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Effects of competitive pentathlon training on the antioxidant defence components

100%

Lewandowski P., Hübner-Woźniak E.

Study aim: To assess the effects of training on the activities of antioxidant enzymes in erythrocytes and on the total plasma antioxidant status in competitive pentathletes.Material and methods: A group of 10 senior male pentathletes (P) and of 10 sedentary male subjects (S) participated in the study. Blood was withdrawn from the antecubital vein in the morning, in the preprandial state. The activities of superoxide dismutase (SOD), glutathione reductase (GR) and catalase (CAT) were determined in erythrocyte haemolisates, that of glutathione peroxidase (GPX) in whole blood haemolysate, and the total antioxidant status (TAS) in plasma.Results: The activities of all enzymes were significantly (p<0.05 - 0.001) higher in P than in S group while no significant between-group difference was found for TAS.Conclusions: The pronounced enzymatic antioxidative potential and oxidative stress defence observed in athletes practicing modern pentathlon may be attributed to their extensive training.

Hyperhomocysteinemia’s effect on antioxidant capacity in rats

88%

Filip C., Albu E., Zamosteanu N., Irina M., Silion M., Jerca L., Gheorghita N., Costel M.

Open Medicine

2010

vol. 5

issue 5

620-626

Hyperhomocysteinemia represents elevated homocysteine (Hcys) concentrations in blood above the normal range. In humans, the normal range of homocysteine is 5.0–15.9 mM/ml. High levels of homocysteine disturb the normal epithelial functions and correlate with cardiovascular diseases even at slightly increased concentrations. In homocysteine metabolism, vitamins play an important role. The mechanism through which homocysteine triggers these effects is not yet elucidated, but the involvement of reactive species may be the answer. It is not known whether the intra- or extracellular antioxidant system is more affected by elevated homocysteine levels. We studied the effects of hyperhomocysteinemia on the intra- and extracellular antioxidant defense systems in two different types of diet in rats. Type I was food with low folic acid and vitamin B12 content and type II was food with normal amounts of these two vitamins. Hyperhomocysteinemia was experimentally induced by oral administration of methionine 2 mg/kg body weight, single daily dose, for a 15-day period. Plasma concentrations of homocysteine were measured using an HPLC method. In the response of the intracellular antioxidant defense system against hyperhomocysteinemia, we determined the activities of superoxide dismutase (SOD) and glutathione peroxidase (GPx) in red blood cells, using RANDOX kits for manual use. For the extracellular response we determined the plasma total antioxidant status (TAS) also using a RANDOX kit for manual use. Our data show that methionine load induces hyperhomocysteinemia despite normal vitamin supply in rats. SOD activity rose with simultaneous decrease in GPx activity independently of diet; this might suggest that the intracellular defense system was disturbed by the rise in homocysteine level. TAS decrease suggests that the extracellular antioxidant defense was also affected. We assume that hyperhomocysteinemia is directly linked to reactive species generation and the intracellular space seems to be more affected than the extracellular one.

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1 Biomedical Human Kinetics

1 Open Medicine

2 2010

1 Albu E.

1 Costel M.

1 Filip C.

1 Gheorghita N.

1 Hübner-Woźniak E.

1 Irina M.

1 Jerca L.

1 Lewandowski P.

1 Silion M.

1 Zamosteanu N.

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Effects of competitive pentathlon training on the antioxidant defence components

Hyperhomocysteinemia’s effect on antioxidant capacity in rats