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EN
Experiments on attached-growth circulating reactor were conducted to determine the impact of variable heat energy of the reactor packing on nitrification rate. There was a possibility of regulation the packing temperature. The research was carried out for biofilm temperatures from 10oC to 35oC at ambient temperature of 10oC. The increase in packing temperature from 10oC to 30oC caused the increase in nitrification rate from 3.57 gN?m-2?d-1 to 10.1 gN?m-2?d-1. The increase in temperature over 30oC caused the decrease in process rate. Appointed values of temperature coefficient ? for nitrification decreased by increasing in heat energy of packing. The supply of heat energy to biofilm directly through the reactor packing permitted to improve the nitrification effectiveness.
EN
Responses of the African weaver ants (Oecophylla longinoda) to nest damages were studied in the field in Nigeria. During the wet season, the ants responded to nest damages almost unexceptionnally by a quick onset of nest-repairing behaviour. The latencies to the start of nest-repairing activities (LN) did not depend on the size of the damage, but they were significantly shorter during the night, and positively correlated with ambient temperature. During the dry season, the ants responded to large nest damages mainly by abandonning the nest. In the case of medium size damages, the onset of nest-repairing behaviour was equally rapid as during the wet season, but in the case of small damages it was sometimes greatly delayed (up to three hours). The values of LN did not differ between the nighttime and the remaining times of the day, and they were not correlated with ambient temperature.
EN
The 7-days shift of M. funereus larvae, from nature to a constant temperature of 23?C led to changes in midgut and brain protein quality and quantity. The changes in midgut protein profiles are characterized by an intensified protein band Mr of 29 kD, the absence of protein Mr of 22 kD and less intense bands Mr of 8.5-2.5 kD. Electrophoretic patterns of brain proteins showed less intense Mr of 66-2.5 kD protein bands.
EN
Previous ex vivo experiments by others suggest that elevated body temperature can prime the respiratory burst of human neutrophils. The mechanism of the priming phenomenon induced by temperature has not been addressed so far. Furthermore, the priming temperature range was not defined. In the present study we explored, under in vitro conditions, the influence of febrile.range temperatures on reactive oxygen species (ROS) generation by human peripheral blood neutrophils. ROS production was measured using whole.blood luminol.dependent chemiluminescence. Two elements of signal transduction pathways, calcium and p38 mitogen.activated protein kinase alpha (p38MAPK alpha), frequently underlying neutrophil priming were also examined. Calcium levels in the cytosol of resting and fMLP.stimulated isolated neutrophils were measured with the Fura.2AM spectrofluorimetric method. The activity of p38MAPK alpha was assessed indirectly with a specific inhibitor of the kinase, SB 203580. The study revealed a priming effect at 38?C toward human peripheral blood neutrophil ROS production. Any con. comitant effect on calcium response was not observed. Instead, experiments with SB 203580, a specific inhibitor of p38MAPK alpha, pointed to an increased activity of the kinase as a molecular background of temperature.induced priming. However, the priming effect of temperature was confined to 38?C, while higher temperatures proved to exert no effect (39 and 40?C) or even inhibited ROS generation by neutrophils (43?C). Our study suggests a heterogeneous influence of temperature on human neutrophil functioning, including the prim. ing of the cells by a low.febrile.range temperature. It also suggests a p38MAPK alpha dependent molecular background of the priming phenomenon.
EN
Higher air temperature in summer causes a significant reduction in fertility in cattle. Increase in female body temperature during the period of reproduction by only 2EC, also known as hyperthermia, leads to disturbances in the functioning of the female reproductive system, oocytes maturation, fertilization and embryos development. Particularly sensitive to high temperatures are embryos in the first and second day after fertilization (thermosensitive), but just at third till fifth day after fertilization their resistance to thermal stress significantly increases. Morula-stage and blastocyst-stage bovine embryos are insensitive to elevated temperatures (thermoresistant). Most probably this is due to the increasing number of cells within the embryo and the capacity to activate defense mechanisms based on the synthesis of various factors providing resistance to high temperatures. These factors include heat shock protein 70 (HSP70), antioxidants such as glutathione, and IGF-1. One of the responses of the embryo to elevated temperature is the induction of apoptosis, which is associated with the activation of embryonic genome. Owing to the apoptosis, cells damaged by high temperature may be eliminated from the embryo, which increases their chance of survival. Precise examination of the mechanisms responsible for the development of thermotolerance of preimplantation bovine embryos will enable their protection from the consequences of elevated temperature. The aim of this review is to summarise experiments in which in vitro embryo production system was used to estimate the influence of elevated temperature on cattle fertility.
EN
Two species of blue-green algae have been studied under laboratory conditions in several combinations of salinity (0, 8, 16, 24 PSU), light (20, 70, 120, 170 muE m^-2 s^-1) and temperature (20, 25, 30, 35 ?C). Temperature within the range of 30-35 ?C and light intensity of 170 mE m^-2 s^-1 were the optimum to achieve a maximum growth effect in the two species examined. Salinity as an environmental factor showed that these two tested species are fresh water in origin with some tolerance to increased salinity concentration. The best growth rate for Anabaena flos-aquae was stated in 0 PSU medium for all temperature and light conditions. For Nostoc sp. div. medium with 8 PSU was the optimum one.
EN
Long-term storage of shoots or nodal buds in vitro, in conditions which retard their growth, may be an alternative or supplementary method in the protection of plant resources and in the production of planting material for elite mother stocks. In this paper we describe the most important factors which influence the survival and further behaviour of stored explants.
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