found in bile and gallstones located in the gallbladder and bile ducts in patients operated on due to cholelithiasis. Materials and Methods. Bacterial culture was evaluated in 92 patients. There were 54 women (59%) and 38 men (41%) who underwent surgery on account of cholelithiasis and /or gallstones in bile ducts between 2013 and 2014. Bile and gallstone samples were cultured intraoperatively for bacteria; bacterial strains were identified, and their sensitivity to antibiotics was determined. Molecular methods (NGS and Sanger method) were used to separate bacterial strains in one of the gallbladder stones and the results were compared with bacterial strains grown from the bile. Results. Bile cultures were positive in 46 patients that is, 50% of the study group. The following bacteria strains were grown: Enterococcus spp. (44%), Escherichia coli (37%) and Klebsiella spp. (35%). Candidiasis accompanied by bacterial infection was detected in 7 patients (15%). Molecular testing of gallstones revealed DNA of Enterococcus spp., Escherichia spp., Streptococcus spp. and Clostridium spp. In the bile culture of the same patient Enterococcus spp. (avium and faecalis) was detected. Conclusion 1. More than one pathogen was grown on samples obtained from 31 patients (70%) with bile infection. 2. The most common pathogens include Enterococcus spp., Escherichia coli and Klebsiella spp. 3. Bacterial infections are often accompanied by a fungal infection (Candida albicans) 4. Bacterial strains grown from a gallstone sample partially corresponded with strains identified in the bile of the same patient.

5

A randomized, double-blind, placebo-controlled study to investigate the use of bacteriophages in patients with chronic rhinosinusitis with nasal polyps

88%

Dobretsov K. G., Kolenchukova O., Sipkin A., Bellussi L. M., Ciprandi G., Passali D.

Polish Journal of Otolaryngology

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2021

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vol. 75

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issue 6

33-37

EN

Introduction: A randomized, double-blind, placebo-controlled study investigates the use of bacteriophages in the treatment of chronic rhinosinusitis with nasal polyps. Material and methods: 40 adult patients with chronic rhinosinusitis with nasal polyps were examined. All patients underwent functional endoscopic sinus surgery. After the surgery, 20 patients were administered an intranasal gel with bacteriophage mixture (Otofag, Micromir, Russia) twice a day for ten weeks, while 20 other patients received a placebo solution. Results: On the 10th day, IL-1β secretion diminished (63 mg/ml versus 440 mg/ml in control). There was a decrease in the total number of microorganisms and Enterobacteriaceae (5.7 x 106 CFU/ml versus 1.2 x 109 CFU/ml in the control group) and the absence of Streptococci (versus 2.1 x 109 CFU/ml in control) was noted on the 30th day of treatment in the group receiving bacteriophage mixture. On the 10th day, a decrease in the activity of secretory IL-1β and IL-8 strongly and very strongly correlated with a total number of microorganisms (r = 0.7; r = 0.9, respectively), as well as a decrease in the activity of secretory IL-8 correlated with the number of Enterobacteriaceae (r = 0.72) and Staphylococci (r = 0.65) in the experimental group treated with bacteriophages. On the 30th day, the decrease in serum IL-1β significantly correlated with the total number of microorganisms (r = 0.80) and Enterobacteriaceae (r = 0.90) in the experimental group. Conclusions: The administration of bacteriophages restored the balance of microorganisms in the nasal cavity and decreased inflammatory response in chronic rhinosinusitis with nasal polyps. Changes such as an inflammation dampening could potentially contribute to reducing recurrent growth of polyp tissue in the future.

6

The impact of composts prepared from different organic waste on the total number of soil microorganisms

88%

Michalcewicz W., Wołoszyk C., Balcer K.

