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1

Exploration of genetic diversity among Xinjiang Triticum and Triticum polonicum by AFLP markers

100%
Akond M. A. S. M. G., Watanabe N., Furuta Y.
Journal of Applied Genetics
|
2007
|
vol. 48
|
issue 1
25-33
EN
Seventy-two Xinjiang Triticum and Triticum polonicum accessions were subjected to AFLP analyses to discuss the origin of Triticum petropavlovskyi. A total of 91 putative loci were produced by four primer combinations. Among them 56 loci were polymorphic, which is equivalent to 61.53 % of the total number of putative loci. Genetic diversity among 11 T. petropavlovskyi accessions was narrow due to the lowest number (32) of polymorphic loci among the wheat species. Forty four polymorphic loci were found in T. aestivum and T. compactum, whereas the highest polymorphism was observed in T. polonicum. On the basis of the UPGMA clustering and PCO grouping and genetic similarity estimates from the AFLPs, we noted that T. petropavlovskyi was more closely related to the Chinese accessions of T. polonicum than to T. polonicum from other countries. Two accessions of T. aestivum were grouped with T. petropavlovskyi in the UPGMA clustering. Both of them were similar to T. petropavlovskyi in respect of spike structure, i.e. the presence of awn, glume awn and also the presence of leaf pubescence. Six loci, which were commonly absent in Chinese T. polonicum, were also absent in almost all of the T. petropavlovskyi accessions. Findings of this study reduced the probability of an independent allopolyploidization event in the origin of T. petropavlovskyi and indicated a greater degree of gene flow between T. aestivum and T. polonicum leading to T. petropavlovskyi. It is most likely that the P-gene of T. petropavlovskyi hexaploid wheat was introduced from T. polonicum to T. aestivum via a spontaneous introgression or breeding effort.
2

Identification of powdery mildew resistance genes in common wheat (Triticum aestivum L. em. Thell.). XI. Cultivars grown in Poland

100%
Kowalczyk K., Hsam S. L. K., Zeller F. J.
Journal of Applied Genetics
|
1998
|
vol. 39
|
issue 3
225-236
EN
A collection of common wheat cultivars grown in Poland were analyzed for resistance to powdery mildew disease by using eleven differential isolates of Erysiphe graminis f. sp. tritici (Blumeria graminis). Among a total of 69 accessions, 48 cultivars possessed resistance which is attributed to known resistance genes present either individually or in a combination. Four cultivars were resistant to all the isolates used and another four cultivars revealed race-specific resistance which does not correspond to the response patterns of previously documented resistance. Resistance genes Pm2 and Pm6 in a combination were most widely distributed, and genes Pm3d, Pm4b, Pm5 and Pm8 were also postulated.
3

Genetic relationships among wheat genotypes, as revealed by microsatellite markers and pedigree analysis

100%
Sud S., Bains N. S., Nanda G. S.
Journal of Applied Genetics
|
2005
|
vol. 46
|
issue 4
375-379
EN
Genetic relationships among 20 elite wheat genotypes were studied using microsatellite markers and pedigree analysis. A total of 93 polymorphic bands were obtained with 25 microsatellite primer pairs. Coefficient of parentage (COP) values were calculated using parentage information at the expansion level of 5. The pedigree-based similarity (mean 0.115, range 0.00?0.53) was lower than the similarity assessed using microsatellite markers (mean 0.70, range 0.47?0.91). Similarity estimates were used to construct dendrograms by using the unweighted pair-group method with arithmetic averages (UPGMA). Clustering of genotypes in respect of marker-based similarity revealed two groups. Genotype PBW442 diverged and appeared as distinct from all other genotypes in both marker-based and pedigree-based analysis. The correlation of COP values with genetic similarity values based on microsatellite markers is low (r = 0.285, p < 0.05). The results indicate a need to develop wheat varieties with a diverse genetic background and to incorporate new variability into the existing wheat gene pool.
4

Somaclonal variation in winter wheat (Triticum aestivum L.): frequency, occurrence and inheritance

