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EN
The production of biomass by Yarrowia lipolytica on crude rapeseed oil and waste fatty acids and its characteristics were studied. The biomass obtained in both lipid media had high biological value in the range of 73-78%. The amount of essential aminoacids was in agreement with the FAO standards for fodder yeast, with lysine and phenylalanine which were present in the yeast biomass in higher amounts. The biomass contained 31,3-50,9% of protein and 11,9-28,1% of lipid depending on the yeast strain used. Yarrowia lipolytica ATCC 8661 turned out to be the most suitable strain for the production of SCP on lipid substrates.
EN
The production of Single Cell Protein in the batch culture from crude rapeseed oil and waste fatty acids using Y. lipolytica strains was studied. The effect of nitrogen sources, lipid concentration and pH value on the yeast growth and biomass yield was also investigated. Yarrowia lipolytica ATCC 8661 was the best strain for SCP production on both lipid substrates. The specific growth rate and biomass yield were m = 0,19 - 0,35 h-1 and Yx/s = 0,68 - 1,07 respectively, depending on the culture condition. With the above yeast strain it was possible to carry out the fermentation at low pH 3,5 without any significant lowering of kinetic and yield parameters.
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