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EN

In a two-factor pot experiment impact of two doses of six types of composts prepared from the municipal sewage sludge with an addition of canteen waste, wheat straw and cocoa husk on the total number of bacteria, actinomycetes and fungi in the soil were exmined. The light soil used in the experiment was taken from the Ap level of an arable field. On all the objects with composts there was mineral NPK fertilization applied, the control object was fertilized with the NPK only. The test plant was grass Festulolium which was harvested three times and after the third cut the samples of the soil were taken for microbiological analyses.The conducted research shows that the number of microorganisms in soil was dependent on the dose and the type of the compost. The second dose of the compost, in comparison with the control object, in most composts considerably increased the number of bacteria and the first dose increased the number of actinomycetes. However, the number of fungi, in most cases, with both doses of composts, was lower than in the soil from the control object. The highest number of bacteria was noticed on the object with the co-most prepared from 35% sewage sludge + 35% canteen waste + 30% straw and actinomycetes on the object with the compost prepared from 35% sewage sludge + 35% canteen waste + 30% cocoa husk. The development of the soil fungi was restrained in the highest degree by the first dose of the compost prepared from 35% sewage sludge + 35% canteen waste + 15% straw + 15% cocoa husk and by the second dose of the compost prepared from 70% sewage sludge + 30% straw.

7

MID and UspA1/A2 of the human respiratory pathogen Moraxella catarrhalis, and interactions with the human host as basis for vaccine development

88%

Riesbeck K., Tan T. T., Forsgren A.

Acta Biochimica Polonica

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2006

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vol. 53

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issue 3

445-456

EN

Moraxella catarrhalis IgD-binding protein MID is a 200 kDa autotransporter protein that exists as a oligomer and is governed at the transcriptional level. The majority of M. catarrhalis clinical isolates expresses MID. Two functional domains have been attributed to MID; MID764-913 functions as an adhesin and promotes the bacteria to attach to epithelial cells, whereas the IgD-binding domain is located within MID962-1200. In parallel, MID is stimulatory for B lymphocytes through the IgD B cell receptor. M. catarrhalis ubiquitous surface proteins A1 and A2 (UspA1/A2) are multifunctional outer membrane proteins that can bind complement and extracellular matrix proteins such as vitronectin and fibronectin. An interaction between the complement fluid phase regulator of the classical pathway, C4b binding protein (C4BP), and UspA1/A2 has also been observed. Moreover, UspA1/A2 has a unique feature to interfere with the innate immune system of complement by binding C3. Taken together, a growing body of knowledge on M. catarrhalis outer membrane proteins MID and UspA1/A2 and their precise interactions with the human host make them promising vaccine candidates in a future multicomponent vaccin.

8

Bacteria in pack-ice north of Elephant Island (BIOMASS III, October 1986)

88%

Zdanowski M. K., Zdanowski M. K.

Polish Polar Research

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1988

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vol. 9

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issue 2-3

9

Assessment of microbiological quality of air in the selected sites situated by the main roads of Kraków

75%

Lenart-Boroń A., Juraszek K.

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vol. 17

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issue 2

15-22

EN

Introduction. Kraków is one of the most beautiful but also one of the most crowded Polish cities with large numbers of cars, pedestrians and cyclists travelling each day. There has been an increasing concern about the human exposure to bioaerosols, which can occur, among others, at the sites characterized by increased dustiness, such crowded streets. The aim of this study was to evaluate the microbiological quality of air in the selected sites situated by the main roads in Kraków. Material and methods. Air samples were collected in 10 sites located by the main roads, using a MAS-100 impactor four times per year. Four microbial groups were enumerated: mesophilic bacteria, fungi, actinomycetes and staphylococci. The results were expressed as colony forming units per m3 of air and compared with Polish Standards concerning microbiological air quality. Results. The greatest bacterial and fungal aerosol concentration was observed in autumn, when atmospheric conditions could have promoted abundance of those microorganisms. In general, fungi were the most numerous group of airborne microorganisms, while staphylococci were the least numerous. The number of actinomycetes was alarmingly high in all sites. It was found that seasonal differences in the bioaerosol concentration were statistically significant and the recorded differences could have been affected by atmospheric conditions. Conclusions. The conducted studies showed that in terms of airborne bacteria and fungi, the air in the examined locations was microbiologically unpolluted. However, high numbers of airborne actinomycetes were observed in all sites, which can cause adverse health effects in pedestrians or cyclists who are frequently exposed to bioaerosols by the main routes in Kraków. Statistically significant variability was found in the prevalence of the examined microorganisms in different seasons of the year.

