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1

Growth kinetics of suspension culture of wheat (Triticum aestivum L.) influenced by sucrose concentration in the medium

100%
Marcinska I., Filek M., Biesaga-Koscielniak J., Pilipowicz M.
Biotechnologia
|
2001
|
issue 2
104-110
EN
The suspension culture was initiated from callus derived from immature inflorescences of winter wheat var Almari. The aim of our investigation was evaluation of the influence of twice increased sucrose concentration in the medium on the physicochemical properties and cell morphology during 15 days of suspension culture. The number of cells, volume, weight, shape and viability were monitored in a few day intervals. Moreover, pH, conductivity and osmolality of medium were measured. The suspensions that were maintained in media containing 30 and 60 g of sucrose per dm3 were characterised by a similar cell viability and growth kinetics tendency. However, from the 4th day of culture, increased concentration of sucrose inhibited the cell proliferation rate. This effect was associated with decreased volume and dry weight of cells, which indicates that sucrose influences the production of cell biomass. The morphological character of cells was independent of the sucrose concentration, the cell area and circularity changed similarly for both media. The area was increased and circularity decreased. Increased concentration of sucrose resulted in lowered pH of the medium during the whole culture period. There was no statistically significant influence of sucrose on the conductivity and osmolality changes in media. The results suggest that higher level of sucrose in the medium can be disadvantageous for growth kinetics of wheat suspension. It was not connected with medium osmotic potential changes or cell-medium ion exchange possibilities.
2

The application of immobilized invertase for continuous production of inverted sucrose

100%
Ginalska G., Belcarz A., Lobarzewski J., Wolski T.
Biotechnologia
|
1999
|
issue 1
226-237
EN
Four inorganic supports (sand, silica gel, crushed pumice and controlled-pore glass) were used for immobilization of invertase. The possibility of application of thus obtained immobilized enzyme in continuous production of inverted sugar was tested. The best support for immobilization of invertase was controlled-pore glass (CPG, average pore size of 31 mm). The conditions of production of inverted sugar by immobilized enzyme were optimised (also in column). Unter the optimised the column (dimensions: 0.7x27 cm) with immobilized invertase was able to degrade sucrose to glucose and fructose in an amount of 60 g/h. Immobilized invertase in the column remained fully active for 120 days.
3

Induction, proliferation and maintenance of embryogenic tissues of Serbian spruce in liquid nitrogen

75%
Hazubska-Przybyl T., Bojarczuk K.
Biotechnologia
|
2010
|
issue 2
96-104
EN
Picea omorika (Pancic.) Purk. is an endemic species, threatened with extinction in the wild. Besides, it is a valuable species for nursery production. Hence it is necessary to protect its genetic resources ex situ. Somatic embryogenesis is a very beneficial micropropagation method because of potentially high regeneration rate and possibilities to maintain embryogenic tissue and somatic embryos in liquid nitrogen (LN). That is why the aim of this study was to induce embryogenic tissue culture of Picea omorika (Pancic.) Purk.
4

Long-term in vitro storage of shoot explants at temperatures above 0C

63%
Lisek A., Orlikowska O. T., Orlikowska T.
Biotechnologia
|
2001
|
issue 3
134-144
EN
Long-term storage of shoots or nodal buds in vitro, in conditions which retard their growth, may be an alternative or supplementary method in the protection of plant resources and in the production of planting material for elite mother stocks. In this paper we describe the most important factors which influence the survival and further behaviour of stored explants.
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