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1

Epithelial cell cultures as a model system to study the transepithelial transport

100%
Grajek W., Olejnik A., Staniaszek K.
Biotechnologia
|
2006
|
issue 2
148-165
EN
Epithelial cell lines are commonly accepted model system to determine intestinal permeability and transepithelial transport. The application of Caco-2 human epithelial cell culture can be used to study bioavailability of drug and food compounds. This culture grows on porous membranes and forms a monolayer of well differentiated cells joined by intracellular tight junctions. This system provides selective barrier to study passive and active transport of different molecules. The Caco-2 cells posses some enzymes and transport systems similar to intestinal epithelium in vivo, however, these proteins are expressed to a lesser extent compared to normal enterocytes. In this paper, the advantages and limitations of Caco-2 model are discussed.
2

Ion transport in the colon of rats experimentally infected with liver fluke (Fasciola hepatica)

100%
Kosik-Bogacka D. I., Kolodziejczyk L.
Folia Biologica
|
2004
|
vol. 52
|
issue 3-4
243-246
EN
The effect of liver fluke infection (Fasciola hepatica) on ion transport in the rat proximal colon was evaluated with electrophysiological methods using an Ussing apparatus. Rats were orally infected with F. hepatica metacercariae. The experimental groups of rats at 4 weeks post-infection (wpi) were in the acute stage of fasciolosis, while those at 7, 10 and 13 wpi represented the chronic phase of liver fluke infection. The control group comprised uninfected rats. The experiment consisted of recording transepithelial electrical potential difference (PD) and transepithelial electrical resistance (R) in the colon of the rats. The results revealed reduced PD levels in the colon of rats, especially during the acute phase of fasciolosis. During its chronic phase, a gradual growth in the intestinal transepithelial potential difference was found. Rat fasciolosis did not cause changes in colon sensitivity to mechanical stimulation or in the value of electrical resistance. Only at 13 wpi was resistance found to have dropped. The results have demonstrated that experimental fasciolosis leads to activation of inflammatory mediators and thus to stimulation of nerve fibres, which modifies ion transport in the epithelium of the host large intestine.
3

Theoretical approach to the estimation of water optical properties from the radiance field in the sea

88%
Matciak M.
Oceanological Studies
|
2001
|
vol. 30
|
issue 1-2
99-111
EN
A general problem of determining water inherent optical properties from natural light radiance distribution in the sea and its depth dependency is analysed. Expressions for light-beam attenuation coefficient of practical significance are obtained. The presence of internal sources of light energy like fluorescence and Raman scattering by water molecules is included in consideration.
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