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1

Molecular markers in breeding for virus resistance in barley

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Ordon F., Friedt W., Scheurer K., Pellio B., Werner K., Neuhaus G., Huth W., Habekuss A., Graner A.
Journal of Applied Genetics
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2004
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vol. 45
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issue 2
145-159
EN
The soil-borne barley yellow mosaic virus disease (BaMMV, BaYMV, BaYMV-2) and the aphid-transmitted barley yellow dwarf virus (BYDV) are serious threats to winter barley cultivation. Resistance to barley yellow mosaic virus disease has been identified in extensive screening programmes and several recessive resistance genes have been mapped, e.g. rym4, rym5, rym9, rym11, rym13. In contrast to barley yellow mosaic virus disease, no complete resistance to BYDV is known in the barley gene pool, but tolerant accessions have been identified and QTL for BYDV-tolerance have been detected on chromosomes 2HL and 3HL. The use of resistance and tolerance in barley breeding can be considerably improved today by molecular markers (RFLPs, RAPDs, AFLPs, SSRs, STSs, SNPs), as they facilitate (i) efficient genotyping and estimation of genetic diversity; (ii) reliable selection on a single plant level independent of symptom expression in the field (iii) acceleration of back crossing procedures; (iv) pyramiding of resistance genes; (v) detection of QTL and marker-based combination of positive alleles; and (vi) isolation of resistance genes via map-based cloning.
2

A new diagnostic SSR marker for selection of the Rym4/Rym5 locus in barley breeding

100%
Tyrka M., Perovic D., Wardynska A., Ordon F.
Journal of Applied Genetics
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2008
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vol. 49
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issue 2
127-134
EN
Genomic sequence AY661558, representing a part of the BAC contig of the Rym4/Rym5 locus conferring resistance to the barley yellow mosaic virus complex (BaMMV/BaYMV), was exploited in order to develop SSR markers for practical barley breeding. Out of 57 SSR motifs found within this sequence, primers were designed and tested for the 5 SSRs with the highest repeat length. The polymorphic SSR marker QLB1 co-segregated with rym4 and rym5 phenotypes in respective high-resolution mapping populations developed for the construction of the original BAC contig. The primers targeted 2 sites located 756 bp and 5173 bp downstream of the translation initiation factor 4E (Hv-eIF4E). Physical linkage of the QLB1 marker to the Rym4/Rym5 locus was confirmed experimentally on Morex BAC 519J14, a seed BAC of Hv-eIF4E, and BAC 801A11, which is located proximally to Hv-eIF4E. QLB1 revealed 7 alleles in a set of 100 winter barley lines and cultivars. Five alleles were found within 673 advanced breeding lines derived from applied Polish winter barley breeding programmes, which corresponds to a PIC value of 0.684. No recombinants between Rym4/5 and QLB1 were detected, suggesting that QLB1 can be used efficiently in marker-assisted selection of the Hv-eIF4E-mediated bymovirus resistance.
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