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Effect of 6-benzylaminopurine, cold-hardening and time of transfer of cultures to the storage room on long-term storage of raspberry ?Malling Seedling? shoots in vitro

100%

Lisek A., Orlikowska O. T., Orlikowska T.

Biotechnologia

2001

issue 3

237-242

Shoots of raspberry ?Mallig Seedling? originating from multiplication cultures were stored in vitro at 4C in the dark on a medium with 0,4 mg/l or without 6-benzylaminopurine (BAP), for 18 and 24 months. The cultures were transferred to the storage room immediately after the subculture or after one-week incubation in the growth chamber. A part of the shoots was cold-hardened for one week. Almost all the shoots survived 24 months of storage. The shoots stored on the medium containing BAP formed shoots during storage. Shoot multiplication was poor during the first passage, especially in the result of 24 months storage. Time of transfer and hardening did not influence multiplication capacity instantly.

Long-term in vitro storage of shoot explants at temperatures above 0C

63%

Lisek A., Orlikowska O. T., Orlikowska T.

Biotechnologia

2001

issue 3

134-144

Long-term storage of shoots or nodal buds in vitro, in conditions which retard their growth, may be an alternative or supplementary method in the protection of plant resources and in the production of planting material for elite mother stocks. In this paper we describe the most important factors which influence the survival and further behaviour of stored explants.

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2 Biotechnologia

2 2001

2 Lisek A.

2 Orlikowska O. T.

2 Orlikowska T.

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Effect of 6-benzylaminopurine, cold-hardening and time of transfer of cultures to the storage room on long-term storage of raspberry ?Malling Seedling? shoots in vitro

Long-term in vitro storage of shoot explants at temperatures above 0C