PL

Wstęp. Kraków jest jednym z najpiękniejszych i zarazem jednym z najbardziej zatłoczonych polskich miast, w którym każdego dnia po ulicach przemieszczają się tysiące samochodów, a także przechodniów i rowerzystów. Coraz większe zaniepokojenie budzi problem narażenia ludzi na aerozol biologiczny, którego podwyższone stężenie może wystąpić między innymi w zapylonych miejscach, takich jak zatłoczone ulice. Z tego powodu, celem badań była ocena jakości mikrobiologicznej powietrza w wybranych punktach przy głównych szlakach komunikacyjnych Krakowa. Materiał i metody. Próbki powietrza pobierano w 10 punktach umiejscowionych wzdłuż głównych dróg, czterokrotnie w ciągu roku, przy użyciu impaktora MAS- 100. Oznaczono liczebność czterech grup mikroorganizmów – bakterii mezofilnych, grzybów, promieniowców i gronkowców. Wyniki wyrażono jako jednostki tworzące kolonie w m3 powietrza i porównano z Polskimi Normami dotyczącymi jakości mikrobiologicznej powietrza atmosferycznego. Wyniki. Najwyższe stężenie bioaerozolu bakteryjnego i grzybowego stwierdzono jesienią, gdy warunki atmosferyczne były korzystne dla zwiększonej liczebności drobnoustrojów w powietrzu. Ogółem, grzyby były najliczniejszą grupą drobnoustrojów, a najmniej liczną były gronkowce. We wszystkich badanych lokalizacjach liczebność promieniowców była niepokojąco wysoka. Stwierdzono istnienie istotnej statystycznie zmienności sezonowej w stężeniu bioaerozolu, przy czym różnice te mogły być wynikiem zmiennych warunków atmosferycznych. Wnioski. Przeprowadzone badania wykazały, że pod względem stężenia bakterii i grzybów powietrze w badanych lokalizacjach było mikrobiologicznie czyste. Jednakże we wszystkich lokalizacjach stwierdzano wysokie stężenie promieniowców, co może prowadzić do niekorzystnych skutków zdrowotnych np. u pieszych i rowerzystów, którzy często narażeni są na kontakt z bioaerozolem, podróżując wzdłuż głównych dróg w Krakowie. Stwierdzono istotną statystycznie zmienność liczebności badanych drobnoustrojów w różnych porach roku.

10

Biofouling reduction for improvement of depth water filtration. Filter production and testing

75%

Sztuk-Sikorska E., Leon G.

Chemical and Process Engineering

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2016

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vol. 37

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issue 3

11

Occurrence and Antibiotic Resistance Profile of Bacteria Isolated from Freshly Sliced Ready to Eat Fruits Sold Across Major Retail Outlets within Owerri Metropolis

75%

Dike K. S.

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EN

In recent years, there has been a substantial rise in the consumption of sliced ready-to-eat fruits sold across numerous retail outlets in Nigeria. This is because of their high accessibility, convenience, and, most significantly, their lower cost than whole fruits. Nevertheless, these food items have continually acted as carriers for human diseases on a global scale. This study evaluated the occurrence and antibiogram of bacteria from ready-to-eat sliced fruits sold across major retail outlets in the Owerri metropolis. One hundred and fifty samples comprising oranges, watermelons, pineapples, and paw-paws were analysed using standard microbiological techniques. Bacteria isolates were identified using cultural, biochemical, and molecular methods. Antibiotic sensitivity was performed using the Kirby-Bauer diffusion technique. Results showed that orange samples had the highest total viable count (2.50 log cfu/g), total coliform count (2.70 log cfu/g), and faecal coliform count (2.05 log cfu/g), respectively. Staphylococcus aureus predominated in the fruit samples with a percentage occurrence of 29.4%. Isolates exhibited different resistance levels, with Staphylococcus aureus and Bacillus spp showing a high resistance against Gentamycin and ofloxacin. These findings demand further investigation due to the possible health risks of coliform bacteria, faecal coliform and antibiotic resistance. Therefore, monitoring and implementing food safety standards is necessary to protect consumers.

12

Melanin synthesis in microorganisms - biotechnological and medical aspects

75%

Plonka P. M., Grabacka M.

