Search results

1

Antitumour activity of Salmonella typhimurium VNP20047 in B16(F10) murine melanoma model.

100%

Jazowiecka-Rakus J., Szala S.

Acta Biochimica Polonica

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2004

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vol. 51

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issue 3

851-856

EN

A tumour therapy is proposed based on attenuated Salmonella typhimurium VNP20047 expressing the Escherichia coli cytosine deaminase gene. VNP20047 was administered intravenously to B16(F10) melanoma-bearing C57BL/6 mice. VNP20047 proliferated within tumours and livers regardless of the initial inoculum dose. After 10 days the number of bacteria increased in livers up to 4.2 × 106 cfu/g and decreased in tumours down to 5.9 × 106 cfu/g. VNP20047 at 1 × 105 cfu/mouse, when combined with 5-fluorocytosine, inhibited tumour growth by 85% without prolonging animal survival. Histology studies revealed severe lesions in tumours and livers. These data suggest that S. typhimurium VNP20047 induced inflammatory responses, even though the strain was attenuated.

2

Prevalence and Antibiotic Susceptibility Patterns of Shigella and Salmonella Causing Diarrhoea in Children Below 5 Years at Thika Level Five District Hospital

88%

Kibet S. C., Matiru V. N., Kibaba P. W., Mucheke A., Louis H.

World News of Natural Sciences

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2017

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vol. 11

28-36

EN

The aim of the study was to isolate and identify the extent of Salmonella and Shigella induced diarrhoea in children under five and to determine their antibacterial susceptibility patterns. The method of investigation was a cross-sectional study. Samples were collected from children less than five years old afflicted with diarrhoea coming for treatment at Thika Level Five Hospital, Kiambu County. A total of 80 stool samples were collected. These were specifically examined for Shigella and Salmonella. In doing so, the samples were cultured in MacConkey and Xylose lactose deoxycholate (XLD) agar. The plates were subsequently incubated aerobically at 37 °C overnight. After incubation, suspected Salmonella and Shigella colonies were identified morphologically, marked and the colonies inoculated to biochemical tests for species identification as described in the Medical Laboratory Manual. Following this, colonies derived from purity plating through biochemical testing were sub-cultured onto nutrient agar to obtain pure colonies. The obtained pure colonies were then used to perform susceptibility tests to commonly prescribed antibacterial drugs, including Gentamicin, Ciproflaxicin and Erythromycin. Results showed 10 (12.5%) confirmed positive isolates, where 6 (60%) were Salmonella and 4 (40%) Shigella. With respect to age, both isolates were found to be concentrated more at ages of 1-4 years. Herein, Salmonella – 1-2 years (33.33%), - 2-3years (50%); Shigella – 1-2 years (50%), - 2-3 years (25%). From the study, sex distribution (male or female) of the host isolates was equal (50-50%). Moreover, Ciprofloxacin and Gentamycin were the most potent antibiotics, whereas Ampicillin, Erythromycin, Cotrimoxazole and Sulfamethoxazole were highly resisted. According to study results, Quinolones and Aminogylcosides are the antibiotics of choice for severe diarrhoea illnesses caused by Salmonella and Shigella.

3

Preliminary studies on some avian diseases in Antarctic birds

88%

Leotta G., Cerda R., Coria N. R., Montalti D., Leotta G., Cerda R., Coria N. R., Montalti D.

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2001

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vol. 22

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issue 3-4

4

Prevalence and molecular characterization of Salmonella species associated with piglet diarrhea in North East India

75%

Kylla H., Dutta T. K., Roychoudhury P., Subudhi P. K., Lalsiamthara J.

Polish Journal of Veterinary Sciences

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2019

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issue 4

5

Salmonella and cancer: from pathogens to therapeutics

75%

Chorobik P., Czaplicki D., Ossysek K., Bereta J.

Acta Biochimica Polonica

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2013

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vol. 60

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issue 3

285-297

EN

Bacterial cancer therapy is a concept more than 100 years old - yet, all things considered, it is still in early development. While the use of many passive therapeutics is hindered by the complexity of tumor biology, bacteria offer unique features that can overcome these limitations. Microbial metabolism, motility and sensitivity can lead to site-specific treatment, highly focused on the tumor and safe to other tissues. Activation of tumor-specific immunity is another important mechanism of such therapies. Several bacterial strains have been evaluated as cancer therapeutics so far, Salmonella Typhimurium being one of the most promising. S. Typhimurium and its derivatives have been used both as direct tumoricidal agents and as cancer vaccine vectors. VNP20009, an attenuated mutant of S. Typhimurium, shows significant native toxicity against murine tumors and was studied in a first-in-man phase I clinical trial for toxicity and anticancer activity. While proved to be safe in cancer patients, insufficient tumor colonization of VNP20009 was identified as a major limitation for further clinical development. Antibody-fragment-based targeting of cancer cells is one of the few approaches proposed to overcome this drawback.

