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Field performance of transgenic clones obtained from potato cv. Irga

100%

Flis B., Zimnoch-Guzowska E.

Journal of Applied Genetics

2000

vol. 41

issue 2

81-90

The aim of the study reported here was to evaluate the performance and variability of transgenic potato clones. For the genetic transformation the Polish potato cultivar Irga was chosen in order to improve resistance to a necrotic strain of potato virus Y (PVYN ) by introducing to its genome a truncated gene coding PVYN replicase (in sense and antisense orientations). Transgenic plants and clones derived from them were propagated and the obtained tubers were planted in a replicated field trial. The several agronomic and morphological traits were evaluated and compared with measurements for non-transgenic control plants. The traits of transgenic clones showed a much greater variability than non-transgenic plants. The variability depended on the type of the introduced construct (in this case it was the orientation of the construct). None of the transgenic clones turned out to be completely true to type and resistant to PVYN, but some resistant clones expressed deviations in a small proportion of the traits. Usually, deviations were observed for those traits whose inheritance is characterised by a large environmental component. Genetic transformation is an effective method for introducing resistance. However, the method causes a great variability, which makes selection among transgenic clones a necessary step in breeding of an improved transgenic cultivar. Such selection has many similarities with selection done among traditionally obtained clones.

Marker-assisted selection of diploid and tetraploid potatoes carrying Rpi-phu1, a major gene for resistance to Phytophthora infestans

75%

Sliwka J., Jakuczun H., Kaminski P., Zimnoch-Guzowska E.

Journal of Applied Genetics

2010

vol. 51

issue 2

133-140

The Rpi-phu1 gene originates from an interspecific hybrid between Solanum stenotomum and S. phureja, and confers a high level of resistance to Phytophthora infestans (late blight) in potato. The Rpi-phu1 was introduced by crossing at the diploid level into the S. tuberosum gene pool and then transferred to the tetraploid level by means of 2n gametes. Tetraploid lines carrying the Rpi-phu1 were selected for further crosses. A molecular marker GP94, linked in mapping population 97?30 with the Rpi-phu1 (6.4 cM), was applied to other unselected populations (2 diploid and 1 tetraploid), and was shown to be useful in marker-assisted selection (MAS) of the resistant individuals. GP94 was applied also in commercial breeding in 2 tetraploid populations. Although the marker allele of GP94, characteristic for the Rpi-phu1 presence, was rare in the S. tuberosum gene pool, it seemed to be common in 2 other sources of resistance to late blight: S. ruiz-ceballosii and S. kurtzianum.

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2 Journal of Applied Genetics

1 2010

1 2000

2 Zimnoch-Guzowska E.

1 Flis B.

1 Jakuczun H.

1 Kaminski P.

1 Sliwka J.

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Field performance of transgenic clones obtained from potato cv. Irga

Marker-assisted selection of diploid and tetraploid potatoes carrying Rpi-phu1, a major gene for resistance to Phytophthora infestans