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Efficacy of mistletoe for chemical pleurodesis in rats without malignancy

100%
Ahn H. Y., Cho J. S., Kim Y. D., I H., Kim Y. J., Kim A., Lee C. H.
Open Medicine
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2014
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vol. 10
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issue 1
EN
Chemical pleurodesis is an effective treatment modality to reduce recurrence of malignant effusion. Several agents have been used in chemical pleurodesis but, it is not yet clear which is better. Eighteen Sprague- Dawley rats were used and classified into three groups: a group intrapleurally injected normal saline (group A, n=6), 400mg/kg talc (group B, n=6), and 9mg/kg mistletoe extraction (ME) (group C, n=6). Autopsy was performed to evaluate the pleural adhesion, pathologic examination of pleura and lung and bronchoalveolar lavage fluid analysis 4 weeks after pleurodesis. Both group B and C showed an obvious pleural adhesion and there was no significant difference in grade of pleural adhesion between two groups (p=0.58). The parietal pleural thickness in talc group than ME group was significantly thicker (p=0.002) and the visceral pleura of talc group showed marked foreign body reaction with fibrosis and many multinucleated giant cells associated with talc crystal. This study suggests that pleurodesis using ME in condition without malignancy has comparable effect to pleurodesis using talc. However, additional experimental study in large animal or clinical trials would be required to prove a safety and an efficacy of pleurodesis using ME.
2
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The use of a one-step PCR method for the identification of Microsporum canis and Trichophyton mentagrophytes infection of pets

100%
Dąbrowska I., Dworecka-Kaszak B., Brillowska-Dąbrowska A.
Acta Biochimica Polonica
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2014
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vol. 61
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issue 2
375-378
EN
Introduction: Dermatophytes are a closely related group of keratinophilic fungi. They encompass important etiological agents of superficial fungal infections. These fungi are able to invade keratinized tissues of humans and animals, causing dermatophytosis (ringworm) of hair, nails or skin. The aim: Traditional diagnostics of ringworm is based on morphological identification of cultured fungi and is time-consuming. Materials and methods: In this study, we applied a method patented by Brillowska-Dabrowska and coworkers (Brillowska-Dąbrowska A, Saunte DM, Arenderup MC, 2007, Five-hour diagnosis of dermatophyte nail infections with specific detection of Trichophyton rubrum. J Clin Microbiol 45: 1200-1204) which involves extraction of fungal DNA and PCR amplification with pan-dermatophyte primers to confirm the presence of dermatophytes. Results: The method used here is able to confirm the presence of dermatophyte DNA in pure cultures in less than 5 hours.
3
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No cheek bias for non-primates: an instagram replication of thomas et al. (2006)

88%
Lindell A. K.
Acta Neuropsychologica
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2023
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vol. 21(3)
251-257
EN
Previous research has established that photos of great apes, including humans, show a left cheek bias. As this bias is absent in images of lower primates and other animals, phylo-genetic proximity appears to influence humans’ depictions of nonhuman species. However Thomas et al.’s (2006) finding of a left cheek bias for dogs challenges this argument. As their analyses were underpowered, the present study sought to replicate Thomas et al.’s study with a larger sample to help determine whether human depictions of non-human animals vary as a function of their evolutionary relatedness. Photographs (N=2883) were sourced from Instagram’s ‘Most Recent’ feed using hashtags that matched Thomas et al.’s Google Image search terms: #dog, #cat, #fish, #lizard, #cute- baby, #cryingbaby. The first 401 lateral images for each hashtag were coded for pose orientation (left, right). Replicating Thomas et al., results confirmed a left cheek bias for mammals but not nonmammals. The left cheek bias was driven by images of human infants; there were no cheek biases for images of nonhuman animals (dogs, cats, lizards, fish). As a left cheek bias was evident in photos of primates (#cutebaby, #cryingbaby), but absent for other mammals (#dog, #cat) and nonmammals (#lizard, #fish), the data support the argument that phylogenetic proximity influences posing biases.
4
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Host Range of Cyclospora Species: Zoonotic Implication

75%
Solarczyk P.
Acta Protozoologica
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2021
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vol. 60
13-20
EN
Cyclospora is an intracellular, gastrointestinal parasite found in birds and mammals worldwide. Limited accessibility of the protozoan for experimental use, scarcity, genome heterogeneity of the isolates and narrow panel of molecular markers hamper zoonotic investigations. One of the significant limitation in zoonotic studies is the lack of precise molecular tools that would be useful in linking animal vectors as a source of human infection. Strong and convincing evidence of zoonotic features will be achieved through proper typing of Cyclospora spp. taxonomic units (e.g. species or genotypes) in animal reservoirs. The most promising method that can be employ for zoonotic surveys is next-generation sequencing.
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