100%
Cheng X. Y., Gao M. W., Liang Z. Q., Liu G. Z.
Journal of Applied Genetics
|
1998
|
vol. 39
|
issue 1
59-72
EN
Plants were regenerated from immature embryo cultures of 35 winter wheat genotypes. General responses of regenerated plants were investigated and a total of 7142 R2 spike lines from 1593 Rl plants were assessed in the field for somaclonal variants in 1985/86, 1986/87 and 1987/88. Selected variants were studied for their possible genetic inheritance. From regenerated plantlets, 81% survived and 63% produced fertile plants. Forms with reduced plant height, length of spike and other morphological abnormalities were found in this progeny. Populations of Rl plants were highly variable due mainly to the physiological disturbances resulting from the in vitro process. Overall somaclonal variation frequencies were 14.2% per plant basis and 5.3% per R2 spike basis. The variants were similar in the three different R2 generations with predominant variants being negative in plant height, maturity, awns, spike type and plant type. Both uniform R2 variant families and spike lines were found in addition to the segregating variants which constituted the majority. On average, in a variant family or line, 18% and 14% of their component lines and plants were variants, respectively. Inheritability was demonstrated for the uniform variant families and spike lines as well as segregated variants. Of those 134 selections, about 70% were classified as inheritable. Both recessive and dominant gene mutations at one, two or three loci were evident in some variants as suggested by the segregating data.
5

QTL mapping of 1000-kernel weight, kernel length, and kernel width in bread wheat (Triticum aestivum L.)

100%
Ramya P., Chaubal A., Kulkarni K., Gupta L., Kadoo N., Dhaliwal H. S., Chhuneja P., Lagu M., Gupta V.
Journal of Applied Genetics
|
2010
|
vol. 51
|
issue 4
421-429
EN
Kernel size and morphology influence the market value and milling yield of bread wheat (Triticum aestivum L.). The objective of this study was to identify quantitative trait loci (QTLs) controlling kernel traits in hexaploid wheat. We recorded 1000-kernel weight, kernel length, and kernel width for 185 recombinant inbred lines from the cross Rye Selection 111 ? Chinese Spring grown in 2 agro-climatic regions in India for many years. Composite interval mapping (CIM) was employed for QTL detection using a linkage map with 169 simple sequence repeat (SSR) markers. For 1000-kernel weight, 10 QTLs were identified on wheat chromosomes 1A, 1D, 2B, 2D, 4B, 5B, and 6B, whereas 6 QTLs for kernel length were detected on 1A, 2B, 2D, 5A, 5B and 5D. Chromosomes 1D, 2B, 2D, 4B, 5B and 5D had 9 QTLs for kernel width. Chromosomal regions with QTLs detected consistently for multiple year-location combinations were identified for each trait. Pleiotropic QTLs were found on chromosomes 2B, 2D, 4B, and 5B. The identified genomic regions controlling wheat kernel size and shape can be targeted during further studies for their genetic dissection.
6

Wheat anther culture as affected by various cultural changes and supplements

100%
Simonson R. L., Beanziger P. S., Gustafson V. D.
Journal of Applied Genetics
|
1997
|
vol. 38
|
issue 4
381-392
EN
With the numerous improvements in cereal tissue and wheat anther culture, it is necessary to determine which of the improvements should be combined for optimal response. This study was conducted using one highly responsive cultivar of wheat (Triticum aestivum L. cv. Pavon 76) to test the effectiveness of pre-culture cold treatment (0 or 3-7 days at 5oC) of anthers, five initiation basal media, and various changes in Murashige-Skoog regeneration media. A cold pre-culture treatment was inhibitory for all initiation media for embryoid initiation. Of the initiation media, P1, 85D12, and N6 were similar for embryoid initiation (0.80 to 0.90 embryoids/anther) without a cold pre-culture treatment. Plant regeneration was improved by the addition of amino acids or glucose, increased sucrose concentration, filter sterilizing the medium, and altering plant growth regulator concentrations. P1 medium which is normally used for embryoid initiation was also beneficial for improving plant regeneration. Ethylene inhibitors were generally not beneficial.
7

Identification of powdery mildew resistance genes in common wheat (Triticum aestivum L. em. Thell.). X. Cultivars grown in Belarus and neighbouring countries