Acta Biochimica Polonica

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2006

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vol. 53

|

issue 3

429-443

EN

Melanins form a diverse group of pigments synthesized in living organisms in the course of hydroxylation and polymerization of organic compounds. Melanin production is observed in all large taxa from both Pro- and Eukaryota. The basic functions of melanins are still a matter of controversy and speculation, even though their adaptative importance has been proved. Melanogenesis has probably evolved paralelly in various groups of free living organisms to provide protection from environmental stress conditions, but in pathogenic microorganisms it correlates with an increased virulence. The genes responsible for melanization are collected in some cases within operons which find a versatile application in genetic engineering. This review sumarizes current views on melanogenesis in Pro- and Eukaryotic microorganisms in terms of their biotechnological and biomedical importance.

13

Integrated optical biosensor for rapid detection of bacteria

75%

Mathesz A., Valkai S., Újvárosy A., Aekbote B., Sipos O., Stercz B., Kocsis B., Szabó D., Dér A.

Optofluidics, Microfluidics and Nanofluidics

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2015

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vol. 2

|

issue 1

EN

In medical diagnostics, rapid detection of pathogenic bacteria from body fluids is one of the basic issues. Most state-of-the-art methods require optical labeling, increasing the complexity, duration and cost of the analysis. Therefore, there is a strong need for developing selective sensory devices based on label-free techniques, in order to increase the speed, and reduce the cost of detection. In a recent paper, we have shown that an integrated optical Mach-Zehnder interferometer, a highly sensitive all-optical device made of a cheap photopolymer, can be used as a powerful lab-on-a-chip tool for specific, labelfree detection of proteins. By proper modifications of this technique, our interferometric biosensor was combined with a microfluidic system allowing the rapid and specific detection of bacteria from solutions, having the surface of the sensor functionalized by bacterium-specific antibodies. The experiments proved that the biosensor was able to detect Escherichia coli bacteria at concentrations of 106 cfu/ml within a few minutes, that makes our device an appropriate tool for fast, label-free detection of bacteria from body fluids such as urine or sputum. On the other hand, possible applications of the device may not be restricted to medical microbiology, since bacterial identification is an important task in microbial forensics, criminal investigations, bio-terrorism threats and in environmental studies, as well.

14

Bacteriological evaluation of fish pond waste water from different fish ponds in Owerri Imo State, Nigeria

75%

Chidinma M. M.

World News of Natural Sciences

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2024

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vol. 53

223-230

EN

The importance of fish farming can never be over emphasized because it’s a major provider of dietary protein, source of income and employment for the growing populations in Nigeria. Similarly, the importance of the bacteriological evaluation of fish pond water can never be over emphasized as it is a major indicator of the suitability and sanitary condition of the aquaculture pond and the fish in particular. This study evaluated the bacteriological profile of different fish pond water located within Owerri metropolis. A total of 10 fish pond waste water samples were collected and analyzed using different selective microbiological media and isolates identified using gram staining and biochemical reactions. Result shows that the total heterotrophic bacteria count ranged from 6.4×103 CFU/mL - 7.5×107 CFU/mL, total coliform count ranged from 2.1×104 CFU/mL - 5.6×107 CFU/mL, total vibrio count ranged from 2.7×103 CFU/mL - 5.9×105 CFU/mL while the Salmonella / Shigella count ranged from 1.9×105 CFU/mL - 5.9×105 CFU/mL. A total of nine bacterial isolates were identified which includes Staphylococcus spp, Escherichia spp, Vibrio spp, Salmonella spp, Enterobacter spp, Shiglla spp and Klebsiela spp. The result revealed a high bacterial load in the fish pond water and therefore recommends constant water change to prevent the possible buildup of potential pathogenic bacteria in fish and introduction of these organisms into the environment.

15

Distribution of bacteria, organic carbon and amino acids in the southern part of Drake Passage and in Bransfield Strait during the BIOMASS - SIBEX (December1983 —January 1984)

75%

Zdanowski M. K., Zdanowski M. K.

Polish Polar Research

|

1985

|

vol. 6

|

issue 1-2

16

Bioaerozole w budynkach biurowych

63%

Gołofit-Szymczak M., Górny R.

