The modern structural methods used in the determination of lipopolysaccharides chemical structures were described. The combination of nuclear magnetic resonance (NMR) spectroscopy and mass spectrometry (MALDI-TOF, FAB and EI) applied to structural analysis of lipopolysaccharides, with a few chosen examples of characteristic original spectra were presented.
The structures of 14 O-specific polysaccharides isolated from Hafnia alvei lipopolysaccharides have been described. The structures of the common core existing in most Hafnia alvei strains examined so far and the core-like trisaccharide found in some Hafnia alvei strains have been also presented.
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