100%
Gordei S. I., Hsam S. L. K., Zeller F. J.
Journal of Applied Genetics
|
1998
|
vol. 39
|
issue 1
1-8
EN
Sixty-six wheat cultivars grown in Belarus, Poland, Russia and the Ukraine were tested for mildew response to a collection of 11 different isolates of Erysiphe graminis DC f. sp. tritici Marchal. Nineteen cultivars have shown a susceptible reaction and eighteen were characterized by susceptible or intermediate responses. Fourteen cultivars revealed isolate-specific response patterns that could be attributed to major known resistance genes or gene combinations. Twelve cultivars have one documented gene: Pm5 in eight cultivars, Pm2 in two cultivars and Pm8 also in two cultivars. One cultivar has two genes (Pm2 + Pm6), while another cultivar carries a combination of three genes (Pm1 + Pm2 + Pm6). Fifteen cultivars were characterized by response patterns not documented so far or by a known resistance response combined with an undocumented resistance. Apparently three cultivars with the T1BL.1RS wheat-rye translocation have a gene suppressing the Pm8 mildew resistance. One cultivar was resistant to all the used isolates. Its resistance might be conditioned by an unknown major gene or combination of genes.
8

Genetics of durable resistance to leaf rust and stripe rust of an Indian wheat cultivar HD2009

100%
Khanna R., Bansal U. K., Saini R. G.
Journal of Applied Genetics
|
2005
|
vol. 46
|
issue 3
259-263
EN
The Indian bread wheat cultivar HD2009 has maintained its partial resistance to leaf rust and stripe rust in India since its release in 1976. To examine the nature, number and mode of inheritance of its genes for partial leaf rust and stripe rust resistance, this cultivar was crossed with cultivar WL711, which is susceptible to leaf rust and stripe rust. The F1, F2, F3 and F5 generations from this cross were assessed separately for adult plant disease severity under artificial epidemic of race 77-5 of leaf rust and race 46S119 of stripe rust. Segregation for rust reaction in the F2, F3 and F5 generations indicated that resistance to each of these rust diseases is based on 2 genes, each with additive effects. Although the leaf rust resistance of HD2009 is similar in expression to that conferred by the gene Lr34, but unlike the wheats carrying this gene, cultivar HD2009 did not show leaf tip necrosis, a morphological marker believed to be tightly linked to the leaf rust resistance gene Lr34. Thus, the non-hypersensitive resistance of HD2009 was ascribed to genes other than Lr34.
9

Production and evaluation of doubled haploid wheat lines

100%
Pauk J., Kertesz Z.
Journal of Applied Genetics
|
1997
|
vol. 38
|
issue 4
425-435
EN
F1, F2 populations as well as F3 and F4 wheat lines coming from more than 300 cross combinations have been used for androgenic wheat studies and more than 5000 haploid plants were produced. Promising doubled haploid lines were tested together with lines produced in the conventional way in yield trials. The two best doubled haploid lines overyielded the standard. On the basis of results related to the general combining ability it is advisible to cross parents with a good combining ability for characters such as high percentage of responding anthers and embryo/callus induction with parents indicating a good combining ability for green plant production. In this way ideal lines can be obtained for doubled haploid production.
10

Agronomic performance of wheat doubled haploids produced through wheat x maize crosses

100%
Guzy-Wrobelska J., Szarejko I., Nawrot M., Madajewska M.
Biotechnologia
|
2001
|
issue 2
72-79
EN
Wheat x maize crosses are used as a method of wheat doubled haploid (DH) production, alternative to anther culture. The study was conducted to compare the agronomic performance of DH lines produced through wide crosses with lines obtained by the single seed descent (SSD) method from the same plant material. F1 progeny of spring wheat varieties: Eta x Sigma (both Polish) and Eta x Darkhan 15 (Mongolian) were used for DH and SSD production. Doubled haploids (DH3, DH4) and SSD (F5) lines from both genotypes were evaluated for plant height, spike length, tillering, grain weight and number per plant and 1000 grain weight in a randomised, three replicated experiment. Mean performance, coefficient of variation and frequency distribution of DH and SSD lines were similar for most of the analysed traits. The comparison of the best 10% of DH and SSD lines from both crosses (selected on the basis of grain weight per plant) confirmed their similar performance. For the majority of the analysed characters, the best DH lines derived from both DH populations did not differ from better parents and check varieties. Ten percent of Eta x Sigma DH lines performed significantly better for 1000 grain weight than both parental genotypes, heterotic F1 and check varieties. The results indicate that maize pollination system is an efficient method of producing high yielding homozygous lines of wheat and it may be recommended for a wide use in wheat breeding programmes.
11