Kosmos

|

2017

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vol. 66

|

issue 3

491-502

PL

W związku z dynamicznym rozwojem budownictwa biurowego oraz związanym z nim znaczącym wzrostem liczby pracowników zatrudnionych w pomieszczeniach przeznaczonych do pracy biurowej, czystość powietrza tego typu wnętrz ma istotne znaczenie dla zdrowia i samopoczucia ludzi w nich pracujących. Budynki są stale narażone na kolonizację przez mikroorganizmy. Źródłem zanieczyszczeń mikrobiologicznych pomieszczeń są pracownicy, elementy konstrukcyjne i wyposażenie budynków, instalacje wentylacyjne (klimatyzacyjne) oraz powietrze zewnętrzne. W środowisku wnętrz, czynniki biologiczne (np. wirusy, bakterie, grzyby, endotoksyny, glukany lub mikotoksyny), będąc transportowane drogą powietrzną mogą powodować wiele niekorzystnych skutków zdrowotnych u narażonych osób.

EN

Due to the dynamic development of office building industry and subsequent significant increase of the number of employees working in premises dedicated to office work, the indoor air quality is of a great importance for both the human health and well-being. The buildings are constantly exposed to microbial colonisation. Among the major sources of microbial contamination of premises are employees, construction materials, ventilation (air-conditioning) systems and outdoor air. Biological agents (i.e. viruses, bacteria, fungi, endotoxins, glucans or mycotoxins) which are transported in the air in to the indoor environment, can cause numerous adverse health outcomes in exposed individuals.

17

Mechanizmy adaptacyjne umożliwiające życie bakterii w wysokich temperaturach

63%

Hus K., Bocian A.

Kosmos

|

2017

|

vol. 66

|

issue 2

175-184

PL

Z antropocentrycznego punktu widzenia, środowiska cechujące się wysokimi temperaturami opisywane są jako ekstremalne. Pierwotnie uważano, że są one zbyt niekorzystne dla rozwoju życia, jednakże wiele badań naukowych dowiodło, iż istnieje spora grupa mikroorganizmów, które mogą przetrwać w tak trudnych warunkach. Jednakże aby było to możliwe, organizmy te wykształciły wiele mechanizmów i strategii ochrony komórki przed niekorzystnymi warunkami środowiska. Zaliczyć tu można: produkcję białek szoku cieplnego, stabilizację struktury DNA, błyskawiczną resyntezę ATP, aminokwasów i innych termolabilnych składników komórki, syntezę trehalozy i innych cząsteczek stabilizujących struktury komórkowe, zwiększoną syntezę specyficznych proteaz, zastąpienie nukleotydów nikotynamidowych przez stabilniejszą ferredoksynę czy zmianę ekspresji genów w komórce. Enzymy produkowane przez mikroorganizmy termofilne są obecnie źródłem intensywnych badań, głównie ze względu na swoje wyjątkowe właściwości i szerokie zastosowanie w przemyśle.

EN

From the anthropocentric point of view, the environments that are characterized by high temperatures have been identified as extreme ones. Originally, they were considered as too extreme to allow any organism to survive. However, later investigations have revealed that there exists a fairly large group of microorganisms thriving very well in these conditions. In order to withstand high temperatures these microorganisms have developed numerous mechanisms and strategies for protecting their cells. They include inter alia production of heat shock proteins, stabilization of the double-stranded DNA structure, rapid re-synthesis of ATP, certain amino acids and other heat-labile components of the cell, enhanced synthesis of: trehalose and other molecules stabilizing cell structures, and specific proteases hydrolyzing denatured proteins, substitution of termo-labile nicotinamide adenine dinucleotides by more thermally stable ferredoxin, as well as modifications of gene expression. Presently, enzymes produced by thermophilic microorganisms are an important area of research owing to their unique properties and wide industrial applications.

18

Medyczne zastosowania laktoferyny

51%

Gajda-Morszewski P., Śpiewak K.

Zeszyty Naukowe Towarzystwa Doktorantów Uniwersytetu Jagiellońskiego. Nauki Ścisłe

|

2015

|

issue 11

67-75

EN

Lactoferrin is a secreted protein found in high concentrations in colostrum and milk, less aboundant in saliva, tears, semen and mucus etc. etc., has been attracting the attention of researchers because of the broad spectrum of action. Lactoferrin has an antibacterial, antifungal, antiviral, immunomodulatory, antineoplastic activity and many others. The following article describes the results of recent research on medical applications of lactoferrin. This protein is very interesting because of the variety of functions performed in the absence of toxicity, as evidenced by the certificates have GRAS (Generally Recognized as Safe).

