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1

Genomic organization and nucleotide sequence of the repetitive DNA from Vicia sativa L.

100%
Lehmann P., Kozubek E.
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vol. 34
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issue 3
185-195
EN
The present study refers to the previously reported identification of a family of interspersed repeated sequence in Vicia sativa. It deals with the organisation, and potential of these sequences. A high degree of homology was found between repeats belonging to this family within both Vicia sativa and two other species, which indicates a conservative nature of these repeated sequences. Dot blot hybridization and computer analysis suggest that it is possible to express DNA fragments localized within the repeated sequences of the described family. The family of sequences, the object of these studies, has not been identified in the genome of Vicia sativa so far.
2

Electrophoretic seed albumin patterns in the Vicia sativa L. aggregate

75%
Przybylska J., Zimniak-Przybylska Z., Krajewski P.
Journal of Applied Genetics
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2000
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vol. 41
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issue 3
139-149
EN
Electrophoretic analysis of seed albumins covered 201 accessions representing six subspecies of Vicia sativa L.: nigra, amphicarpa, incisa, sativa, macrocarpa and cordata. At least five individuals of each accession were examined separately. All the taxa under study showed variation with respect to albumin banding patterns, which was due to differences between accessions and individual variation within accessions. The number of albumin bands distinguished in particular taxa varied from 13 to 19. The statistical analysis of the electrophoretic data consisted in hierarchical grouping by the UPGMA method based on EUCLIDEAN distances. In the case of subsp. nigra and subsp. sativa, accessions originating from North Africa tended to form a group showing some distinctness from the remaining accessions. As to relationships among the studied taxa, subsp. nigra and subsp. cordata were shown to be the most closely related and their affinities to other members of the V. sativa aggregate were decreasing in the following order: subsp. macrocarpa, subsp. sativa, subsp. amphicarpa, subsp. incisa. The obtained results are discussed with reference to taxonomic relationships among the members of the V. sativa aggregate.
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