Molecular cytogenetic evaluation of chromosome instability in Triticum aestivum?Secale cereale disomic addition lines

88%
Szakacs E., Molnar-Lang M.
Journal of Applied Genetics
|
2010
|
vol. 51
|
issue 2
149-152
EN
The genetic stability of wheat/rye ('Chinese Spring'/'Imperial') disomic addition lines was checked using the Feulgen method and fluorescent in situ hybridization (FISH). Feulgen staining detected varying proportions of disomic, monosomic, and telosomic plants among the progenies of the disomic addition lines. The greatest stability was observed for the 7R addition line, while the most unstable lines were those with 2R and 4R additions. Chromosome rearrangements were also detected using FISH. Based on the specific hybridization patterns of repetitive DNA probes pSc119.2 and (AAC)5, as well as ribosomal DNA probes (5S and 45S), isochromosomes were identified in the progenies of 1R and 4R addition lines. The results draw attention to the importance of continuous cytological checks on basic genetic materials by using FISH, because this method reveals chromosome rearrangements that could not be detected either with the conventional Feulgen staining technique or with molecular markers.
12

Undescribed wheat gene for partial leaf rust and stripe rust resistance from Thatcher derivatives RL6058 and 90RN2491 carrying Lr34

88%
Agarwal S., Saini R. G.
Journal of Applied Genetics
|
2009
|
vol. 50
|
issue 3
199-204
EN
Inheritance of partial leaf rust and stripe rust resistance of a Thatcher wheat 90RN2491, earlier reported to carry two doses of the gene pair Lr34-Yr18 and the reference line RL6058 (6*Thatcher/PI58548) for the Lr34-Yr18 gene pair was studied against predominant and highly virulent Indian races. Thatcher derivatives 90RN2491 and RL6058 were intercrossed as well as crossed with the leaf rust and stripe rust susceptible Indian cultivar WL711. The F1, F2 and F3 generations from these crosses were assessed for rust severity against leaf rust race 77-5 and stripe rust race 46S119. The F2 and F3 generations from the crosses of RL6058 and 90RN2491 with WL711, segregated 15 resistant : 1 susceptible (F2) and 7 homozygous resistant : 8 segregating : 1 homozygous susceptible (F3) ratios, respectively, both for leaf rust and stripe rust severity. Therefore, partial resistance against each of the leaf rust and stripe rust races in both RL6058 and 90RN2491 is ascribed to two independently inherited dominant genes. One of the two genes for leaf rust and stripe rust resistance in 90RN2491 and RL6058 is Lr34 and the linked gene Yr18, respectively. The second leaf rust resistance gene in both the Thatcher lines segregated independently of stripe rust resistance. Therefore, it is not Lr34 and it remains unidentified.
13

Inheritance of leaf rust resistance in wheat lines carrying Aegilops speltoides Tausch. translocation in Chinese Spring background

88%
Bansal U. K., Saini R. G., Khanna R.
Journal of Applied Genetics
|
2008
|
vol. 49
|
issue 2
141-145
EN
The genetic basis of seedling and adult-plant leaf rust resistance was analysed in wheat lines CS 2A/2M 4/2 and CS 2D/2M 3/8, which are reference lines for the leaf rust resistance gene Lr28. Some seedlings of CS 2A/2M 4/2 were susceptible to Indian Puccinia triticina (Pt) pathotypes 77-1, 77-2 and 77-5. These susceptible seedlings exhibited resistance at the adult-plant growth stage. In contrast, CS 2D/2M 3/8 showed resistance to all Pt pathotypes both at the seedling and adult-plant growth stages. The analysis of inheritance in the susceptible plants of CS 2A/2M 4/2 (CS 2A/2M 4/2 APR selection) and CS 2D/2M 3/8 against Pt 77-5 (the frequently occurring Pt pathotype from the Indian subcontinent), indicated that line CS 2D/2M 3/8 was fixed for a dominant gene, presumed to be Lr28, whereas line CS 2A/2M 4/2 was heterogeneous for Lr28. The adult-plant resistance in the CS 2A/2M 4/2 APR selection was conferred by an unknown recessive gene.
14

Microsatellite mapping of the genes for brittle rachis on homoeologous group 3 chromosomes in tetraploid and hexaploid wheats