6

Personal autonomy in the management of candidal prosthetic joint infection

63%

Villamil-Cajoto I., Eynde-Collado A., Otero L., Villacian Vicedo M.

Open Medicine

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2012

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vol. 7

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issue 4

539-541

EN

The principle of patient autonomy dominates the contemporary debate in medical ethics. Twenty-five years ago, most major medical decisions were left exclusively in the hands of physicians. Such decisions were usually made with beneficent intent but without open discussion, much less the full participation of the patient. Our case involves a patient’s decision to treat an infection with Candida species, the most common fungi affecting humans in a broad spectrum of opportunistic infections, including bones and joints. Only four recent cases of delayed reimplantation arthroplasty for candidal prosthetic joint infection have been reported, and there are no reports, besides ours, of successful non-surgical resolution of candidal prosthetic joint arthritis. We report this case as a patient’s choice with an excellent outcome.

7

Antibiotic Susceptibility Test of Bacteria Isolated From Fruit Juices Sold in Cafes and Restaurants of Debre-Markos Town, North Western Ethiopia

51%

Geta K., Kebede A., Chemedissa M.

World News of Natural Sciences

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2019

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vol. 24

365-371

EN

The prevalence of antimicrobial resistance among food pathogens has increased during recent decades. In this work, pathogenic bacteria such as Staphylococcus aureus, Salmonella spp., Shigella spp., and E. coli were isolated following standard methods. The bacterial isolates were then tested for their sensitivity to common antibiotics using the disc diffusion method on Mueller-Hinton Agar. All of the pathogenic bacteria were found to be resistant to erythromycin and almost all were sensitive to penicillin.

8

Microbiological safety of fruit juices consumed in cafes and restaurants of Debre Markos town, north western Ethiopia

51%

Geta K., Kebede A., Chemedissa M.

World News of Natural Sciences

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2019

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vol. 24

287-298

EN

Improperly prepared fresh fruit and vegetable juices are recognized as an emerging cause of food borne illnesses. Therefore, this study was aimed at evaluating the microbiological safety of fresh fruit juices marketed in Debre Markos town and their hygienic conditions of preparations. Thirty-six fruit juices samples were collected from 6 cafés and restaurants of Debre Markos town and analyzed for total aerobic viable bacterial count (TAVBC), total staphylococcal count (TSC), aerobic spore forming bacterial count (ASFBC), total coliform count (TCC), fecal coliform count (FCC), yeast and mould count (YMC). The spread plate method was used for the isolation of microorganisms on appropriate selective media. All isolates were characterized following standard methods. Bacterial and fungal species were isolated following standard methods, while questionnaires were distributed to 30 juice makers to obtain preliminary information on their hygienic and safety practices. Results show that the mean TAVBC, ASFBC, TSC, yeast and mould, TCC and FCC of mango were 2.2±0.48×106, 0.13±0.04×105, 0.004×105, 1.1±0.2×106, 0.15±0.05×105, 5.7±3.73×104 and 0.06±0.04×104, cfu/ml, respectively. Moreover, the mean of TAVBC, ASFBC, TSC, YMC, TCC, and FCC of avocado juice were 3.6±0.6×106, 0.08±0.02×105, 0.27±0.07×105, 1.2±0.4×106, 0.02±0.01×105, 6.46±3.7×104, and 0.2±0.1×104 cfu/ml, respectively. The bacterial isolates were identified as S. aureus, E. coli, Klebsiella spp. B. cereus, Enterobacter spp., Enterococcous spp., Streptococcus spp., and Serratia spp., while the identities of the fungal isolates were Fusarium spp., Mucor spp. and S. cerevisiae. The results also showed that the microbial loads of most of the fruit juices were higher than the specifications set for fruit juices sold in the Gulf region and other parts of the world. Most venders obtained fruit from the open market and all juice makers lacked special training in food hygiene and safety. Therefore, regular training and health education on food hygiene and safety is recommended for juice handlers to improve the quality of fresh fruit juices in the study area.

Refine search results

Antitumour activity of Salmonella typhimurium VNP20047 in B16(F10) murine melanoma model.

Prevalence and Antibiotic Susceptibility Patterns of Shigella and Salmonella Causing Diarrhoea in Children Below 5 Years at Thika Level Five District Hospital

Preliminary studies on some avian diseases in Antarctic birds

Prevalence and molecular characterization of Salmonella species associated with piglet diarrhea in North East India

Salmonella and cancer: from pathogens to therapeutics

Personal autonomy in the management of candidal prosthetic joint infection

Antibiotic Susceptibility Test of Bacteria Isolated From Fruit Juices Sold in Cafes and Restaurants of Debre-Markos Town, North Western Ethiopia

Microbiological safety of fruit juices consumed in cafes and restaurants of Debre Markos town, north western Ethiopia