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Selective sorption of uric acid by novel molecularly imprinted polymers

100%
Leshchinskaya A. P., Polyakova I. V., Groshikova A. R., Pisarev O. A., Panarin E. F.
Molecular Imprinting
|
2012
|
vol. 1
17-26
EN
The polymers based on ethylene glycol dimethacrylate (EGDMA) and dimethylaminoethyl methacrylate (DMAEMA) and molecularly imprinted with uric acid (UA), UA-MIPs, were successfully synthesized. The binding activity of UA-MIPs towards UA was studied in depth using batch methods. The optimized sorbent UA-MIP-7-16 was synthesized; this is an EGDMAcrosslinked system containing 16 mol% of UA as the template. The character of binding between UA and UA-MIP-7-16 was studied using xanthine as a reference substance, since its chemical structure is similar to that of UA. The studies of equilibrium sorption of UA and xanthine from model aqueous solutions by the imprinted sorbent demonstrate the predominance of specific UA sorption. The sorption kinetic data were analyzed using the Boyd model and shell and core. Selectivity of UAMIP- 7-16 was further demonstrated by biochemical analysis of serum containing UA and other components conducted before and after sorption. UA-MIP-7-16 showed high recognition selectivity and affinity towards the template molecule (UA).
2
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Cloud point extraction-preconcentration and flame atomic absorption spectrometric determination of low levels of zinc in water and blood serum samples

88%
Shokrollahi A., Shamsipur M., Jalali F., Nomani H.
Open Chemistry
|
2009
|
vol. 7
|
issue 4
938-944
EN
A simple, sensitive and selective flotation method is described for the preconcentration and atomic absorption spectrometric determination of zinc ion in water and blood samples. At a solution pH of 5.2, 4-(2-pyridylazo-resorcinol) and Triton X-114 were used as hydrophobic ligand and non-ionic surfactant, respectively. The chemical variables affecting the preconcentration process were optimized. Under the optimized experimental conditions, the selective preconcentration and determination of as low zinc concentration as 6.5 µg L−1 can be made. The proposed method was successfully applied to the preconcentration and low-level determination of zinc in different water and blood serum samples. [...]
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