88%
Watanabe n., Fujii Y., Kato N., Ban T., Martinek P.
Journal of Applied Genetics
|
2006
|
vol. 47
|
issue 2
93-98
EN
The brittle rachis character, which causes spontaneous shattering of spikelets, has an adaptive value in wild grass species. The loci Br1 and Br2 in durum wheat (Triticum durum Desf.) and Br3 in hexaploid wheat (T. aestivum L.) determine disarticulation of rachides above the junction of the rachilla with the rachis such that a fragment of rachis is attached below each spikelet. Using microsatellite markers, the loci Br1, Br2 and Br3 were mapped on the homoeologous group 3 chromosomes. The Br2 locus was located on the short arm of chromosome 3A and linked with the centromeric marker, Xgwm32, at a distance of 13.3 cM. The Br3 locus was located on the short arm of chromosome 3B and linked with the centromeric marker, Xgwm72 (at a distance of 14.2 cM). The Br1 locus was located on the short arm of chromosome 3D. The distance of Br1 from the centromeric marker Xgdm72 was 25.3 cM. Mapping the Br1, Br2 and Br3 loci of the brittle rachis suggests the homoeologous origin of these 3 loci for brittle rachides. Since the genes for brittle rachis have been retained in the gene pool of durum wheat, the more closely linked markers with the brittle rachis locus are required to select against brittle rachis genotypes and then to avoid yield loss in improved cultivars.
15

A simplified AFLP method for fingerprinting of common wheat (Triticum aestivum L.) cultivars

88%
Tyrka M.
Journal of Applied Genetics
|
2002
|
vol. 43
|
issue 2
131-143
EN
The simplified AFLP method was developed and evaluated for identification and genetic diversity studies of wheat cultivars. Selective primers exploited in AFLP assay based on a single cutting enzyme PstI (PstIAFLP) generated total of 111 robust fragments, including 67 (60%) monomorphic and 12 (11%) cultivar-specific markers. Average similarity between 15 cultivars was 0.650, and varied from 0.293 (?Hope? vs. ?Aurora?) to 0.865 (?Norman? vs. ?Hornet?). Mean similarities within groups of winter wheat cultivars with and without 1BL/1RS chromosome were 0.713 and 0.685, respectively. A higher variation was found in the group of spring wheats: 0.677. The obtained results confirm the usefulness of the proposed modification of the AFLP technique for diversity studies and identification of common wheat cultivars.
16

Mapping QTLs for pre-harvest sprouting tolerance on chromosome 2D in a synthetic hexaploid wheat?common wheat cross

88%
Xiao-bo R., Xiu-jin L., Deng-cai L., Jia-li W., You-liang Z.
Journal of Applied Genetics
|
2008
|
vol. 49
|
issue 4
333-341
EN
Based on segregation distortion of simple sequence repeat (SSR) molecular markers, we detected a significant quantitative trait loci (QTL) for pre-harvest sprouting (PHS) tolerance on the short arm of chromosome 2D (2DS) in the extremely susceptible population of F2 progeny generated from the cross of PHS tolerant synthetic hexaploid wheat cultivar 'RSP' and PHS susceptible bread wheat cultivar '88-1643'. To identify the QTL of PHS tolerance, we constructed two SSR-based genetic maps of 2DS in 2004 and 2005. One putative QTL associated with PHS tolerance, designated Qphs.sau-2D, was identified within the marker intervals Xgwm261-Xgwm484 in 2004 and in the next year, nearly in the same position, between markers wmc112 and Xgwm484. Confidence intervals based on the LOD-drop-off method ranged from 9 cM to 15.4 cM and almost completely overlapped with marker interval Xgwm261-Xgwm484. Flanking markers near this QTL could be assigned to the C-2DS1-0.33 chromosome bin, suggesting that the gene(s) controlling PHS tolerance is located in that chromosome region. The phenotypic variation explained by this QTL was about 25.73%-27.50%. Genotyping of 48 F6 PHS tolerant plants derived from the cross between PHS tolerant wheat cultivar 'RSP' and PHS susceptible bread wheat cultivar 'MY11' showed that the allele of Qphs.sau-2D found in the 'RSP' genome may prove useful for the improvement of PHS tolerance.
17

Cytogenetic effects of Fusarium mycotoxins on root tip cells of rye (Secale cereale L.), wheat (Triticum aestivum L.) and field bean (Vicia faba L. var. minor)