PL

Laktoferyna, białko wydzielnicze występujące w dużym stężeniu w siarze i mleku, w mniejszym w ślinie, łzach, nasieniu, śluzie itd., od lat przyciąga uwagę badaczy ze względu na szerokie spektrum działania. Przypisuje się jej działanie antybakteryjne, przeciwgrzybicze, antywirusowe, immunomodulacyjne, przeciwnowotworowe oraz wiele innych. W poniższym artykule opisano wyniki najnowszych badań dotyczących medycznych zastosowań laktoferyny. Białko to jest niezwykle interesujące ze względu na różnorodność pełnionych funkcji przy jednoczesnym braku toksyczności, czego dowodem jest posiadanie certyfikatu GRAS (Generally Recognized as Safe).

19

Molecular Monitoring Of Bacterial And Microalgal Biocenoses’ Biodiversity In High Loaded Farming Ponds

51%

Karło A., Ziembińska-Buczyńska A., Pilarczyk M., Surmacz-Górska J.

|

issue 3

425-437

EN

Eutrophication process is a serious problem in water ecosystems. There is a great need to study the relation between the physico-chemical condition of water and the influence of these parameters on the diversity of biological life, especially on changes in the structure of microbiocenoses. The most interesting are bacteria and microalgae, due to the important roles they play in maintaining the balance of the aquatic environment. In this study, biodiversity analysis of eukaryotic microalgae and bacteria in two artificial water ecosystems - fish farming ponds - was performed. Aquaculture was based on IMTA technology, in which every part of the trophic chain plays a significant role in maintaining the balance in the ecosystems. Experimental intensive - extensive systems differed in terms of nutrient loads, ponds were characterized by high loads of organic and inorganic nitrogen and phosphorus. During the experimental period, the physicochemical conditions, quantitative genotypic structure of the two biocenoses being studied and the relation between these factors were monitored and investigated. For the biodiversity analysis, the PCR - DGGE technique was used. The results of preliminary research showed that there is a correlation between nutrient loads, diversity expressed in the Shannon-Wiener Index and the overall condition of experimental systems. Higher loadings of nutrient promote the development of bacteria and microalgae without any influence on the balance in the artificial ecosystem being tested.

Refine search results

Evaluation of the enzymatic activity of selected bacterial strains

Abundance and biomass of bacteria in two Arctic glacial fjords

Identification and properties of the Deinococcus grandis and Deinococcus proteolyticus single-stranded DNA binding proteins (SSB)

Cholelithiasis – always infected?

A randomized, double-blind, placebo-controlled study to investigate the use of bacteriophages in patients with chronic rhinosinusitis with nasal polyps

The impact of composts prepared from different organic waste on the total number of soil microorganisms

MID and UspA1/A2 of the human respiratory pathogen Moraxella catarrhalis, and interactions with the human host as basis for vaccine development

Bacteria in pack-ice north of Elephant Island (BIOMASS III, October 1986)

Assessment of microbiological quality of air in the selected sites situated by the main roads of Kraków

Biofouling reduction for improvement of depth water filtration. Filter production and testing

Occurrence and Antibiotic Resistance Profile of Bacteria Isolated from Freshly Sliced Ready to Eat Fruits Sold Across Major Retail Outlets within Owerri Metropolis

Melanin synthesis in microorganisms - biotechnological and medical aspects

Integrated optical biosensor for rapid detection of bacteria

Bacteriological evaluation of fish pond waste water from different fish ponds in Owerri Imo State, Nigeria

Distribution of bacteria, organic carbon and amino acids in the southern part of Drake Passage and in Bransfield Strait during the BIOMASS - SIBEX (December1983 —January 1984)

Bioaerozole w budynkach biurowych

Mechanizmy adaptacyjne umożliwiające życie bakterii w wysokich temperaturach

Medyczne zastosowania laktoferyny

Molecular Monitoring Of Bacterial And Microalgal Biocenoses’ Biodiversity In High Loaded Farming Ponds