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Analysis of the wheat endosperm transcriptome

100%

Laudencia-Chingcuanco D. L., Stamova B. S., Lazo G. R., Cui X., Anderson O. D.

Journal of Applied Genetics

2006

vol. 47

issue 4

287-302

Among the cereals, wheat is the most widely grown geographically and is part of the staple diet in much of the world. Understanding how the cereal endosperm develops and functions will help generate better tools to manipulate grain qualities important to end-users. We used a genomics approach to identify and characterize genes that are expressed in the wheat endosperm. We analyzed the 17 949 publicly available wheat endosperm EST sequences to identify genes involved in the biological processes that occur within this tissue. Clustering and assembly of the ESTs resulted in the identification of 6 187 tentative unique genes, 2 358 of which formed contigs and 3 829 remained as singletons. A BLAST similarity search against the NCBI non-redundant sequence database revealed abundant messages for storage proteins, putative defense proteins, and proteins involved in starch and sucrose metabolism. The level of abundance of the putatively identified genes reflects the physiology of the developing endosperm. Half of the identified genes have unknown functions. Approximately 61% of the endosperm ESTs has been tentatively mapped in the hexaploid wheat genome. Using microarrays for global RNA profiling, we identified endosperm genes that are specifically up regulated in the developing grain.

Functional genomic tools for Arabidopsis available at the MPIZ

100%

Dekker K., Sorensen T. R., Weisshaar B., Saedler H.

2003

issue 4

19-31

This article summarizes the activities at the Max Planck Institute for Plant Breeding Research (Max-Planck-Institut f?r Z?chtungsforschung, MPIZ) in the area of ?Arabidopsis genomics?. We describe the status of three Arabidopsis thaliana genomic projects at the MPIZ: 1) The Gene Knock-Out Facility ZIGIA (Zentrum zur Identifikation von Genfunktionen durch Insertionsmutagenese bei A. thaliana, Center for Functional Genomics in A. thaliana) using lines tagged with the maize transposon En/Spm, 2) the GABI-Kat project that provides sequence indexed T-DNA tagged lines and 3) the GABI-MASC project that creates mapping tools based on single nucleotide polymorphisms (SNP) for efficient assessment of natural diversity in A. thaliana. The materials and tools developed by these projects are publicly available and used worldwide by scientist to explore the frontiers of plant sciences.

The transpositional elements as a tool for plant genome mapping

63%

Wochniak P., Malepszy S.

Biotechnologia

1994

issue 3

15-29

This work aims at reviewing current progress in the field of plant transposble elements, especially those described as Ac/Ds and En/dSpm systems, first discovered in maize.We gave the molecular characteristics of plant transposones and the rules of their behaviour within a genome.The procedure for a particular gene mapping and for mapping of many genes responsible for biochemical pathway were cited.In comparison with other genome mapping methods the advantages and drawbacks of "gene tagging" were envisaged.The enclosed tables provide many documental examples of plant genes identification via "gene tagging" method.

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2 Biotechnologia

1 Journal of Applied Genetics

1 2006

1 2003

1 1994

1 Anderson O. D.

1 Cui X.

1 Dekker K.

1 Laudencia-Chingcuanco D. L.

1 Lazo G. R.

1 Malepszy S.

1 Saedler H.

1 Sorensen T. R.

1 Stamova B. S.

1 Weisshaar B.

1 Wochniak P.

Search results

Analysis of the wheat endosperm transcriptome

Functional genomic tools for Arabidopsis available at the MPIZ

The transpositional elements as a tool for plant genome mapping