88%
Packa D.
|
|
issue 3
259-272
EN
The effects of fusarial toxins: DAS, T 2, DON, 3 Ac DON, MON and ZEA, on actively dividing root tip cells of rye, wheat and field bean were investigated. Three concentrations: 1, 5 and 10 mg mL-1 were applied for 24 hours. Nuclei and chromosomes were stained using the Feulgens method. It has been found that trichothecene mycotoxins (DAS, T 2, DON, 3 Ac DON) had a profound effect on mitosis, as they decreased the mitotic index, produced excessive condensation of pro and metaphase chromosomes, C metaphases and C anaphases, and caused an accumulation of metaphases. The main effect of trichothecene mycotoxins, probably caused via their influence on protein synthesis, was abnormal functioning of the mitotic spindle.
18

Influence of genetic and environmental factors on anther culture response of wheat

88%
Zhou H., Konzak C. F.
Journal of Applied Genetics
|
1997
|
vol. 38
|
issue 4
393-406
EN
The influences of genetic and environmental factors on the anther culture responses of wheat were investigated. Significant differences for callus induction, plant regeneration, and green plant percentages were observed when the nucleus of Triticum aestivum L. cv. Selkirk was transferred to ten alien cytoplasms by substitution backcrosses. In most cases, the alien cytoplasms decreased anther culture responses, but sometimes they were as good as or better than the T. aestivum cytoplasm. Significant within-genotype variation for anther culture responses were observed for wheat varieties Chris, Yecora Rojo, WA7176 and Edwall, indicating genetic heterogeneity in the present commercial cultivars, and potential for improving anther culture responses by in vitro prescreening. When five genotypes (Chris, Pavon 76, Butte 86, WA6916, and Edwall) were cultured across three (potato-4 liquid, 100 g L-1 ficoll-supplemented, and 6 g L-1 agar-solidified) induction media, the liquid and ficoll-containing media were 10 to 15 times more productive than the agar-solidified medium. Whereas, the ficoll medium was not significantly different from the liquid medium. Several low concentration starch media appeared promising to replace current induction media. The starch media sustained the high-callus-induction properties of the liquid medium, while improving callus aeration similar to that observed on solid media, resulting in markedly higher plant regeneration and green plant percentages.
19

Saturation and mapping of a major Fusarium head blight resistance QTL on chromosome 3BS of Sumai 3 wheat

88%
Zhou M.-P., Hayden M. J., Zhang Z.-Y., Lu W.-Z., Ma H.-X.
Journal of Applied Genetics
|
2010
|
vol. 51
|
issue 1
19-25
EN
Fusarium head blight (FHB) is a destructive disease in wheat. The major quantitative trait locus (QTL) on 3BS from Sumai 3 and its derivatives has been used as a major source of the resistance to FHB worldwide, but the discrepancy in reported location of the major QTL could block its using in map based cloning and marker assisted selection. In this study, Chinese Spring-Sumai 3 chromosome 3B substitution line was used as resistant parent of the mapping population to reduce the confounded effect of genetic background in Sumai 3. The major QTL region was saturated with the Sequence Tagged Microsatellite (STM) and Sequence Tagged Site (STS) markers. A linkage map of chromosome 3B with 36 markers covering a genetic distance of 112.4 cM was constructed. Twelve new markers were inserted into the chromosome region where the major QTL was located. The average interval distance between markers was 1.5 cM. Multiple QTL Models (MQM) mapping indicated that the major QTL was located in the interval of Xgwm533 ? Xsts9-1, and explained 45.6% of phenotypic variation of the resistance to FHB. The SSR (simple sequence repeat) marker Xgwm533 and STM marker Xstm748tcac are closely linked to the major QTL.
20

Gene transfer from Aegilops ventricosa to Triticum aestivum

75%
Tyrka M., Stefanowska G., Brzezinski W.
Biotechnologia
|
2001
|
issue 2
57-62
EN
RAPD (Randomly Amplified Polymorphic DNA) and glutenin SDS-PAGE analyses were performed on wheat hybrid strains derived from multiple crosses of hybrids between Ae. ventricosa and T. durum with hexaploid wheat. We found Aegilops specific bands (G03580 and T02990) in two hybrid strains (VGPB and VGPAA) using RAPD method. The presence of Ae. ventricosa specific fraction of glutenin was shown in one wheat hybrid strain (VGPP).
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