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1

Suspension cultures of Pharbitis nil

100%

Szyp I., Tretyn A.

Biotechnologia

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2001

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issue 1

127-130

EN

Suspension cultures are more suitable for physiological, biochemical and molecular investigations than callus cultures grown on solid media, because the former provide more homogeneous system than the latter ones. A large number of plants were found suitable for establishing cell suspension cultures. In this study we describe the obtaining of cell suspension cultures derived from callus induced from cotyledons of Pharbitis nil. Explants isolated from plants grown under inductive and non-inductive conditions were cultured on MS basal medium containing various concentrations and combinations of growth regulators. To initiate the cell suspension culture, small clumps of friable callus obtained from cotyledons were suspended in liquid callusing medium. An initial inoculum density was 2104cells/ml. Every 20 days the cultures were transferred to a fresh medium. The cell number in suspension was determined by direct microscopic counting with haemocytometr. The cell suspension cultures contained both single cells and small cell aggregates.

2

Anthocyanis in suspension cultures orginating from different parts of sweetpotato plant

100%

Konczak-Islam I., Yoshinaga M., Nakatani M., Yamakawa O.

Biotechnologia

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1999

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issue 3

143-153

EN

Anthocyanin accumulating cell lines were established from storage root, leaf and stem explants of sweetpotato (Ipomoea batatas (L.) Lam.), cv. Ayamurasaki. The calli developed on MS basal medium enriched with 2,4-D at 27?C accumulated pigments in the dark. The storage root originated suspension culture generated the highest amount of total anthocyanins expressed as colour value (5.9) followed by the cultures originating from leaf (4.3) and stem (1.7). The cultures displayed similar anthocyanin profile regardless of source of explants. The major pigments derived from suspension cultures appeared with earlier retention time on ODS-column HPLC than the YGM pigments accumulated in vivo, which suggests that they are highly hydrophilic and have simpler chemical structure.

3

Cell suspension obtained from callus or directly from cotyledons of Pharbitis nil Chois

88%

Trejgell A., Wisniewska J., Tretyn A.

Biotechnologia

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2004

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issue 2

219-224

EN

An attempt has been made to obtain cell suspension from callus as well as directly from cotyledons P. nil. Cotyledons of 7-days old sterile seedlings and flower buds excised from 3-week old plants were the material for the induction of callus. The explants were laid out on MS medium supplemented with various combination of plant hormones: BAP (5 mg/l) and NAA (1 mg/l) and supplemented with 3% sucrose or 2% glucose and 1% sucrose, BAP (5 mg/l) and IAA (0,5 mg/l), BAP (0,5 mg/l) and Picloram (1 mg/l), BAP (5 mg/l) and Picloram (0,5 mg/l), 2,4-D (0,125 mg/l).The cultures were grown in continuous white light or in darkness. The callus obtained from cotyledons cultivated in darkness and callus from flower buds cultivated in light on MS medium with BAP (5 mg/l), NAA (1 mg/l), 2% glucose and 1% sucrose were proved useful for obtaining cell suspension. Moreover, an attempt was made to obtain cell suspension directly from cotyledons. Cotyledons were cut into small fragments or were subjected to enzymatic digestion. The cell suspensions were cultivated on MS medium with the addition of BAP (5 mg/l) and IAA (0,5 mg/l) on a shaker at 140 rpm. The increase of cell number was determined by counting the cells every 5 days. In the subsequent subcultures, a decrease of the number of cell divisions was observed.

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3 Biotechnologia

1 2004

1 2001

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2 Tretyn A.

1 Konczak-Islam I.

1 Nakatani M.

1 Szyp I.

1 Trejgell A.

1 Wisniewska J.

1 Yamakawa O.

1 Yoshinaga M.

Suspension cultures of Pharbitis nil

Anthocyanis in suspension cultures orginating from different parts of sweetpotato plant

Cell suspension obtained from callus or directly from cotyledons of Pharbitis nil Chois